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| 2. |
- Karlsson, J, 1966-, et al.
(författare)
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Sequencing of the Francisella tularensis strain Schu 4 genome reveals the shikimate and purine metabolic pathways, targets for the construction of a rationally attenuated auxotrophic vaccine.
- 2000
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Ingår i: Microbial & Comparative Genomics. - : Mary Ann Liebert Inc. - 1090-6592 .- 2168-6637. ; 5:1, s. 25-39
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Tidskriftsartikel (refereegranskat)abstract
- Francisella tularensis is the etiological agent of tularemia, a serious disease in several Northern hemisphere countries. The organism has fastidious growth requirements and is very poorly understood at the genetic and molecular levels. Given the lack of data on this organism, we undertook the sample sequencing of its genome. A random library of DNA fragments from a highly virulent strain (Schu 4) of F. tularensis was constructed and the nucleotide sequences of 13,904 cloned fragments were determined and assembled into 353 contigs. A total of 1.83 Mb of nucleotide sequence was obtained that had a G+C content of 33.2%. Genes located on plasmids pOM1 and pNFL10, which had been previously isolated from low virulence strains of F. tularensis, were absent but all of the other known F. tularensis genes were represented in the assembled data. F. tularensis Schu4 was able to grow in the absence of aromatic amino acids and orthologues of genes which could encode enzymes in the shikimate pathway in other bacteria were identified in the assembled data. Genes that could encode all of the enzymes in the purine biosynthetic and most of the en- zymes in the purine salvage pathways were also identified. This data will be used to develop defined rationally attenuated mutants of F. tularensis, which could be used as replacements for the existing genetically undefined live vaccine strain.
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| 3. |
- From Attebring, M, et al.
(författare)
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Are patients truthfull about their smoking habits? A validation of self-report about smoking cessation with biochemical markers of smoking activity amongst patients with ischaemic heart disease.
- 2001
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Ingår i: Journal of Internal Medicine. - : Wiley-Blackwell Publishing Ltd. - 0954-6820 .- 1365-2796. ; 249:2, s. 145-151
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Tidskriftsartikel (refereegranskat)abstract
- AIMS: To validate self-report about smoking cessation with biochemical markers of smoking activity amongst patients with ischaemic heart disease. PATIENTS AND METHODS: Outpatients at the Division of Cardiology, 75 years of age or younger, who had been Hospitalized at Sahlgrenska University Hospital in Göteborg due to an ischaemic event and who consecutively participated in a nurse-monitored routine care programme for secondary prevention, from 6 February 1997 to 5 February 1998. Data concerning smoking habits were collected through interviews. Two chemical markers, cotinine in plasma and carbon monoxide (CO) in expired air, validated self-reports concerning smoking cessation. RESULTS: 260 former smokers were validated. In the vast majority of the study population, the anamnestic information concurred with the chemical marker. However, 17 patients had chemical markers that contradicted their self-report with raised CO (n = 6) and/or raised cotinine levels (n = 13) without alternative nicotine delivery. CONCLUSION: Most patients with coronary artery disease relating information concerning cessation of smoking are truthful. A few patients, however, seem to conceal their smoking. Testing by chemical markers may be questionable for ordinary care but should, however, be included in studies concerning the association between smoking and health.
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| 4. |
- Blomgren, J, et al.
(författare)
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Laboratory examination - does it work?
- 2001
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Ingår i: Conference on quality and developement work at univerities and colleges: Norrköping, Sweden, september 25-27, 2001.
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Konferensbidrag (populärvet., debatt m.m.)
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| 5. |
- Ciarimboli, Giuliano, et al.
(författare)
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Dynamic alterations of glomerular charge density in fixed rat kidneys suggest involvement of endothelial cell coat
- 2003
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Ingår i: Am J Physiol Renal Physiol.. ; 285:4
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Tidskriftsartikel (refereegranskat)abstract
- In a previous paper, we found that low ionic strength (I) reversibly reduced the glomerular charge density, suggesting increased volume of the charge-selective barrier. Because glutaraldehyde makes most structures rigid, we considered the isolated, perfusion-fixed rat kidney to be an ideal model for further analysis. The fixed kidneys were perfused with albumin solutions containing FITC-Ficoll at two different Is (I = 151 and 34 mM). At normal I, the fractional clearance () for albumin was 0.0049 (SE -0.0017, +0.0027, n = 6), whereas for neutral Ficoll35.5A of similar size was significantly higher 0.104 (SE 0.010, n = 5, P < 0.001). At low I, for albumin was 0.0030 (SE -0.0011, +0.0018, n = 6, not significant from albumin at normal I) and for Ficoll35.5A was identical to that at normal I, 0.104 (SE 0.015, n = 6, P < 0.01 compared with albumin at low I). According to a heterogeneous charged fiber model, low I reduced the fiber density from 0.056 to 0.0315, suggesting a 78% gel volume expansion. We conclude that 1) there is a significant glomerular charge barrier. 2) Solutions with low I increase the volume of the charge barrier even in kidneys fixed with glutaraldehyde. Our findings suggest that polysaccharide-rich structures, such as the endothelial cell coat, are key components in the glomerular barrier.
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| 9. |
- Henriksson, H, et al.
(författare)
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Neutron emission study of DT plasmas heated with tritium neutral beams
- 2001
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Ingår i: REVIEW OF SCIENTIFIC INSTRUMENTS. - : AMER INST PHYSICS. - 0034-6748. ; 72:1, s. 832-835
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Tidskriftsartikel (refereegranskat)abstract
- The neutron emission energy spectrum from deuterium-tritium fusion reactions has been measured in experiments carried out at the Joint European Torus for plasmas heated with high power neutral beam (NB) injection of tritium beams at E-b approximate to 150
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| 10. |
- Hjalmarsson, A, et al.
(författare)
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Neutron time-of-flight spectrometer for high rate diagnosis of deuterium plasmas
- 2001
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Ingår i: REVIEW OF SCIENTIFIC INSTRUMENTS. - : AMER INST PHYSICS. - 0034-6748. ; 72:1, s. 841-844
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Tidskriftsartikel (refereegranskat)abstract
- The new concept for the design of a neutron time of flight spectrometer operating at high rates of events has been studied based on instrumental modeling and neutron transport calculations. The aim is to find an optimized design for an instrument that can
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