| 1. |
- Uhlén, Mathias, et al.
(author)
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A human protein atlas for normal and cancer tissues based on antibody proteomics
- 2005
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In: Molecular & Cellular Proteomics. - 1535-9476 .- 1535-9484. ; 4:12, s. 1920-1932
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Journal article (peer-reviewed)abstract
- Antibody-based proteomics provides a powerful approach for the functional study of the human proteome involving the systematic generation of protein-specific affinity reagents. We used this strategy to construct a comprehensive, antibody-based protein atlas for expression and localization profiles in 48 normal human tissues and 20 different cancers. Here we report a new publicly available database containing, in the first version, similar to 400,000 high resolution images corresponding to more than 700 antibodies toward human proteins. Each image has been annotated by a certified pathologist to provide a knowledge base for functional studies and to allow queries about protein profiles in normal and disease tissues. Our results suggest it should be possible to extend this analysis to the majority of all human proteins thus providing a valuable tool for medical and biological research.
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| 2. |
- Van Odijk, Jenny, 1969, et al.
(author)
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Measurements of eosinophil activation before and after food challenges in adults with food hypersensitivity
- 2006
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In: Int Arch Allergy Immunol. - : S. Karger AG. - 1018-2438 .- 1423-0097. ; 140:4, s. 334-41
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Journal article (peer-reviewed)abstract
- BACKGROUND: Objective assessment of inflammatory reactions in the gastrointestinal tract could be useful in the diagnosis of food hypersensitivity. The aim of the present study was to investigate the involvement of eosinophils and mast cells in the inflammatory response of patients with food hypersensitivity before and after food challenges. METHODS: Eleven patients (4 with IgE-mediated allergy and 7 without) with food hypersensitivity and positive double-blind, placebo-controlled food challenge were subjected to food challenge in a single-blinded fashion. Four subjects with no known food hypersensitivity were recruited as controls. Placebo was given after a 1-week washout period followed by an active dose. Stool, urinary and serum samples were collected and symptoms were recorded in a diary. Fecal samples were analyzed for eosinophil protein X (F-EPX) and tryptase; urinary samples for EPX (U-EPX) and leukotriene E4 (U-LTE4) and serum samples were analyzed for eotaxin and food-specific IgE antibodies. RESULTS: Patients with IgE-mediated food allergy had increased levels of F-EPX compared to controls and tended to have lower serum levels of eotaxin compared to non-allergic patients and controls. U-LTE4 was significantly higher in allergic patients compared to non-allergic patients after challenge. Moreover, F-EPX correlated to U-LTE4 (p = 0.011). Reported symptoms, abdominal pain, distension, flatulence and nausea were similar in the allergic and non-allergic patients. CONCLUSION: The results strongly indicate that eosinophils are activated in the gastrointestinal tract of food-allergic patients but not in patients with non-allergic food hypersensitivity. Due to the inconsistent pattern of symptoms after placebo and active food challenge, it was not possible to relate the levels of inflammation markers to the recorded symptoms.
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| 3. |
- Hansson, Anders, et al.
(author)
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The Lim-only protein LMO2 acts as a positive regulator of erythroid differentiation
- 2007
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In: Biochemical and Biophysical Research Communications - BBRC. - : Elsevier BV. - 0006-291X .- 1090-2104. ; 364:3, s. 675-681
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Journal article (peer-reviewed)abstract
- LMO2, a member of the LIM-only protein family, is essential for the regulation of hematopoietic stem cells and formation of erythroid cells. It is found in a transcriptional complex comprising LMO2, TAL1, E47, GATA-1, and LDB1 which regulates erythroid genes. While TAL1 has been shown to induce erythroid differentiation, LMO2 appears to suppress fetal erythropoiesis. In addition to LMO2, the closely related LMO4 gene is expressed in hematopoietic cells, but has unknown functions. Here we demonstrate that LMO2 and LMO4 are expressed at the same level in erythroid colonies from mouse bone marrow, implying a function in erythroid differentiation. However, while LMO2 induced erythroid differentiation, LMO4 had no such effect. Interestingly, both LMO2 and TAL1 were able to partially suppress myeloid differentiation, implying that they activate erythroid differentiation in uncommitted bone marrow progenitors. Both LMO2 and LMO4 interacted strongly to LDB1, which was required for their localization to the nucleus. © 2007 Elsevier Inc. All rights reserved.
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| 4. |
- Hansson, Helena, et al.
(author)
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An intervention program for university students who have parents with alcohol problems: A randomized controlled trial
- 2006
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In: Alcohol and Alcoholism. - : Oxford University Press (OUP). - 1464-3502 .- 0735-0414. ; 41:6, s. 655-663
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Journal article (peer-reviewed)abstract
- Aim: To study the effects of alcohol and coping intervention among University students who have parents with alcohol problems. Methods: A total of 82 university students (56 women and 22 men, average age 25) with at least one parent with alcohol problems were included. The students were randomly assigned to one of three programs: (i) alcohol intervention program, (ii) coping intervention program, and (iii) combination program. All programs were manual based and individually implemented during two 2-h sessions, 4 weeks apart. This assessment contained both a face-to-face interview and six self-completion questionnaires; AUDIT, SIP, EBAC, coping with parents' abuse questionnaire, SCL-90 and ISSI. Follow-up interviews were conducted after 1 year. Results: All participants finished the baseline assessment, accepted and completed the intervention, while 95% of the students completed the 12-month follow-up assessment. The two groups that received alcohol intervention improved their drinking pattern significantly more than the group that did not receive alcohol intervention [change of standardized scores -0.27 (CI -0.53 to -0.03)]. The groups receiving coping intervention did not differ from the group not receiving coping intervention concerning their ability to cope with their parents' alcohol problems. Nor did they differ regarding changes in their own mental health or in their social interaction capacity. Conclusion: The intervention improved drinking patterns in adult children of alcoholics.
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| 5. |
- Hansson, Helena, et al.
(author)
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Two-year outcome of an intervention program for university students who have parents with alcohol problems: A randomized controlled trial
- 2007
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In: Alcoholism: Clinical and Experimental Research. - : Wiley. - 0145-6008 .- 1530-0277. ; 31:11, s. 1927-1933
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Journal article (peer-reviewed)abstract
- Background: Only a few intervention studies aiming to change high-risk drinking behavior have involved university students with heredity for alcohol problems. This study evaluated the effects after 2 years on drinking patterns and coping behavior of intervention programs for students with parents with alcohol problems. Method: In total, 82 university students (57 women and 25 men, average age 25 years) with at least 1 parent with alcohol problems were included in the study. The students were randomly assigned to 1 of the 3 programs: (i) alcohol intervention program, (ii) coping intervention program, or (iii) combination program. All the 3 intervention programs were manual based and individually implemented during 2 2-hour sessions, 4 weeks apart. Before the participants were randomly assigned, all were subjected to an individual baseline assessment. This assessment contained both a face-to-face interview and 6 self-completion questionnaires: the Alcohol Use Disorders Identification Test, estimated Blood Alcohol Concentration, Short Index of Problems, the Symptom Checklist-90, Coping with Parents' Abuse Questionnaire, and The Interview Schedule for Social Interaction (ISSI). Follow-up interviews were conducted after 1 and 2 years, respectively. The results after 1 year have previously been reported. Results: All participants finished the baseline assessment, accepted and completed the intervention. Ninety-five percent of the students completed the 24-month follow-up assessment. Only the group receiving the combination program continued to improve their drinking pattern significantly (p < 0.05) from the 12-month follow-up to the 24-month follow-up. The improvements in this group were significantly better than in the other 2 groups. The group receiving only alcohol intervention remained at the level of improvement achieved at the 12-month follow-up. The improvements in coping behavior achieved at the 12-month follow-up remained at the 24-month follow-up for all the 3 groups, i.e., regardless of intervention program. Conclusion: Positive effects of alcohol intervention between 1 and 2 years were found only in the combined intervention group, contrary to the 1-year results with effects of alcohol intervention with or without a combination with coping intervention.
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| 6. |
- Hansson, Katarina, et al.
(author)
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Belastning av miljögifter på vatten : Kartläggning av källor till miljögifter
- 2009
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Reports (other academic/artistic)abstract
- Denna rapport sammanställer information om belastningen av organiska miljögifter och metaller på ytvatten. I projektet har 16 olika ämnen och ämnesgrupper valts ut som prioriterade. Dessa ämnen är upptagna i ramdirektivet för vatten och/eller i HELCOMs Baltic Sea Action Plan (BSAP) och är intressanta ur både ett nationellt och ett regionalt perspektiv.I syfte att ge förslag till en beräkningsmetodik har en inventering av datatillgången genomförts. I den sammanställningen presenteras vilka miljöövervakningsdata och övriga datakällor som kan ligga till grund för emissionsberäkningar. I samband med det identifierades dataluckor.Uppgifter om punktutsläpp av organiska miljögifter till vatten är knapphändiga i EMIR-databasen. Med undantag för vissa enstaka ämnen och anläggningar finns det i EMIR-databasen endast data gällande utsläppen av metaller. Punktkällor för utsläpp till vatten är industrier, gruvor, avloppsreningsverk, värmevärk samt icke rapporterande punktkällor, som t.ex. enskilda avlopp. De diffusa källorna av organiska ämnen och metaller är atmosfärisk deposition, läckage från jordbruks- och skogsmark, dagvatten, lakvatten från deponier samt re-emission från sediment.I rapporten föreslås en stegvis metodik för kvantifiering av utsläppen av organiska ämnen och metaller till vatten. Beräkning bör i första hand baseras på rena emissionsdata. Om dessa inte finns tillgängliga bör omräkning från miljöövervakningsdata tillämpas. Screeningdata och övriga regionala data och resultat från enskilda forskningsrapporter kan utnyttjas i brist på annan information, men innebär att trendanalyser av data inte kan genomföras. I de fall där rena emissionsdata och miljöövervakningsdata saknas, kan statistik om användning av varor och produkter innehållande de aktuella ämnena utnyttjas som ett mått på trender eller på den diffusa belastningen.I rapporten har vi kvantifierat belastningen av metaller och vissa organiska ämnen och ämnesgrupper från atmosfäriska depositionen och rapporterande punktkällor. Dessa resultat pekar på att för metallerna är bidraget från atmosfären större än utsläppen från kända punktkällor, men det är också viktigt att poängtera att de framtagna resultaten är delvis beräknade från mycket osäkert underlag. De diffusa utsläppen utgör även en viktig spridningskälla till förekomst av organiska ämnen i ytvattnet.För att i framtiden kunna genomföra bra emissionsberäkningar av miljögifter till vatten bör metodiken för de enskilda källorna utvecklas. Dessutom krävs det vidare arbete med metodikutvecklingen och bättre datatillgång.
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| 7. |
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| 8. |
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| 9. |
- Kawabe, Akira, et al.
(author)
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Centromere Locations and Associated Chromosome Rearrangements in Arabidopsis lyrata and A. thaliana
- 2006
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In: Genetics. - Baltimore, MD, USA : The Genetics Society. - 0016-6731 .- 1943-2631. ; 173:3, s. 1613-1619
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Journal article (peer-reviewed)abstract
- We analyzed linkage and chromosomal positions of genes in A. lyrata ssp. petraea that are located near the centromere (CEN) regions of A. thaliana, using at least two genes from the short and long arms of each chromosome. In our map, genes from all 10 A. thaliana chromosome arms are also tightly linked in A. lyrata. Genes from the regions on the two sides of CEN5 have distant map localizations in A. lyrata (genes on the A. thaliana short-arm genes are on linkage group AL6, and long-arm genes are on AL7), but genes from the other four A. thaliana centromere regions remain closely linked in A. lyrata. The observation of complete linkage between short- and long-arm centromere genes, but not between genes in other genome regions that are separated by similar physical distances, suggests that crossing-over frequencies near the A. lyrata ssp. petraea centromere regions are low, as in A. thaliana. Thus, the centromere positions appear to be conserved between A. thaliana and A. lyrata, even though three centromeres have been lost in A. thaliana, and the core satellite sequences in the two species are very different. We can now definitively identify the three centromeres that were eliminated in the fusions that formed the A. thaliana chromosomes. However, we cannot tell whether genes were lost along with these centromeres, because such genes are absent from the A. thaliana genome, which is the sole source of markers for our mapping.
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| 10. |
- Kawabe, Akira, et al.
(author)
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Comparative gene mapping in Arabidopsis lyrata chromosomes 6 and 7 and A. thaliana chromosome IV : Evolutionary history, rearrangements and local recombination rates
- 2006
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In: Genetical Research. - 0016-6723 .- 1469-5073. ; 88:1, s. 45-56
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Journal article (peer-reviewed)abstract
- We have increased the density of genetic markers on the Arabidopsis lyrata chromosomes AL6 and AL7 corresponding to the A. thaliana chromosome IV, in order to determine chromosome rearrangements between these two species, and to compare recombination fractions across the same intervals. We confirm the two rearrangements previously inferred (a reciprocal translocation and a large inversion, which we infer to be pericentric). By including markers around the centromere regions of A. thaliana chromosomes IV and V, we localize the AL6 centromere, and can localize the breakpoints of these chromosome rearrangements more precisely than previously. One translocation breakpoint was close to the centromere, and the other coincided with one end of the inversion, suggesting that a single event caused both rearrangements. At the resolution of our mapping, apart from these rearrangements, all other markers are in the same order in A. lyrata and A. thaliana. We could thus compare recombination rates in the two species. We found slightly higher values in A. thaliana, and a minimum estimate for regions not close to a centromere in A. lyrata is 4-5 centimorgans per megabase. The mapped region of AL7 includes the self-incompatibility loci (S-loci), and this region has been predicted to have lower recombination than elsewhere in the genome. We mapped 17 markers in a region of 1·23 Mb surrounding these loci, and compared the approximately 600 kb closest to the S-loci with the surrounding region of approximately the same size. There were significantly fewer recombination events in the closer than the more distant region, supporting the above prediction, but showing that the low recombination region is very limited in size. © 2006 Cambridge University Press.
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