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Performance Charact...
Performance Characteristics of qPCR Assays Targeting Human- and Ruminant-Associated Bacteroidetes for Microbial Source Tracking across Sixteen Countries on Six Continents
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- Reischer, G. H. (författare)
- Interuniversity Cooperation Centre for Water and Health,Technische Universität Wien,Vienna University of Technology
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- Ebdon, J. E. (författare)
- University of Brighton
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- Bauer, J. M. (författare)
- Technische Universität Wien,Vienna University of Technology
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- Schuster, N. (författare)
- Technische Universität Wien,Vienna University of Technology
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- Ahmed, W. (författare)
- Commonwealth Scientific and Industrial Research Organisation (CSIRO)
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- Åström, Johan, 1977 (författare)
- Chalmers tekniska högskola,Chalmers University of Technology
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- Blanch, A. R. (författare)
- Universitat de Barcelona,University of Barcelona
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- Bloschl, G. (författare)
- Technische Universität Wien,Vienna University of Technology
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- Byamukama, D. (författare)
- Makerere University
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- Coakley, T. (författare)
- University of Kentucky
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Ferguson, C. (författare)
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- Goshu, G. (författare)
- Bahar Dar University
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- Ko, G. (författare)
- Seoul National University
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- Husman, A. M. D. (författare)
- Netherlands National Institute for Public Health & the Environment
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- Mushi, D. (författare)
- Sokoine University of Agriculture
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- Poma, R. (författare)
- Universidad Nacional de Salta,National University of Salta
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- Pradhan, B. (författare)
- Tribhuvan University
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- Rajal, V. (författare)
- Universidad Nacional de Salta,National University of Salta
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Schade, M. A. (författare)
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- Sommer, R. (författare)
- Interuniversity Cooperation Centre for Water and Health,Medizinische Universität Wien,Medical University of Vienna
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- Taylor, H. (författare)
- University of Brighton
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- Toth, E. M. (författare)
- Eötvös Loránd University (ELTE)
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Vrajmasu, V. (författare)
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- Wuertz, S. (författare)
- University of California
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- Mach, R. L. (författare)
- Technische Universität Wien,Vienna University of Technology
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- Farnleitner, A. H. (författare)
- Interuniversity Cooperation Centre for Water and Health,Technische Universität Wien,Vienna University of Technology
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(creator_code:org_t)
- American Chemical Society (ACS), 2013
- 2013
- Engelska.
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Ingår i: Environmental Science & Technology. - : American Chemical Society (ACS). - 0013-936X .- 1520-5851. ; 47:15, s. 8548-8556
- Relaterad länk:
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http://publications.... (primary) (free)
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- Numerous quantitative PCR assays for microbial fecal source tracking (MST) have been developed and evaluated in recent years. Widespread application has been hindered by a lack of knowledge regarding the geographical stability and hence applicability of such methods beyond the regional level. This study assessed the performance of five previously reported quantitative PCR assays targeting human-, cattle-, or ruminant-associated Bacteroidetes populations on 280 human and animal fecal samples from 16 countries across six continents. The tested cattle-associated markers were shown to be ruminant-associated. The quantitative distributions of marker concentrations in target and nontarget samples proved to be essential for the assessment of assay performance and were used to establish a new metric for quantitative source-specificity. In general, this study demonstrates that stable target populations required for marker-based MST occur around the globe. Ruminant-associated marker concentrations were strongly correlated with total intestinal Bacteroidetes populations and with each other, indicating that the detected ruminant-associated populations seem to be part of the intestinal core microbiome of ruminants worldwide. Consequently tested ruminant-targeted assays appear to be suitable quantitative MST tools beyond the regional level while the targeted human-associated populations seem to be less prevalent and stable, suggesting potential for improvements in human-targeted methods.
Ämnesord
- TEKNIK OCH TEKNOLOGIER -- Elektroteknik och elektronik (hsv//swe)
- ENGINEERING AND TECHNOLOGY -- Electrical Engineering, Electronic Engineering, Information Engineering (hsv//eng)
Nyckelord
- alters
- real-time pcr
- contamination
- sewage
- bovine fecal pollution
- quantitative pcr
- water
- evolution
- genetic-markers
- ecology
Publikations- och innehållstyp
- art (ämneskategori)
- ref (ämneskategori)
Hitta via bibliotek
Till lärosätets databas
- Av författaren/redakt...
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Reischer, G. H.
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Ebdon, J. E.
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Bauer, J. M.
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Schuster, N.
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Ahmed, W.
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Åström, Johan, 1 ...
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visa fler...
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Blanch, A. R.
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Bloschl, G.
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Byamukama, D.
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Coakley, T.
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Ferguson, C.
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Goshu, G.
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Ko, G.
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Husman, A. M. D.
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Mushi, D.
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Poma, R.
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Pradhan, B.
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Rajal, V.
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Schade, M. A.
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Sommer, R.
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Taylor, H.
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Toth, E. M.
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Vrajmasu, V.
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Wuertz, S.
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Mach, R. L.
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Farnleitner, A. ...
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visa färre...
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