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- Ekeberg, Tomas, 1983-, et al.
(författare)
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Observation of a single protein by ultrafast X-ray diffraction
- 2024
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Ingår i: Light. - : Springer Nature. - 2095-5545 .- 2047-7538. ; 13:1
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Tidskriftsartikel (refereegranskat)abstract
- The idea of using ultrashort X-ray pulses to obtain images of single proteins frozen in time has fascinated and inspired many. It was one of the arguments for building X-ray free-electron lasers. According to theory, the extremely intense pulses provide sufficient signal to dispense with using crystals as an amplifier, and the ultrashort pulse duration permits capturing the diffraction data before the sample inevitably explodes. This was first demonstrated on biological samples a decade ago on the giant mimivirus. Since then, a large collaboration has been pushing the limit of the smallest sample that can be imaged. The ability to capture snapshots on the timescale of atomic vibrations, while keeping the sample at room temperature, may allow probing the entire conformational phase space of macromolecules. Here we show the first observation of an X-ray diffraction pattern from a single protein, that of Escherichia coli GroEL which at 14 nm in diameter is the smallest biological sample ever imaged by X-rays, and demonstrate that the concept of diffraction before destruction extends to single proteins. From the pattern, it is possible to determine the approximate orientation of the protein. Our experiment demonstrates the feasibility of ultrafast imaging of single proteins, opening the way to single-molecule time-resolved studies on the femtosecond timescale.
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| 2. |
- Menkveld, Albert J., et al.
(författare)
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Nonstandard Errors
- 2024
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Ingår i: JOURNAL OF FINANCE. - : Wiley-Blackwell. - 0022-1082 .- 1540-6261. ; 79:3, s. 2339-2390
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Tidskriftsartikel (refereegranskat)abstract
- In statistics, samples are drawn from a population in a data-generating process (DGP). Standard errors measure the uncertainty in estimates of population parameters. In science, evidence is generated to test hypotheses in an evidence-generating process (EGP). We claim that EGP variation across researchers adds uncertainty-nonstandard errors (NSEs). We study NSEs by letting 164 teams test the same hypotheses on the same data. NSEs turn out to be sizable, but smaller for more reproducible or higher rated research. Adding peer-review stages reduces NSEs. We further find that this type of uncertainty is underestimated by participants.
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| 3. |
- Santos, Luis, et al.
(författare)
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Marine Fuel Regulations and Engine Emissions: Impacts on Physicochemical Properties, Cloud Activity and Emission Factors
- 2024
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Ingår i: JOURNAL OF GEOPHYSICAL RESEARCH-ATMOSPHERES. - 2169-897X .- 2169-8996. ; 129:5
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Tidskriftsartikel (refereegranskat)abstract
- Marine regulations aim to reduce sulfur and nitrogen exhaust emissions from maritime shipping. Here, two compliance pathways for reducing sulfur dioxide emissions, fuel sulfur content reduction and exhaust wet scrubbing, are studied for their effects on physicochemical properties and cloud forming abilities of engine exhaust particles. A test-bed diesel engine was utilized to study fresh exhaust emissions from combustion of non-compliant, high sulfur content fuel with (WS) and without (HiS) the usage of a wet scrubber as well as a regulatory compliant, low sulfur content fuel (LoS). Particle number emissions are decreased by approximate to 99% when switching to LoS due to absence of 20-30 nm sulfate rich particles. While number emissions for WS are also decreased, a shift in the sulfate mode toward larger sizes was found to increase particle mass emission factors by at least 31%. Changes in the mixing state induced by the compliance measures are reflected in the hygroscopicity of the exhaust particles. Fuel sulfur reduction decreased cloud condensation nuclei emissions by at least 97% due to emissions of primarily hydrophobic soot particles. Wet scrubbing increased those emissions, mainly driven by changes in particle size distributions. Our results indicate that both compliance alternatives have no obvious impact on the ice forming abilities of 200 nm exhaust particles. These detailed results are relevant for atmospheric processes and might be useful input parameters for cloud-resolving models to investigate ship aerosol-cloud interactions and to quantify the impact of shipping on radiative budgets from local to global scales. We investigate how two pathways to comply with international regulations, aiming to reduce emissions of atmospheric pollutants from ships, alter properties of exhaust particles. Both investigated compliance measures (i.e., combustion of cleaner, low sulfur content fuels and aftertreatment of exhaust from a high sulfur content fuel via wet scrubbing) have substantial impacts on the chemical and physical properties of these particles. We find that, while both alternatives reduce the total number of emitted particles substantially, the effect on emissions of cloud forming particles is path dependent. While fuel sulfur content reduction decreased the number of cloud forming particles by about 97%, wet scrubbing led to a strong increase in emissions, suggesting that the measures can have substantial and opposing impacts on local cloud formation and evolution. Low sulfur content fuels can reduce emissions of ultrafine particulate matter significantly Exhaust wet scrubbing can lead to shifts in particle size distributions and reduce soot emissions Usage of low sulfur content fuels or exhaust wet scrubbing have opposing effects on CCN activity of ship exhaust particles
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