Sökning: WFRF:(Hermanson Monica)
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Assessing hematopoi...
Assessing hematopoietic chimerism after stem cell transplantation by multiplexed SNP genotyping using microarrays and quantitative analysis of SNP alleles
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- Fredriksson, Mona (författare)
- Uppsala universitet,Institutionen för medicinska vetenskaper
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- Barbany, Gisela (författare)
- Uppsala universitet,Institutionen för genetik och patologi
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- Liljedahl, Ulrika (författare)
- Uppsala universitet,Molekylär medicin
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- Hermanson, Monica (författare)
- Uppsala universitet,Medicinsk genetik,Institutionen för genetik och patologi
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Kataja, Matti (författare)
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- Syvänen, Ann-Christine (författare)
- Uppsala universitet,Molekylär medicin
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(creator_code:org_t)
- 2003-12-04
- 2004
- Engelska.
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Ingår i: Leukemia. - : Springer Science and Business Media LLC. - 0887-6924 .- 1476-5551. ; 18:2, s. 255-266
- Relaterad länk:
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https://www.nature.c...
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https://urn.kb.se/re...
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https://doi.org/10.1...
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https://urn.kb.se/re...
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Abstract
Ämnesord
Stäng
- Single-nucleotide polymorphisms (SNPs) have the potential to be particularly useful as markers for monitoring of chimerism after stem cell transplantation (SCT) because they can be analyzed by accurate and robust methods. We used a two-phased minisequencing strategy for monitoring chimerism after SCT. First, informative SNPs with alleles differing between donor and recipient were identified using a multiplex microarray-based minisequencing system screening 51 SNPs to ensure that multiple informative SNPs were detected in each donor-recipient pair. Secondly, the development of chimerism was followed up after SCT by sensitive, quantitative analysis of individual informative SNPs by applying the minisequencing method in a microtiter plate format. Using this panel of SNPs, we identified multiple informative SNPs in nine unrelated and in 16 related donor-recipient pairs. Samples from nine of the donor-recipient pairs taken at time points ranging from 1 month to 8 years after transplantation were available for analysis. In these samples, we monitored the allelic ratios of two or three informative SNPs in individual minisequencing reactions. The results agreed well with the data obtained by microsatellite analysis. Thus, we conclude that the two-phased minisequencing strategy is a useful approach in the following up of patients after SCT.
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- art (ämneskategori)
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Leukemia
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