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Träfflista för sökning "WFRF:(Isaksson Johan) srt2:(2000-2004)"

Sökning: WFRF:(Isaksson Johan) > (2000-2004)

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  • Banér, Johan, et al. (författare)
  • Parallel gene analysis with allele-specific padlock probes and tag microarrays
  • 2003
  • Ingår i: Nucleic Acids Research. - 0305-1048 .- 1362-4962. ; 31:17, s. e103-
  • Tidskriftsartikel (refereegranskat)abstract
    • Parallel, highly specific analysis methods are required to take advantage of the extensive information about DNA sequence variation and of expressed sequences. We present a scalable laboratory technique suitable to analyze numerous target sequences in multiplexed assays. Sets of padlock probes were applied to analyze single nucleotide variation directly in total genomic DNA or cDNA for parallel genotyping or gene expression analysis. All reacted probes were then co-amplified and identified by hybridization to a standard tag oligonucleotide array. The technique was illustrated by analyzing normal and pathogenic variation within the Wilson disease-related ATP7B gene, both at the level of DNA and RNA, using allele-specific padlock probes.
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  • Fiedler, Markus, et al. (författare)
  • Communication requirements of generic services for Intelligent Transport Systems
  • 2004
  • Annan publikation (övrigt vetenskapligt/konstnärligt)abstract
    • In order to meet their users’ expectations and to be of efficient help for people using or maintaining Intelligent Transport Systems (ITS), related services need efficient support from the underlying communication systems, which in turn requires profound knowledge of those needs. This paper presents an approach to gather services into generic groups and a framework to classify their needs in terms of availability, performance, security and cost in a generic manner. These needs are consequently seen from the user’s perspective, but formulated in a way such that the matching towards technical parameters as e.g. throughput offered by a communication network can be performed. Upon comparing needs with offers, the feasibility of a certain communication technology for a certain type of service can be determined, which in turn helps to choose the right kind of connectivity in order to yield the desired degree of availability, performance, security and cost. This work is carried out within the VINNOVA-sponsored project PIITSA (Personal Information for Intelligent Transport Systems through Seamless communications and Autonomous decisions) conducted by Aerotech Telub, Blekinge Tekniska Högskola, Statens Provningsanstalt Borås and Vägverket, respectively.
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  • Fu, Michael, 1963, et al. (författare)
  • Myocardial hypertrophy in transgenic mice overexpressing the bovine growth hormone (bGH) gene.
  • 2000
  • Ingår i: Journal of internal medicine. - : Wiley. - 0954-6820 .- 1365-2796. ; 247:5, s. 546-52
  • Tidskriftsartikel (refereegranskat)abstract
    • OBJECTIVES: The main purpose of the present study was to characterize cardiac muscle hypertrophy using both qualitative and quantitative microscopy in mice overexpressing the bovine growth hormone. RESULTS: Measurements of 30 fibres from each group revealed that fibre diameter in transgenic hearts was significantly larger than in control hearts. There was a significant decrease in interfibrillar space in transgenic hearts as compared with control hearts. The enlarged transgenic hearts displayed unchanged organelles such as normal myofibrils and mitochondria in a normal pattern, suggesting balanced growth. Myelin structures were occasionally observed between normal myofibrils. Moreover, myocardial beta-adrenergic receptors and muscarinic receptors in the hearts of transgenic mice overproducing GH were studied to see whether they are involved in the hypertrophic process. It was shown that the density of muscarinic receptors had decreased and the super-high affinity of muscarinic receptors was lost, without any significant changes in either the density or the affinity of beta-adrenergic receptors, as compared with controls. CONCLUSIONS: These results demonstrate that a GH excess was able to induce significant myocardial hypertrophy and that there was a downregulation of muscarinic receptors.
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