| 1. |
- Ahlberg, Viktor, et al.
(författare)
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Innate immune responses induced by the saponin adjuvant Matrix-M in specific pathogen free pigs
- 2017
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Ingår i: Veterinary Research. - : Springer Science and Business Media LLC. - 0928-4249 .- 1297-9716. ; 48
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Tidskriftsartikel (refereegranskat)abstract
- Saponin-based adjuvants have been widely used to enhance humoral and cellular immune responses in many species, but their mode of action is not fully understood. A characterization of the porcine transcriptional response to Matrix-M was performed in vitro using lymphocytes, monocytes or monocyte-derived dendritic cells (MoDCs) and in vivo. The effect of Matrix-M was also evaluated in specific pathogen free (SPF) pigs exposed to conventionally reared pigs. The pro-inflammatory cytokine genes IL1B and CXCL8 were up-regulated in monocytes and lymphocytes after Matrix-M exposure. Matrix-M also induced IL12B, IL17A and IFNG in lymphocytes and IFN-a gene expression in MoDCs. Several genes were indicated as up-regulated by Matrix-M in blood 18 h after injection, of which the genes for IFN-a and TLR2 could be statistically confirmed. Respiratory disease developed in all SPF pigs mixed with conventional pigs within 1-3 days. Two out of four SPF pigs injected with saline prior to contact exposure displayed systemic symptoms that was not recorded for the four pigs administered Matrix-M. Granulocyte counts, serum amyloid A levels and transcription of IL18 and TLR2 coincided with disease progression in the pigs. These results support further evaluation of Matrix-M as a possible enhancer of innate immune responses during critical moments in pig management.
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| 2. |
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| 3. |
- Hellman, Stina, et al.
(författare)
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The adjuvant G3 promotes a Th1 polarizing innate immune response in equine PBMC
- 2018
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Ingår i: Veterinary Research. - : Springer Science and Business Media LLC. - 0928-4249 .- 1297-9716. ; 49
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Tidskriftsartikel (refereegranskat)abstract
- The immunomodulatory effect of a new particulate adjuvant, G3, alone or in combination with agonists to TLR2/1 or TLR5 was evaluated in cultures of equine PBMC. Exposure to the G3 adjuvant up-regulated genes encoding IFN-, IL-1, IL-6, IL-8, IL-12p40 and IL-23p19 in the majority of the horses tested, indicating that the G3 adjuvant induced a pro-inflammatory and Th1 dominated profile. In accordance, genes encoding IL-13, IL-4, IL-10 and TGF- remained unaffected and genes encoding IFN-, IL-17A and TNF- were only occasionally and weakly induced. The two TLR agonists Pam3CSK4 (TLR2/1) and FliC (TLR5) induced cytokine profiles characterized by a clear induction of IL-10 as well as up-regulation of the genes encoding IL-1, IL-6 and IL-8. The presence of G3 modified this response, in particular by reducing the FliC and Pam3CSK4 induced production of IL-10. Furthermore, G3 acted in synergy with Pam3CSK4 in enhancing the production of IFN- whereas G3 combined with FliC increased the gene expression of IL-8. Thus, the G3 adjuvant seems to have the capacity to promote a Th1 polarizing innate immune response in eqPBMC, both by favouring IFN- production and by reducing production of IL-10 induced by co-delivered molecules. These features make G3 an interesting candidate to further evaluate for its potential as an adjuvant in equine vaccines.
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| 4. |
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| 5. |
- Rosengren, Björn, et al.
(författare)
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EPA – en modell för att träna och bedöma dagligt läkarjobb
- 2019
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Ingår i: Läkartidningen. - : Läkartidningen Förlag AB. - 0023-7205 .- 1652-7518. ; 116:19-20
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Tidskriftsartikel (övrigt vetenskapligt/konstnärligt)abstract
- Entrustable Professional Activities (EPAs) define observable key tasks that a doctor should be able to do independently after having completed a certain level of education. Progression is facilitated by frequent assessments of the defined activities, with increased degree of independence. The observations should be supported by constructive feed-forward. EPA is currently used in several countries both in undergraduate and post graduate education. In the context of pending changes in Swedish legislation regarding undergraduate education qualifications ordinance and medical licensing, a Swedish national work group has begun to define Swedish EPA for the future undergraduate education.
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| 6. |
- Rosengren, Björn, et al.
(författare)
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EPA - en modell för att träna och bedöma dagligt läkarjobb : [EPA (Entrustable professional activities) - an international approach to define key tasks that a doctor should be able to perform]
- 2019
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Ingår i: Läkartidningen. - : Sveriges läkarförbund. - 0023-7205 .- 1652-7518. ; 116:19-20, s. 872-874
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Tidskriftsartikel (refereegranskat)abstract
- Entrustable Professional Activities (EPAs) define observable key tasks that a doctor should be able to do independently after having completed a certain level of education. Progression is facilitated by frequent assessments of the defined activities, with increased degree of independence. The observations should be supported by constructive feed-forward. EPA is currently used in several countries both in undergraduate and post graduate education. In the context of pending changes in Swedish legislation regarding undergraduate education qualifications ordinance and medical licensing, a Swedish national work group has begun to define Swedish EPA for the future undergraduate education.
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| 7. |
- Rosengren, Björn, et al.
(författare)
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[EPA (Entrustable professional activities) - an international approach to define key tasks that a doctor should be able to perform]. : EPA – en modell för att träna och bedöma dagligt läkarjobb.
- 2019
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Ingår i: Lakartidningen. - 1652-7518. ; 116
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Tidskriftsartikel (refereegranskat)abstract
- Entrustable Professional Activities (EPAs) define observable key tasks that a doctor should be able to do independently after having completed a certain level of education. Progression is facilitated by frequent assessments of the defined activities, with increased degree of independence. The observations should be supported by constructive feed-forward. EPA is currently used in several countries both in undergraduate and post graduate education. In the context of pending changes in Swedish legislation regarding undergraduate education qualifications ordinance and medical licensing, a Swedish national work group has begun to define Swedish EPA for the future undergraduate education.
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| 8. |
- Rosengren, Björn, et al.
(författare)
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Tio »väsentliga aktiviteter« framtagna för läkarprogrammen
- 2019
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Ingår i: Läkartidningen. - 0023-7205. ; 47:19
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Tidskriftsartikel (övrigt vetenskapligt/konstnärligt)abstract
- De 10 EPA som presenteras här (Fakta 1) har tagits fram av läkare och studenter som representerar läkarutbildningarna vid samtliga lärosäten. Utvecklingsarbetet baserades på internationell litteratur och förslagen har genomgått valideringsprocesser [2]. Samtliga avser aktiviteter som ingår i en läkares dagliga arbete och som läkaren ska klara självständigt (i förhållande till handledare) första dagen som legitimerad läkare. Det är samtidigt viktigt att poängtera att de bara täcker in en del av examensmålen för läkare.
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