Hyaluronan synthase in trabecular meshwork cells.

• BACKGROUND/AIMS: Hyaluronan is present in the trabecular meshwork where it is involved in the pathophysiology of aqueous outflow environment. In this study, the expression and regulation of hyaluronan synthase (HAS), which is the enzyme synthesising hyaluronan, in trabecular meshwork cells were investigated. METHODS: Cultured bovine trabecular meshwork cells (BTMCs) were used. HAS expression in BTMCs was examined by RT-PCR. The effects of transforming growth factor beta (TGF-beta) and platelet derived growth factor BB (PDGF-BB) on HAS expression in BTMCs were examined by quantitative RT-PCR. The HAS2 expression by TGF-beta and PDGF-BB at the protein level was also confirmed immunohistochemically. The production of hyaluronan from BTMCs was detected by high performance liquid chromatography (HPLC). RESULTS: Three HAS isoforms were expressed in BTMCs at the mRNA level. Among HAS isoforms, only the expression of HAS2 mRNA was increased by the administration of TGF-beta or PDGF-BB. HAS2 upregulation by these growth factors was also confirmed at the protein level. Further, hyaluronan production from BTMCs was stimulated by TGF-beta or PDGF-BB. CONCLUSION: Expression of HAS in trabecular meshwork may maintain the hyaluronan content in the aqueous outflow pathway. Its production is regulated by TGF-beta and PDGF-BB. The regulation of the expression of HAS in trabecular meshwork might be useful for modulating the aqueous outflow environment.

Nyckelord

Animals

Cattle

Cells; Cultured

Chromatography; High Pressure Liquid

Gene Expression Regulation

Glucuronosyltransferase/*analysis

Glycosyltransferases

Hyaluronic Acid/biosynthesis

Immunohistochemistry/methods

Isoenzymes/analysis

Membrane Proteins

Platelet-Derived Growth Factor/pharmacology

RNA; Messenger/analysis

Reverse Transcriptase Polymerase Chain Reaction/methods

Trabecular Meshwork/drug effects/*enzymology

Transferases

Transforming Growth Factor beta/pharmacology

Up-Regulation

Xenopus Proteins

Publikations- och innehållstyp

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