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Träfflista för sökning "WFRF:(Hunt J. L.) srt2:(2000-2004)"

Sökning: WFRF:(Hunt J. L.) > (2000-2004)

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1.
  • Hunt, L, et al. (författare)
  • Gene-specific expression and calcium activation of Arabidopsis thaliana phospholipase C isoforms
  • 2004
  • Ingår i: New Phytologist. - : Wiley. - 1469-8137 .- 0028-646X. ; 162:3, s. 643-654
  • Tidskriftsartikel (refereegranskat)abstract
    • PI-PLCs synthesise the calcium releasing second messenger IP3. We investigated the expression patterns of the Arabidopsis PI-PLC gene family and measured in vitro activity of encoded enzymes. Gene specific RT-PCR and promoter-GUS fusions were used to analyse AtPLC gene expression patterns. The five available AtPLC cDNAs were expressed as fusion proteins in Escherichia coli. All members of the AtPLC gene family were expressed in multiple organs of the plant. AtPLC1, and AtPLC5 expression was localized to the vascular cells of roots and leaves with AtPLC5::GUS also detected in the guard cells. AtPLC4::GUS was detected in pollen and cells of the stigma surface. In seedlings, AtPLC2 and AtPLC3 were constitutively expressed, while AtPLCs 1, 4 and 5 were induced by abiotic stresses. AtPLC1-5 were all shown to have phospholipase C activity in the presence of calcium ions. AtPLCs showed limited tissue specific expression and expression of at least three genes was increased by abiotic stress. The differing calcium sensitivities of recombinant AtPLC protein activities may provide a mechanism for generating calcium signatures.
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  • Hunt, L, et al. (författare)
  • Phospholipase C is required for the control of stomatal aperture by ABA
  • 2003
  • Ingår i: Plant Journal. - 1365-313X. ; 34:1, s. 47-55
  • Tidskriftsartikel (refereegranskat)abstract
    • The calcium-releasing second messenger inositol 1,4,5-trisphosphate is involved in the regulation of stomatal aperture by ABA. In other signalling pathways, inositol 1,4,5-trisphosphate is generated by the action of phospholipase C. We have studied the importance of phospholipase C in guard cell ABA-signalling pathways. Immunolocalisation of a calcium-activated phospholipase C confirmed the presence of phospholipase C in tobacco guard cells. Transgenic tobacco plants with considerably reduced levels of phospholipase C in their guard cells were only partially able to regulate their stomatal apertures in response to ABA. These results suggest that phospholipase C is involved in the amplification of the calcium signal responsible for reductions in stomatal aperture in response to ABA. As full ABA-induced inhibition of stomatal opening was not observed, our results support a role for the action of other calcium-releasing second messengers in the guard cell ABA-signalling pathway. It is not known whether these different calcium-releasing second messengers act in the same or parallel ABA-signalling pathways.
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  • Pichler, T, et al. (författare)
  • Proof for trivalent Sc ions in Sc-2 @ C-84 from high-energy spectroscopy
  • 2000
  • Ingår i: PHYSICAL REVIEW B. - : AMERICAN PHYSICAL SOC. - 0163-1829. ; 62:19, s. 13196-13201
  • Tidskriftsartikel (refereegranskat)abstract
    • The electronic structure and the valency of the Sc ions in the endohedral dimetallofullerene Sc-2 @ C-84 with D-2d symmetry are probed using high-energy spectroscopy. Comparison of the Sc 2p --> 3d x-ray-absorption spectrum with calculated ionic multiplet
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  • Resultat 1-6 av 6

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