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Platelet EVs contai...
Platelet EVs contain an active proteasome involved in protein processing for antigen presentation via MHC-I molecules
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- Marcoux, Genevieve (författare)
- Laval University
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- Laroche, Audrée (författare)
- Laval University
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- Hasse, Stephan (författare)
- Laval University
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visa fler...
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Bellio, Marie (författare)
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Mbarik, Maroua (författare)
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- Tamagne, Marie (författare)
- University Paris-Est Créteil
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Allaeys, Isabelle (författare)
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- Zufferey, Anne (författare)
- Laval University
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Lévesque, Tania (författare)
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- Rebetz, Johan (författare)
- Lund University,Lunds universitet,Avdelningen för hematologi och transfusionsmedicin,Institutionen för laboratoriemedicin,Medicinska fakulteten,Trombocyt immunologi,Forskargrupper vid Lunds universitet,Division of Hematology and Transfusion Medicine,Department of Laboratory Medicine,Faculty of Medicine,Platelet Immunology,Lund University Research Groups
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- Karakeussian-Rimbaud, Annie (författare)
- University Of Montreal Health Center (CRCHUM)
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- Turgeon, Julie (författare)
- University Of Montreal Health Center (CRCHUM)
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Bourgoin, Sylvain G (författare)
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- Hamzeh-Cognasse, Hind (författare)
- University of Lyon
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- Cognasse, Fabrice (författare)
- University of Lyon
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- Kapur, Rick (författare)
- University of Amsterdam
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- Semple, John W (författare)
- Lund University,Lunds universitet,Avdelningen för hematologi och transfusionsmedicin,Institutionen för laboratoriemedicin,Medicinska fakulteten,Trombocyt immunologi,Forskargrupper vid Lunds universitet,Division of Hematology and Transfusion Medicine,Department of Laboratory Medicine,Faculty of Medicine,Platelet Immunology,Lund University Research Groups,University of Toronto
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- Hébert, Marie-Josée (författare)
- University Of Montreal Health Center (CRCHUM)
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- Pirenne, France (författare)
- University Paris-Est Créteil
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- Overkleeft, Herman S (författare)
- Leiden University
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- Florea, Bogdan I (författare)
- Leiden University
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Dieude, Mélanie (författare)
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- Vingert, Benoît (författare)
- University Paris-Est Créteil
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- Boilard, Eric (författare)
- Laval University
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(creator_code:org_t)
- American Society of Hematology, 2021
- 2021
- Engelska.
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Ingår i: Blood. - : American Society of Hematology. - 1528-0020 .- 0006-4971. ; 138:25, s. 2607-2620
- Relaterad länk:
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http://dx.doi.org/10...
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https://scholarlypub...
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https://lup.lub.lu.s...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- In addition to their hemostatic role, platelets play a significant role in immunity. Once activated, platelets release extracellular vesicles (EVs) formed by the budding of their cytoplasmic membranes. Because of their heterogeneity, platelet EVs (PEVs) are thought to perform diverse functions. It is unknown, however, whether the proteasome is transferred from platelets to PEVs or whether its function is retained. We hypothesized that functional protein processing and antigen presentation machinery are transferred to PEVs by activated platelets. Using molecular and functional assays, we found that the active 20S proteasome was enriched in PEVs, along with major histocompatibility complex class I (MHC-I) and lymphocyte costimulatory molecules (CD40L and OX40L). Proteasome-containing PEVs were identified in healthy donor blood, but did not increase in platelet concentrates that caused adverse transfusion reactions. They were augmented, however, after immune complex injections in mice. The complete biodistribution of murine PEVs after injection into mice revealed that they principally reached lymphoid organs, such as spleen and lymph nodes, in addition to the bone marrow, and to a lesser extent, liver and lungs. The PEV proteasome processed exogenous ovalbumin (OVA) and loaded its antigenic peptide onto MHC-I molecules, which promoted OVA-specific CD8+ T-lymphocyte proliferation. These results suggest that PEVs contribute to adaptive immunity through cross-presentation of antigens and have privileged access to immune cells through the lymphatic system, a tissue location that is inaccessible to platelets.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Hematologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Hematology (hsv//eng)
Nyckelord
- Animals
- Antigen Presentation
- Blood Platelets/chemistry
- Extracellular Vesicles/chemistry
- Histocompatibility Antigens Class I/analysis
- Humans
- Mice
- Mice, Inbred C57BL
- Proteasome Endopeptidase Complex/analysis
Publikations- och innehållstyp
- art (ämneskategori)
- ref (ämneskategori)
Hitta via bibliotek
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Blood
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- Av författaren/redakt...
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Marcoux, Genevie ...
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Laroche, Audrée
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Hasse, Stephan
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Bellio, Marie
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Mbarik, Maroua
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Tamagne, Marie
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visa fler...
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Allaeys, Isabell ...
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Zufferey, Anne
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Lévesque, Tania
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Rebetz, Johan
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Karakeussian-Rim ...
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Turgeon, Julie
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Bourgoin, Sylvai ...
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Hamzeh-Cognasse, ...
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Cognasse, Fabric ...
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Kapur, Rick
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Semple, John W
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Hébert, Marie-Jo ...
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Pirenne, France
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Overkleeft, Herm ...
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Florea, Bogdan I
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Dieude, Mélanie
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Vingert, Benoît
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Boilard, Eric
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- Om ämnet
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- MEDICIN OCH HÄLSOVETENSKAP
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MEDICIN OCH HÄLS ...
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och Klinisk medicin
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och Hematologi
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Blood
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Lunds universitet