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- Cao, Y., et al.
(författare)
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Universal intracellular biomolecule delivery with precise dosage control
- 2018
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Ingår i: Science Advances. - : American Association for the Advancement of Science (AAAS). - 2375-2548. ; 4:10
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Tidskriftsartikel (refereegranskat)abstract
- Intracellular delivery of mRNA, DNA, and other large macromolecules into cells plays an essential role in an array of biological research and clinical therapies. However, currentmethods yield a wide variation in the amount of material delivered, as well as limitations on the cell types and cargoes possible. Here, we demonstrate quantitatively controlled delivery into a range of primary cells and cell lines with a tight dosage distribution using a nanostrawelectroporation system (NES). In NES, cells are cultured onto track-etched membranes with protruding nanostraws that connect to the fluidic environment beneath the membrane. The tight cell-nanostraw interface focuses applied electric fields to the cell membrane, enabling low-voltage and nondamaging local poration of the cell membrane. Concurrently, the field electrophoretically injects biomolecular cargoes through the nanostraws and into the cell at the same location. We show that the amount of material delivered is precisely controlled by the applied voltage, delivery duration, and reagent concentration. NES is highly effective even for primary cell types or different cell densities, is largely cargo agnostic, and can simultaneously deliver specific ratios of different molecules. Using a simple cell culture well format, the NES delivers into >100,000 cells within 20 s with >95% cell viability, enabling facile, dosage-controlled intracellular delivery for a wide variety of biological applications.
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| 2. |
- Hjort, Line, et al.
(författare)
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FOETAL for NCD-FOetal Exposure and Epidemiological Transitions : the role of Anaemia in early Life for Non-Communicable Diseases in later life: a prospective preconception study in rural Tanzania
- 2019
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Ingår i: BMJ Open. - : BMJ. - 2044-6055. ; 9:5
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Tidskriftsartikel (refereegranskat)abstract
- PURPOSE: Low-income and middle-income countries such as Tanzania experience a high prevalence of non-communicable diseases (NCDs), including anaemia. Studying if and how anaemia affects growth, placenta development, epigenetic patterns and newborns' risk of NCDs may provide approaches to prevent NCDs.PARTICIPANTS: The FOETALforNCD (FOetal Exposure and Epidemiological Transitions: the role of Anaemia in early Life for Non-Communicable Diseases in later life) Study is a population-based preconception, pregnancy and birth cohort study (n=1415, n=538, n=427, respectively), conducted in a rural region of North-East Tanzania. All participants were recruited prior to conception or early in pregnancy and followed throughout pregnancy as well as at birth. Data collection included: maternal blood, screening for NCDs and malaria, ultrasound in each trimester, neonatal anthropometry at birth and at 1 month of age, cord blood, placental and cord biopsies for stereology and epigenetic analyses.FINDINGS TO DATE: At preconception, the average age, body mass index and blood pressure of the women were 28 years, 23 kg/m2 and 117/75 mm Hg, respectively. In total, 458 (36.7%) women had anaemia (haemoglobin Hb <12 g/dL) and 34 (3.6%) women were HIV-positive at preconception. During pregnancy 359 (66.7%) women had anaemia of which 85 (15.8%) women had moderate-to-severe anaemia (Hb ≤9 g/dL) and 33 (6.1%) women had severe anaemia (Hb ≤8 g/dL). In total, 185 (34.4%) women were diagnosed with malaria during pregnancy.FUTURE PLANS: The project will provide new knowledge on how health, even before conception, might modify the risk of developing NCDs and how to promote better health during pregnancy. The present project ended data collection 1 month after giving birth, but follow-up is continuing through regular monitoring of growth and development and health events according to the National Road Map Strategic Plan in Tanzania. This data will link fetal adverse event to childhood development, and depending on further grant allocation, through a life course follow-up.
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| 5. |
- Hultman, Jens, et al.
(författare)
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Handeln rustar för multi-kanal
- 2017
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Rapport (övrigt vetenskapligt/konstnärligt)abstract
- Handelsbranschen står inför omfattande utmaningar och möjligheter som kan – och kommer – att förändra verksamheten i grunden. En av dessa genomgripande förändringar är handelns digitalisering och dess konsekvenser för detaljhandelns styrning och utveckling av multikanalserbjudanden. Från en situation där kunder och handel primärt tänker fysisk butik, till en situation där handel via internet präglar det mesta som händer; från hur kunder handlar till hur företagen tänker strategiskt på sitt erbjudande. Det är en lång resa som långtifrån är färdig och som hänger samman med digitaliseringen av samhället i stort, big data och andra möjligheter (och utmaningar) som den tekniska utvecklingen innebär.Denna utveckling är mångfacetterad och det är viktigt att belysa den ur flera perspektiv. Centrum för handelsforsknings verksamhet är unik i den bemärkelse att vi i samtliga forskningsprogram arbetar tvärvetenskapligt där forskare från alla de discipliner som är representerade i Centrum finns med i varje forskningsprogram. Detta garanterar en mångsidig belysning av de forskningsproblem som vi tar oss an. Under 2014–2015 har vi drivit forskningsprogrammen E-handel och Kunniga kunder. Därefter startade vi programmen Retail destination och Retail innovation.I denna skrift sammanfattas forskningsresultaten från forskningsprogrammet E-handel. Programchef för E-handel har Jens Hultman varit. Deltagande forskare i programmet har varit Klas Hjort, Veronika Tarnovskaya, Ulf Johansson och Christian Fuentes. Under 2014 och 2015 (och även 2016) har resultat från programmet presenterats i olika sammanhang. De har presenterats vid forskningsseminarier och workshops som Centrum har arrangerat, de har presenterats vid vetenskapliga konferenser och vid praktikerkonferenser och de har tidigare också beskrivits i rapporter och vetenskapliga artiklar. Nu presenterar vi resultaten inom ramen för Handelsrådets rapportserie.
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| 6. |
- Webb, J. L., et al.
(författare)
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Imaging Atomic Scale Dynamics on III-V Nanowire Surfaces during Electrical Operation
- 2017
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Ingår i: Scientific Reports. - : Springer Science and Business Media LLC. - 2045-2322. ; 7:1
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Tidskriftsartikel (refereegranskat)abstract
- As semiconductor electronics keep shrinking, functionality depends on individual atomic scale surface and interface features that may change as voltages are applied. In this work we demonstrate a novel device platform that allows scanning tunneling microscopy (STM) imaging with atomic scale resolution across a device simultaneously with full electrical operation. The platform presents a significant step forward as it allows STM to be performed everywhere on the device surface and high temperature processing in reactive gases of the complete device. We demonstrate the new method through proof of principle measurements on both InAs and GaAs nanowire devices with variable biases up to 4 V. On InAs nanowires we observe a surprising removal of atomic defects and smoothing of the surface morphology under applied bias, in contrast to the expected increase in defects and electromigration-related failure. As we use only standard fabrication and scanning instrumentation our concept is widely applicable and opens up the possibility of fundamental investigations of device surface reliability as well as new electronic functionality based on restructuring during operation.
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