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Toxicity study on C...
Toxicity study on Clinacanthus nutans leaf hexane fraction using Danio rerio embryos
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- Murugesu, Suganya (författare)
- Int Islamic Univ Malaysia, Dept Pharmaceut Chem, Kulliyyah Pharm, Pharmacognosy Res Grp, Kuantan, Pahang Darul Ma, Malaysia;Univ Kuala Lumpur, Fac Pharm & Hlth Sci, Perak Darul Ridzuan, Royal Coll Med Perak, Ipoh 30450, Perak Darul Rid, Malaysia
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- Khatib, Alfi (författare)
- Int Islamic Univ Malaysia, Dept Pharmaceut Chem, Kulliyyah Pharm, Pharmacognosy Res Grp, Kuantan, Pahang Darul Ma, Malaysia;Univ Malaysia, Int Islamic, Kulliyyah Sci, Cent Res & Anim Facil, Kuantan 25200, Pahang Darul Ma, Malaysia;Airlangga Univ, Fac Pharm, Surabaya 60155, Indonesia
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- Ahmed, Qamar Uddin (författare)
- Int Islamic Univ Malaysia, Dept Pharmaceut Chem, Kulliyyah Pharm, Pharmacognosy Res Grp, Kuantan, Pahang Darul Ma, Malaysia
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- Ibrahim, Zalikha (författare)
- Int Islamic Univ Malaysia, Dept Pharmaceut Chem, Kulliyyah Pharm, Pharmacognosy Res Grp, Kuantan, Pahang Darul Ma, Malaysia
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- Uzir, Bisha Fathamah (författare)
- Int Islamic Univ Malaysia, Dept Pharmaceut Chem, Kulliyyah Pharm, Pharmacognosy Res Grp, Kuantan, Pahang Darul Ma, Malaysia
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- Benchoula, Khaled (författare)
- Int Islamic Univ Malaysia, Dept Pharmaceut Chem, Kulliyyah Pharm, Pharmacognosy Res Grp, Kuantan, Pahang Darul Ma, Malaysia
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- Yusoff, Nik Idris Nik (författare)
- Int Islamic Univ Malaysia, Dept Pharmaceut Chem, Kulliyyah Pharm, Pharmacognosy Res Grp, Kuantan, Pahang Darul Ma, Malaysia
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- Perumal, Vikneswari (författare)
- Univ Kuala Lumpur, Fac Pharm & Hlth Sci, Perak Darul Ridzuan, Royal Coll Med Perak, Ipoh 30450, Perak Darul Rid, Malaysia
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- Alajmi, Mohamed F. (författare)
- King Saud Univ, Coll Pharm, Dept Pharmacognosy, Riyadh 11451, Saudi Arabia
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- Salamah, Sahal (författare)
- King Saud Bin Abdul Aziz Univ Hlth Sci, Jeddah 21423, Saudi Arabia
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- El-Seedi, Hesham (författare)
- Uppsala universitet,Farmakognosi,Al Rayan Coll, Al Rayan Res & Innovat Ctr, Medina 42541, Saudi Arabia;Jiangsu Univ, Int Res Ctr Food Nutr & Safety, Zhenjiang 212013, Jiangsu, Peoples R China
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(creator_code:org_t)
- ELSEVIER, 2019
- 2019
- Engelska.
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Ingår i: Toxicology reports. - : ELSEVIER. - 2214-7500. ; 6, s. 1148-1154
- Relaterad länk:
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https://doi.org/10.1...
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https://uu.diva-port... (primary) (Raw object)
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https://doi.org/10.1...
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- Clinacanthus nutans, an herbal shrub belonging to the Acanthaceae family, is traditionally used as a functional food to treat various ailments in Malaysia and Indonesia. Although the polar fraction of this plant shows nontoxic effect, the toxicity of the non-polar extract is not reported so far. The present study aimed to assess the toxic effect and determine the lethal concentration of this non-polar fraction using zebrafish embryos. The n-hexane fraction was partitioned from the crude extract of C. nutans obtained using 80% methanolic solution. After spawning of the adult male and female zebrafish, the eggs were collected, transferred into a 96-well plate and incubated with the n-hexane fraction at concentrations of 15.63 mu g/ml, 31.25 mu g/ml, 62.5 mu g/ml, 125 mu g/ml, 250 mu g/ml and 500 mu g/ml in 2% DMSO. The survival and sublethal endpoint were assessed, the mortality and hatchability rates were calculated based on microscopic observation, while the heartbeat rate was measured using DanioScope software. The median lethal concentration (LC50) of the C. nutans n-hexane fraction, which was determined using probit analysis, was calculated to be 75.49 mu g/mL, which is harmful. Moreover, gas chromatography-mass spectrometry (GC-MS) analysis revealed the presence of palmitic acid, phytol, hexadecanoic acid, 1-monopalmitin, stigmast-5-ene, pentadecanoic acid, heptadecanoic acid, 1-linolenoylglycerol and stigmasterol in the n-hexane fraction.
Ämnesord
- NATURVETENSKAP -- Geovetenskap och miljövetenskap -- Miljövetenskap (hsv//swe)
- NATURAL SCIENCES -- Earth and Related Environmental Sciences -- Environmental Sciences (hsv//eng)
Nyckelord
- Clinacanthus nutans
- Danio rerio
- DanioScope
- Median lethal concentration
- Teratogenicity
- Probit analysis
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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Murugesu, Sugany ...
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Khatib, Alfi
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Ahmed, Qamar Udd ...
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Ibrahim, Zalikha
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Uzir, Bisha Fath ...
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Benchoula, Khale ...
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Yusoff, Nik Idri ...
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Perumal, Viknesw ...
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Alajmi, Mohamed ...
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Salamah, Sahal
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El-Seedi, Hesham
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