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- Klionsky, Daniel J., et al.
(författare)
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Guidelines for the use and interpretation of assays for monitoring autophagy
- 2012
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Ingår i: Autophagy. - : Informa UK Limited. - 1554-8635 .- 1554-8627. ; 8:4, s. 445-544
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Forskningsöversikt (refereegranskat)abstract
- In 2008 we published the first set of guidelines for standardizing research in autophagy. Since then, research on this topic has continued to accelerate, and many new scientists have entered the field. Our knowledge base and relevant new technologies have also been expanding. Accordingly, it is important to update these guidelines for monitoring autophagy in different organisms. Various reviews have described the range of assays that have been used for this purpose. Nevertheless, there continues to be confusion regarding acceptable methods to measure autophagy, especially in multicellular eukaryotes. A key point that needs to be emphasized is that there is a difference between measurements that monitor the numbers or volume of autophagic elements (e.g., autophagosomes or autolysosomes) at any stage of the autophagic process vs. those that measure flux through the autophagy pathway (i.e., the complete process); thus, a block in macroautophagy that results in autophagosome accumulation needs to be differentiated from stimuli that result in increased autophagic activity, defined as increased autophagy induction coupled with increased delivery to, and degradation within, lysosomes (in most higher eukaryotes and some protists such as Dictyostelium) or the vacuole (in plants and fungi). In other words, it is especially important that investigators new to the field understand that the appearance of more autophagosomes does not necessarily equate with more autophagy. In fact, in many cases, autophagosomes accumulate because of a block in trafficking to lysosomes without a concomitant change in autophagosome biogenesis, whereas an increase in autolysosomes may reflect a reduction in degradative activity. Here, we present a set of guidelines for the selection and interpretation of methods for use by investigators who aim to examine macroautophagy and related processes, as well as for reviewers who need to provide realistic and reasonable critiques of papers that are focused on these processes. These guidelines are not meant to be a formulaic set of rules, because the appropriate assays depend in part on the question being asked and the system being used. In addition, we emphasize that no individual assay is guaranteed to be the most appropriate one in every situation, and we strongly recommend the use of multiple assays to monitor autophagy. In these guidelines, we consider these various methods of assessing autophagy and what information can, or cannot, be obtained from them. Finally, by discussing the merits and limits of particular autophagy assays, we hope to encourage technical innovation in the field.
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| 3. |
- Abdollahpour, N., et al.
(författare)
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An improving working condition system (health, safety and ergonomics) survey and analysis with macroergonomics approach in a manufacturing company from Iran Power Plant Industry in 2010
- 2013
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Ingår i: Iran Occupational Health. - 1735-5133 .- 2228-7493. ; 10:4, s. 55-64
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Tidskriftsartikel (refereegranskat)abstract
- Background and aims: World experience has shown the positive effect of applying ergonomics in improving work conditions, of quality and quantity of production, reducing prevalence of work-related musculoskeletal disorders, saving costs and increase of productivity in the different contents. These are possible only with utilizing properly improving work condition system and Macroergonomics approach. The aim of the study was how determine situation and analyzing the working conditions using the macroergonomics approach in a Power Plant Industry. Methods: This study is a cross-sectional study. First, the program for improvement of work conditions were evaluated using three questionnaires including managers viewpoint (29 samples), health, safety and environment employees viewpoint (5 samples) and operating workers viewpoint (85 samples) and then three other instruments of macroergonomics were used. Results: According to the results of 1) questionnaires and corresponding tests, three of the program's goals of improving working conditions and promotion practices that have the same opinions, but in some cases had different opinions. 2) Future workshops and interviews, the lack of participatory leadership and lack of collaborative work systems for the company stated. 3) Apply ergonomics checklist of ILO, an appropriate interaction between the top and bottom surfaces revealed. Conclusion: Awakened needs of change in the middle and lower levels of organization to solve problems relate to improving working conditions of the system was created. To implement an Ergonomics Intervention Programme Technique Process requires the full support management and employees.
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