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- Agrell, Beata, 1944, et al.
(författare)
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Kärleken i vredens mask, vreden i kärlekens : samtalet fortsätter om Lars Ahlin, om Hedvig och hennes son
- 1999
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Ingår i: Vår lösen. - 0346-4679. ; 90 (1999):1, s. 131-146
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Tidskriftsartikel (övrigt vetenskapligt/konstnärligt)abstract
- Om religiösa motiv i Lars Ahlins böcker med utgångspunkt i "Natt i marknadstältet". I samtalet deltog Gunnar D. Hansson, Carl Axel Aurelius, Beata Agrell, Gustaf Wingren, Eva Haettner Aurelius, Anders Tyrberg, Peter Myrman, Lasse Berndes, Edgar Almén och Kerstin Bergman. Den ursprungliga rubriken var Livets långa brudslöja och samtalet ägde rum på ett Gustaf Wingren-symposium i Linköping 1997. I samtalet ingick även två inledningar av Carl Axel Aurelius och Gunnar D. Hansson, bägge publicerade i Vår lösen 1999:1 under rubriken Livets långa brudslöja.
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| 4. |
- Hansson, Ulla-Britt, et al.
(författare)
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Antigen-binding sites dominate the surface properties of antibodies
- 1996
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Ingår i: Immunotechnology. - 1380-2933. ; 2:4, s. 276-276
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Tidskriftsartikel (refereegranskat)abstract
- We have found a remarkable relationship between the specificity of antibodies and their chromatographic behavious upon liquid-liquid partition chromatography (LLPC). Well characterized human and murine monoclonal antibodies and Fab/Fv fragments thereof as well as mouse/human chimeric antibodies were employed. While, lgG 1, 2 and 4 antibodies with identical specificities (affinity constants) have identical partition properties, lgG antibodies with different partition properties reacted with different partition epitopes or had different affinities against the same epitope. Hence, the surface properties of the antigen binding sites dominate over all other surfaces of the free antibody molecule. LLPC may also be used to detect conformational changes occuring upon binding of antigen by antibody. Antigen-antibody complexes formed by different lgG antibodies against a large antigen like HSA all had similar surface properties. different from those of both antigen and antibody. In contrast, the surface properties of complexes formed by small antigens haptens are related to those of the lgG antibody. In addition, antigen-antibody complexes were found to have similar surface properties irrespective of the molar ratio of antigen to antibody at which the complexes had been formed.
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- Rånby, Mats, et al.
(författare)
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Isocitrate as Calcium Ion Activity Buffer in Coagulation Assays
- 1999
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Ingår i: Clinical Chemistry. - 0009-9147 .- 1530-8561. ; 45:8, s. 1176-1180
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Tidskriftsartikel (refereegranskat)abstract
- Background: Ca2+ activity close to the physiological concentration of 1.3 mmol/L is essential in blood coagulation. Is this also true for the performance of global diagnostic coagulation assays? We searched for compounds that would buffer Ca2+ activity at ∼1.3 mmol/L without disturbing coagulation reactions and investigated whether such Ca2+ buffering improves diagnostic efficacy in global diagnostic coagulation tests.Methods: Buffering was investigated by mixing CaCl2 and 11 candidate compounds and determining Ca2+ activity. The best candidates were added to mixtures of plasma and thromboplastin to detect interference with coagulation reactions. The best of these candidates, isocitrate, was used to modify an activated partial thromboplastin time (APTT), buffering final Ca2+ activity to ∼1.3 mmol/L. Plasma samples from 22 healthy individuals and 120 patients were analyzed with original and modified APTT to determine whether diagnostic efficacy was improved.Results: Two suitable Ca2+ buffers, citrate and isocitrate, were found. Isocitrate was preferred as being less coagulation inhibitory, a better Ca2+ buffer, and possibly a better anticoagulant. The isocitrate-modified APTT showed a final Ca2+ activity of 1.60 ± 0.07 mmol/L, compared with 2.73 ± 0.20 mmol/L for the original APTT. The means and SDs for the healthy individuals were determined for both procedures, and the values were used to express patient deviation from normality (difference from mean divided by SD). The deviation was greater for the modified APTT; 4.3 ± 5.7, compared with 3.6 ± 5.0 (P <0.005) for the original APTT.Conclusions: Isocitrate can be used to buffer Ca2+ activity at physiological concentrations and can serve as an anticoagulant. APTT with isocitrate-buffered Ca2+ activity shows signs of improved diagnostic efficacy.
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