Patterning and Controlled Adhesion of Cells and Lipid Nanotube Vesicle Networks by Microfabricated Substrates

• Networks of nanotubes and vesicles offer a platform for construction of nanofluidic devicesoperating on single molecule and particle level. Here one has the opportunity to study chemistryin confined biomimetic compartments in an environment as close to nature as possible withoutgoing in-vivo.The development of lipid vesicle networks and the techniques involved has been an ongoingprocess for over 10 years. Over the years weve expanded our abilities to observe, handle, andpredict nanotube vesicle networks. Understanding of the physical properties of these systems isimperative to the explanation of the observed behavior in the conducted experiments. But werequickly approaching the limit where we also require knowledge of how these systems act andreact to their environment. If we want to use these systems for anything more than just as cooltoy we need to get a grip on the hard physical aspects of how we choose to interact with thesesystems.The research in highly organized lipid vesicle networks is going into a regime where control ofsurface properties becomes a fundamental interest. Without proper attention to the substrate wewill never achieve our scientific goals, the use of these systems in a user friendly, research ormedical industry environment, where they can be used in the research of biological membranebehavior and cellular processes for the development of medical drugs.The scientific field of lipid nanotube vesicle networks has taken several major steps toward atechnique that is of use for the general scientific community. Among these are the cell patterningcovered in paper I, concerning electroporation of cells, the pipette writing principle described inpaper II, and in paper III, the expansion of vesicle networks to the third dimension.

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NATURVETENSKAP  -- Kemi -- Fysikalisk kemi (hsv//swe)

NATURAL SCIENCES  -- Chemical Sciences -- Physical Chemistry (hsv//eng)

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