| 1. |
- Björk, Per, et al.
(författare)
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Biomolecular nanowires decorated by organic electronic polymers
- 2010
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Ingår i: JOURNAL OF MATERIALS CHEMISTRY. - : Royal Society of Chemistry (RSC). - 0959-9428 .- 1364-5501. ; 20:12, s. 2269-2276
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Tidskriftsartikel (refereegranskat)abstract
- We demonstrate the shaping and forming of organic electronic polymers into designer nanostructures using biomacromolecules. In order to create nanowires, nanohelixes and assemblies of these, we coordinate semiconducting or metallic polymers to biomolecular polymers in the form of DNA and misfolded proteins. Optoelectronic and electrochemical devices utilizing these shaped materials are discussed.
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| 2. |
- Hamedi, Mahiar, et al.
(författare)
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Nanocellulose Aerogels Functionalized by Rapid Layer-by-Layer Assembly for High Charge Storage and Beyond
- 2013
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Ingår i: Angewandte Chemie International Edition. - : Wiley. - 1433-7851 .- 1521-3773. ; 52:46, s. 12038-12042
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Tidskriftsartikel (refereegranskat)abstract
- Step by step: A robust and rapid method for the layer-by-layer assembly of polymers and nanoparticles on strong and elastic aerogels has been developed. Thin films of biomolecules, conducting polymers, and carbon nanotubes were assembled, which resulted in aerogels with a number of functions, including a high charge-storage capacity.
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| 3. |
- Herland, Anna, et al.
(författare)
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Electrochemical Control of Growth Factor Presentation To Steer Neural Stem Cell Differentiation
- 2011
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Ingår i: Angewandte Chemie International Edition. - Weinheim : Wiley-VCH Verlagsgesellschaft. - 1433-7851 .- 1521-3773. ; 50:52, s. 12529-12533
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Tidskriftsartikel (refereegranskat)abstract
- Graphical Abstract Let it grow: The conjugated polymer poly(3,4-ethylenedioxythiophene) (PEDOT) was synthesized with heparin as the counterion to form a cell culture substrate. The surface of PEDOT:heparin in the neutral state associated biologically active growth factors (see picture). Electrochemical in situ oxidation of PEDOT during live cell culture decreased the bioavailability of the growth factor and created an exact onset of neural stem cell differentiation.
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| 4. |
- Lundin, Vanessa, et al.
(författare)
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Control of Neural Stem Cell Survival by Electroactive Polymer Substrates
- 2011
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Ingår i: PLOS ONE. - : Public Library of Science (PLoS). - 1932-6203. ; 6:4, s. 0018624-
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Tidskriftsartikel (refereegranskat)abstract
- Stem cell function is regulated by intrinsic as well as microenvironmental factors, including chemical and mechanical signals. Conducting polymer-based cell culture substrates provide a powerful tool to control both chemical and physical stimuli sensed by stem cells. Here we show that polypyrrole (PPy), a commonly used conducting polymer, can be tailored to modulate survival and maintenance of rat fetal neural stem cells (NSCs). NSCs cultured on PPy substrates containing different counter ions, dodecylbenzenesulfonate (DBS), tosylate (TsO), perchlorate (ClO4) and chloride (Cl), showed a distinct correlation between PPy counter ion and cell viability. Specifically, NSC viability was high on PPy(DBS) but low on PPy containing TsO, ClO4 and Cl. On PPy(DBS), NSC proliferation and differentiation was comparable to standard NSC culture on tissue culture polystyrene. Electrical reduction of PPy(DBS) created a switch for neural stem cell viability, with widespread cell death upon polymer reduction. Coating the PPy(DBS) films with a gel layer composed of a basement membrane matrix efficiently prevented loss of cell viability upon polymer reduction. Here we have defined conditions for the biocompatibility of PPy substrates with NSC culture, critical for the development of devices based on conducting polymers interfacing with NSCs.
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| 5. |
- Shaw, Alan, et al.
(författare)
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Spatial control of membrane receptor function using ligand nanocalipers
- 2014
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Ingår i: Nature Methods. - : Springer Nature. - 1548-7091 .- 1548-7105. ; 11:8, s. 841-846
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Tidskriftsartikel (refereegranskat)abstract
- The spatial organization of membrane-bound ligands is thought to regulate receptor-mediated signaling. However, direct regulation of receptor function by nanoscale distribution of ligands has not yet been demonstrated, to our knowledge. We developed rationally designed DNA origami nanostructures modified with ligands at well-defined positions. Using these 'nanocalipers' to present ephrin ligands, we showed that the nanoscale spacing of ephrin-A5 directs the levels of EphA2 receptor activation in human breast cancer cells. Furthermore, we found that the nanoscale distribution of ephrin-A5 regulates the invasive properties of breast cancer cells. Our ligand nanocaliper approach has the potential to provide insight into the roles of ligand nanoscale spatial distribution in membrane receptor mediated signaling.
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