Deglycosylation by gaseous hydrogen fluoride of mucus glycoproteins immobilized on nylon membranes and in microtiter wells.

• Strongly reacting antibodies specific for defined mucin gene products are often directed against the mucin protein backbone of the heavily glycosylated serine/threonine rich regions. A prerequisite for the use of such antibodies is often the complete removal of the oligosaccharides from the protein. This paper describes an efficient one-step deglycosylation method using gaseous hydrogen fluoride on nylon blotting membranes and microtiter wells.

Ämnesord

MEDICIN OCH HÄLSOVETENSKAP  -- Medicinsk bioteknologi -- Medicinsk bioteknologi (hsv//swe)

MEDICAL AND HEALTH SCIENCES  -- Medical Biotechnology -- Medical Biotechnology (hsv//eng)

Nyckelord

Antibodies

Monoclonal

Blotting

Western

Gases

Hydrofluoric Acid

Membranes

Artificial

Mucin-1

chemistry

immunology

Mucin-2

Mucin-3

Mucins

chemistry

immunology

Nylons

Oligosaccharides

Publikations- och innehållstyp

ref (ämneskategori)

art (ämneskategori)