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Träfflista för sökning "WFRF:(Husmark T) srt2:(1996-1999)"

Sökning: WFRF:(Husmark T) > (1996-1999)

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1.
  • Bredholt, S., et al. (författare)
  • Microbial methods for assessment of cleaning and disinfection of food-processing surfaces cleaned in a low-pressure system
  • 1999
  • Ingår i: European Food Research and Technology. - 1438-2377 .- 1438-2385. ; 209:2, s. 145-152
  • Tidskriftsartikel (refereegranskat)abstract
    • An assessment system using various microbial methods was developed for the detection of residual, surface-attached microbes and soil on surfaces after sanitation. The microbial methods tested were: conventional cultivation, microscopy using image analysis after staining with acridine orange (AO), 5-cyano-2,3-ditolyl tetrazolium chloride/4?, 6-diamidino-2-phenylindole (CTC-DAPI) and LIVE/DEAD stains, impedance measurements, viable counts with 2,3,5-triphenyltetrazolium chloride (TTC) agar and ATP measurements. A test rig was used for the trials with four different low-pressure cleaning procedures (pressure ? 20 bar flow rate 271 min-1). A strong alkaline foam cleaner, Trippel, and a mild alkaline foam cleaner, Topax 12, in combination with two disinfectants, respectively, were used. The disinfectants were peroxide-peracetic-acid-based Oxonia Aktiv and potassium-persulphate-based Virkon. Conventional cultivation combined with impedance measurements and image analysis of surfaces stained with AO, as well as CTC-DAPI, gave results that were comparable and complementary. The combination of these methods enabled a total evaluation of both the removal of biofilm and the killing of bacterial cells. The low-pressure cleaning system did not remove all of the bacterial cells from the surfaces and did not kill the bacteria even after use of the strong alkaline foam cleaner. The above-mentioned protocol carried out on the test rig can also be used to evaluate the ensitivity of microbial methods for use in certain industrial premises. © Springer-Verlag 1999.
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2.
  • Husmark, U., et al. (författare)
  • Bacillus spores and moulding with TTC agar: A useful method for the assessment of food processing equipment cleanability
  • 1999
  • Ingår i: Biofouling (Print). - 0892-7014 .- 1029-2454. ; 14:1, s. 15-24
  • Tidskriftsartikel (refereegranskat)abstract
    • In the present work a powerful and practical method for the assessment of food processing equipment cleanability is described. Surfaces were soiled with Bacillus spores. After cleaning, surfaces were overlaid with agar containing a tetrazolium salt (TTC) which stained the growing colonies red. Stained colonies could be readily detected and counted. The poorer hygienically designed areas were easily observed and contamination levels could be defined. The TTC method was compared to other classical techniques such as contact plates, direct epifluorescence microscopy (DEM) and Shapton and Hindes (SH) agar. Recovery of bacteria using the TTC method was found to be a good complement to DEM and more accurate than contact plates and SH agar. Cleanability tests on closed equipment are also reported showing the utility of the TTC method.
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Husmark, U. (3)
Rönner, Ulf (1)
Alanko, T (1)
Bredholt, S. (1)
Maukonen, J. (1)
Kujanpaa, K. (1)
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Olofson, U. (1)
Faille, C. (1)
Benezech, T. (1)
Mattila-Sandholm, T. (1)
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