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- Hofius, Daniel
(författare)
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Autophagy deficiency leads to accumulation of ubiquitinated proteins, ER stress, and cell death in Arabidopsis
- 2014
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Ingår i: Autophagy. - : Informa UK Limited. - 1554-8627 .- 1554-8635. ; 10, s. 1579-1587
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Tidskriftsartikel (refereegranskat)abstract
- Autophagy is a homeostatic degradation and recycling process that is also involved in defense against microbial pathogens and in certain forms of cellular suicide. Autophagy has been proposed to negatively regulate plant immunity-associated cell death related to the hypersensitive response (HR), as older autophagy-deficient mutants are unable to contain this type of cell death 5 to 10 d after infection. Such spreading cell death was found to require NPR1 (nonexpressor of PR genes 1), but surprisingly did not occur in younger atg mutants. In contrast, we find that npr1 mutants are not impaired in rapid programmed cell death activation upon pathogen recognition. Furthermore, our molecular evidence suggests that the NPR1-dependent spreading cell death in older atg mutants may originate from an inability to cope with excessive accumulation of ubiquitinated proteins and ER stress which derive from salicylic acid (SA)-dependent signaling (e. g., systemic acquired resistance). We also demonstrate that both senescence and immunity-related cell death seen in older atg mutants can be recapitulated in younger atg mutants primed with ER stress. We therefore propose that the reduction in SA signaling caused by npr1 loss-of-function is sufficient to alleviate the stress levels accumulated during aging in autophagy deficient cells which would otherwise become insurmountable and lead to uncontrolled cell death.
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| 2. |
- Hofius, Daniel
(författare)
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Signaling unmasked
- 2014
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Ingår i: Autophagy. - : Informa UK Limited. - 1554-8627 .- 1554-8635. ; 10, s. 520-521
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Annan publikation (refereegranskat)
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| 3. |
- Kock, Teh Ooi, et al.
(författare)
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Membrane trafficking and autophagy in pathogen-triggered cell death and immunity
- 2014
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Ingår i: Journal of Experimental Botany. - : Oxford University Press (OUP). - 0022-0957 .- 1460-2431. ; 65, s. 1297-1312
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Forskningsöversikt (refereegranskat)abstract
- Plants respond to pathogen attack with dynamic rearrangements of the endomembrane system and rapid redirection of membrane traffic to facilitate effective host defence. Mounting evidence indicates the involvement of endocytic, secretory, and vacuolar trafficking pathways in immune receptor activation, signal transduction, and execution of multiple defence responses including programmed cell death (PCD). Autophagy is a conserved intracellular trafficking and degradation process and has been implicated in basal immunity as well as in some forms of immune receptor-mediated vacuolar cell death. However, the regulatory interplay of autophagy and other membrane trafficking pathways in PCD and defence responses remains obscure. This review therefore highlights recent advances in the understanding of autophagic and membrane trafficking during plant immunity, and discusses emerging molecular links and functional interconnections.
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