| 1. |
- Imanishi, T., et al.
(författare)
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Integrative annotation of 21,037 human genes validated by full-length cDNA clones
- 2004
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Ingår i: PLoS biology. - : Public Library of Science (PLoS). - 1544-9173 .- 1545-7885. ; 2:6, s. 856-875
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Tidskriftsartikel (refereegranskat)abstract
- The human genome sequence defines our inherent biological potential; the realization of the biology encoded therein requires knowledge of the function of each gene. Currently, our knowledge in this area is still limited. Several lines of investigation have been used to elucidate the structure and function of the genes in the human genome. Even so, gene prediction remains a difficult task, as the varieties of transcripts of a gene may vary to a great extent. We thus performed an exhaustive integrative characterization of 41,118 full-length cDNAs that capture the gene transcripts as complete functional cassettes, providing an unequivocal report of structural and functional diversity at the gene level. Our international collaboration has validated 21,037 human gene candidates by analysis of high-quality full-length cDNA clones through curation using unified criteria. This led to the identification of 5,155 new gene candidates. It also manifested the most reliable way to control the quality of the cDNA clones. We have developed a human gene database, called the H-Invitational Database (H-InvDB; http://www.h-invitational.jp/). It provides the following: integrative annotation of human genes, description of gene structures, details of novel alternative splicing isoforms, non-protein-coding RNAs, functional domains, subcellular localizations, metabolic pathways, predictions of protein three-dimensional structure, mapping of known single nucleotide polymorphisms (SNPs), identification of polymorphic microsatellite repeats within human genes, and comparative results with mouse full-length cDNAs. The H-InvDB analysis has shown that up to 4% of the human genome sequence (National Center for Biotechnology Information build 34 assembly) may contain misassembled or missing regions. We found that 6.5% of the human gene candidates (1,377 loci) did not have a good protein-coding open reading frame, of which 296 loci are strong candidates for non-protein-coding RNA genes. In addition, among 72,027 uniquely mapped SNPs and insertions/deletions localized within human genes, 13,215 nonsynonymous SNPs, 315 nonsense SNPs, and 452 indels occurred in coding regions. Together with 25 polymorphic microsatellite repeats present in coding regions, they may alter protein structure, causing phenotypic effects or resulting in disease. The H-InvDB platform represents a substantial contribution to resources needed for the exploration of human biology and pathology.
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| 2. |
- Fahlander, Claes, et al.
(författare)
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Excited States in 103Sn: Neutron Single-particle Energies with Respect to 100Sn
- 2001
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Ingår i: Physical Review C (Nuclear Physics). - 0556-2813 .- 1089-490X. ; 63:2
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Tidskriftsartikel (refereegranskat)abstract
- Gamma-ray lines from Sn-103 have been identified for the first time using EUROBALL and ancillary detectors. The level scheme of Sn-103 has been established by means of particle-gated gamma gamma coincidences. The energy spacing between the g(7/2) and d(5/2) neutron single-particle orbitals is determined from the excited states in Sn-103.
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| 3. |
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| 4. |
- Palacz, M., et al.
(författare)
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Investigations of Neutron Deficient Nuclei Close to 100Sn with EUROBALL
- 2001
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Ingår i: Acta Physica Polonica. Series B: Elementary Particle Physics, Nuclear Physics, Statistical Physics, Theory of Relativity, Field Theory. - 0587-4254 .- 1509-5770. ; 32, s. 999-1003
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Tidskriftsartikel (refereegranskat)abstract
- Excited states of nuclei in the vicinity of Sn-100 have been studied using: the EUROBALL detector array. Gamma-ray lines from Sn-103 have been identified for the first time, and a level scheme of low-lying excited states of Sn-103 has been established. New constraints on energies of single particle orbitals with respect to the Sn-100 core are obtained.
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| 5. |
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| 6. |
- Arndt, A, et al.
(författare)
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Ankle and subtalar kinematics measured with intracortical pins during the stance phase of walking
- 2004
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Ingår i: Foot & ankle international. - : SAGE Publications. - 1071-1007 .- 1944-7876. ; 25:5, s. 357-364
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Tidskriftsartikel (refereegranskat)abstract
- The absence of external landmarks on the talus has rendered the description of ankle and subtalar joint kinematics difficult. Abnormal motion at these joints has, however, been implied in the etiology of an array of lower extremity overuse injuries. Methods: Intracortical pins were inserted under local anesthesia in the tibia, talus, and calcaneus with external marker clusters traced by a video motion analysis system. Kinematic data were collected during walking trials on a flat surface for three subjects. Gait pattern was monitored by comparison of ground reaction force curves during stance phase with and without the pins inserted. Results: Results were presented in terms of helical axis orientation for both joints and the component rotations about these axes. Large intersubject differences were seen in both ankle and subtalar joint helical axis orientation. Maximum rotations over the complete stance phase for the ankle and subtalar joints respectively were: eversion/inversion, 6.3° and 8.3°; dorsiflexion/plantarflexion, 18.7° and 3.7°; and abduction/adduction, 5.0° and 6.1°. Conclusions: The majority of ankle eversion/inversion occurred at the subtalar joint; however, the ankle component cannot be ignored. Abduction/adduction range of motion at the subtalar joint was surprisingly high, indicating that this component motion during walking is not purely attributable to the ankle joint. Future research should include greater subject numbers in order to present more universally applicable results. Clinical Relevance: The in vivo kinematics of the talus during weightbearing activity are poorly understood. The description of this motion may assist in the structuring of clinical rehabilitation and in the design and insertion of ankle joint prostheses.
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| 7. |
- Hashimoto, A, et al.
(författare)
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Simulation of Convective Drying of Wet Porous Materials
- 2003
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Ingår i: Drying Technology. - 1532-2300. ; 21:8, s. 1411-1431
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Tidskriftsartikel (refereegranskat)abstract
- A simulation model for convective drying of wet porous materials was developed. For the simulation, we measured the moisture diffusivities within them and applied a modified Dubinin-Astakhov equation to the moisture sorption data for a membrane filter. The simulation results not accounting for internal mass transfer resistance were quite different from the experimental ones. The drying characteristics calculated by a shrinking core model with effective moisture diffusivity represented a much lower drying rate and much higher temperatures, respectively, than the experimental ones. This meant that we must consider the plural moisture transport mechanisms within the samples. Therefore, we calculated the drying rate and temperatures with an apparent overall mass transfer coefficient damping with a decrease in the moisture content. The results accounting for the hygroscopic effects broadly agreed with the experimental ones by the evaluation.
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| 8. |
- Westblad, P, et al.
(författare)
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Differences in ankle-joint complex motion during the stance phase of walking as measured by superficial and bone-anchored markers
- 2002
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Ingår i: Foot & ankle international. - : SAGE Publications. - 1071-1007 .- 1944-7876. ; 23:9, s. 856-863
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Tidskriftsartikel (refereegranskat)abstract
- Bones of the ankle-joint complex are difficult to represent with superficial markers and an invasive approach is required to quantify skin movement artifact. Three-dimensional coordinates during gait were first calculated from sets of three superficial markers located on both the lower leg and heel. Intracortical pins equipped with external marker arrays were subsequently inserted in the tibia, talus and calcaneus for further trials. Tibiocalcaneal and talocalcaneal joint helical axis component rotations were calculated. Intersubject motion patterns were comparable. Root mean square (RMS) differences were chosen to describe discrepancies between temporal intracortical pin and superficial marker curves. Results for the tibiocalcaneal rotations inversion / eversion, plantarflexion / dorsiflexion and abduction / adduction were 2.5°, 1.7° and 2.8° respectively. Inversion / eversion about the talocalcaneal joint showed an RMS difference of 2.1°. A systematic over- or underestimation of superficial relative to bone-anchored markers could not be determined.
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