| 1. |
- Belyaev, I Y, et al.
(author)
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Effects of ethidium bromide on DNA loop organisation in human lymphocytes measured by anomalous viscosity time dependence and single cell gel electrophoresis
- 1999
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In: Biochimica et Biophysica Acta - General Subjects. - 0304-4165 .- 1872-8006. ; 1428:2-3, s. 348-356
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Journal article (peer-reviewed)abstract
- The effects of ethidium bromide (EtBr) on human lymphocytes were studied by the method of anomalous viscosity time dependence (AVTD) and by the comet assay. EtBr at low concentrations increased the maximum viscosity and time of radial migration as measured with AVTD at neutral conditions of lysis. A pronounced relaxation of DNA loops was observed with the neutral comet assay. The maximal comet length corresponded to 2 Mb DNA loops. At high concentrations of EtBr, 2. mg/ml, significant reduction in AVTD below control level was seen that suggested hypercondensation of chromatin. The hypercondensation was directly observed with the neutral comet assay. EtBr did not induce DNA strand breaks as measured by the alkaline comet assay. The hypercondensed nuclei could be decondensed by irradiation with gamma-rays or exposure to light. The data provide evidence that EtBr at high concentrations resulted in hypercondensation of chromatin below control level. The comet assay confirmed that the increase in AVTD peaks deals with relaxation of loops and AVTD decrease is caused by chromatin condensation. The prediction of the AVTD theory for a correlation between time of radial migration and condensation of chromatin was verified. Further, the data show that the comet assay at neutral conditions of lysis is rather sensitive to DNA loop relaxation in the absence of DNA damage. Finally, donor specificity was found for the hypercondensation.
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| 2. |
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| 3. |
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| 5. |
- Nilsson-Wikmar, L, et al.
(author)
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Back pain in women post-partum is not a unitary concept
- 1999
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In: Physiotherapy research international : the journal for researchers and clinicians in physical therapy. - : Wiley. - 1358-2267. ; 4:3, s. 201-13
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Journal article (peer-reviewed)
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| 6. |
- Pettersson, Håkan, 1956-, et al.
(author)
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Summary of IAEA-MEL's investigation of Kara Sea radioactivity and radiological assessment
- 1997
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In: Marine Pollution Bulletin. - 0025-326X .- 1879-3363. ; 35:7-12, s. 235-241
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Journal article (peer-reviewed)abstract
- IAEA-MEL participated in five expeditions to the Kara Sea with the aim of assessing the radiological consequences of dumped radioactive wastes in the Novaya Zemlya Bays and Trough. The programme included sampling, in-situ underwater investigations, laboratory analyses of water, sediment and biota samples, the development of a marine radioactivity database, modelling and radiological assessment, the organization of intercomparison exercises and the evaluation of distribution coefficients. Radiometric investigations have shown that no radiologically significant environmental contamination has occurred. Leakages which have led to locally increased levels of radionuclides in sediment have only been observed in Stepovoy and Abrosimov Bays. Computer modelling results suggest that only radiological effects on local and regional scales may be of importance. The global radiological impact of the disposals in the Arctic Seas will be negligible.
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