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- Eriksson, Anders, et al.
(författare)
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Demonstration of functionally different interactions between phospholipase C-gamma and the two types of platelet-derived growth factor receptors
- 1995
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Ingår i: Journal of Biological Chemistry. - : American Society for Biochemistry and Molecular Biology. - 0021-9258 .- 1083-351X. ; 270:13, s. 7773-7781
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Tidskriftsartikel (refereegranskat)abstract
- Phosphorylated tyrosine residues in receptor tyrosine kinases serve as binding sites for signal transduction molecules. We have identified two autophosphorylation sites, Tyr-988 and Tyr-1018, in the platelet-derived growth factor (PDGF) alpha-receptor carboxyl-terminal tail, which are involved in binding of phospholipase C-gamma (PLC-gamma). The capacities of the Y988F and Y1018F mutant PDGF alpha-receptors, expressed in porcine aortic endothelial cells, to bind PLC-gamma are 60 and 5% of that of the wild-type receptor, respectively. Phosphorylated but not unphosphorylated peptides containing Tyr-1018 are able to compete with the intact receptor for binding to immobilized PLC-gamma SH2 domains; a phosphorylated Tyr-988 peptide competes 10 times less efficiently. The complex between PLC-gamma and the PDGF alpha-receptor is more stable than that of PLC-gamma and the PDGF beta-receptor. However, PDGF stimulation results in a smaller fraction of tyrosine-phosphorylated PLC-gamma and a smaller accumulation of inositol trisphosphate in cells expressing the alpha-receptor as compared with cells expressing the beta-receptor. We conclude that phosphorylated Tyr-988 and Tyr-1018 in the PDGF alpha-receptor carboxyl-terminal tail bind PLC-gamma, but this association leads to only a relatively low level of tyrosine phosphorylation and activation of PLC-gamma.
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| 2. |
- Hellman, Eva
(författare)
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Darshan. Det gudomliga skådandet
- 1997
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Ingår i: Svensk religionshistorisk årsbok. - 0283-0302. ; , s. 207-209
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Recension (refereegranskat)
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| 10. |
- Hellman, Eva
(författare)
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Kvinder i det offentlige rum
- 1999
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Ingår i: Når tro bliver til ret. - : Kvindernes u-landsudvalg: Köpenhamn. - 8787618435 ; , s. 16-19
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Bokkapitel (populärvet., debatt m.m.)
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