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- Hasslöf, Pamela, et al.
(författare)
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Caries prevalence in children with cleft lip and palate : a systematic review of case-control studies
- 2007
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Ingår i: International Journal of Paediatric Dentistry. - Oxford : Blackwell Publishing. - 0960-7439 .- 1365-263X. ; 17:5, s. 313-319
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Tidskriftsartikel (refereegranskat)abstract
- AIM: To conduct a systematic review of literature in order to examine the evidence of an increased prevalence of dental caries in children with cleft lip and palate (CLP).METHODS: A search of the PubMed database was conducted through May 2006. Sex- and age-matched case-control studies with noncavitated and manifest caries lesions as endpoint were targeted (n = 6). The studies were assessed independently by two reviewers and scored A-C according to predetermined criteria for methodology and performance.RESULTS: Significantly more caries in CLP children were reported in two of the four studies in the permanent dentition and in three out of four publications dealing with primary teeth. None of the articles were, however, assessed with the highest grade 'A' and the level of evidence was therefore based on three papers graded 'B'. There was a tendency towards higher caries scores in preschool children, but as conflicting results were revealed, the evidence that children with CLP exhibit more caries than noncleft controls was inconclusive.CONCLUSION: This systematic review of literature was unable to find firm evidence for the assumption that CLP children have an increased prevalence of dental caries.
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| 3. |
- Hedberg, Maria, et al.
(författare)
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Sugar fermentation in probiotic bacteria : an in vitro study
- 2008
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Ingår i: Oral Microbiology and Immunology. - 0902-0055 .- 1399-302X. ; 23:6, s. 482-485
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Tidskriftsartikel (refereegranskat)abstract
- INTRODUCTION: Food supplemented with probiotic bacteria is a rapidly growing sector of the market. The aim of the present study was to evaluate and compare the acid production of selected probiotic strains available in commercial products.METHODS: Six Lactobacillus strains (Lactobacillus plantarum 299v and 931; Lactobacillus rhamnosus GG and LB21; Lactobacillus paracasei subsp. paracasei F19, and Lactobacillus reuteri PTA 5289) were cultivated at 37 degrees C in an anaerobic atmosphere on Man, Rogosa, Shape (MRS) agar for 48 h or MRS broth for 16 h. After centrifugation, the cells were washed and resuspended in sterile phosphate-buffered saline and immediately subjected to a fermentation assay with 12 different carbohydrates (nine sugars and three sugar alcohols) in microtiter plates with a pH indicator. The plates were examined for color changes after 24, 48, and 72 h of incubation under aerobic and anaerobic conditions. Three scores were used: negative (pH > 6.8); weak (pH 5.2-6.8), and positive (pH < 5.2). The strains were characterized with the API 50 CH system to confirm their identity.RESULTS: L. plantarum fermented all the sugars except for melibiose, raffinose, and xylitol. Both L. rhamnosus strains were generally less active although L. rhamnosus GG was slightly more active than strain LB21 in the 5% CO(2) setting. The latter strain exhibited negative reactions for sucrose, maltose, arabinose, and sorbitol under anaerobic conditions. The assays with L. paracasei and L. reuteri had negative or weak reactions for all tested sugars under both aerobic and anaerobic conditions.CONCLUSION: The metabolic capacity to form acid from dietary sugars differed significantly between the various probiotic strains.
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