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Anchorless surface ...
Anchorless surface associated glycolytic enzymes from Lactobacillus plantarum 299v bind to epithelial cells and extracellular matrix proteins
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Glenting, Jacob (författare)
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Beck, Hans Christian (författare)
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Vrang, Astrid (författare)
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Riemann, Holger (författare)
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Ravn, Peter (författare)
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Hansen, Anne Maria (författare)
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Antonsson, Martin (författare)
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- Ahrné, Siv (författare)
- Lund University,Lunds universitet,Avdelningen för livsmedel och läkemedel,Institutionen för processteknik och tillämpad biovetenskap,Institutioner vid LTH,Lunds Tekniska Högskola,Division of Food and Pharma,Department of Process and Life Science Engineering,Departments at LTH,Faculty of Engineering, LTH
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Israelsen, Hans (författare)
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Madsen, Soren (författare)
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(creator_code:org_t)
- Elsevier BV, 2013
- 2013
- Engelska.
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Ingår i: Microbiological Research. - : Elsevier BV. - 1618-0623 .- 0944-5013. ; 168:5, s. 245-253
- Relaterad länk:
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http://dx.doi.org/10...
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https://doi.org/10.1...
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https://lup.lub.lu.s...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- An important criterion for the selection of a probiotic bacterial strain is its ability to adhere to the mucosal surface. Adhesion is usually mediated by proteins or other components located on the outer cell surface of the bacterium. In the present study we characterized the adhesive properties of two classical intracellular enzymes glyceraldehyde 3-phosphate dehydrogenase (GAPDH) and enolase (ENO) isolated from the outer cell surface of the probiotic bacterium Lactobacillus plantarum 299v. None of the genes encoded signal peptides or cell surface anchoring motifs that could explain their extracellular location on the bacterial surface. The presence of the glycolytic enzymes on the outer surface was verified by western blotting using polyclonal antibodies raised against the specific enzymes. GAPDH and ENO showed a highly specific binding to plasminogen and fibronectin whereas GAPDH but not ENO showed weak binding to mucin. Furthermore, a pH dependent and specific binding of GAPDH and ENO to intestinal epithelial Caco-2 cells at pH 5 but not at pH 7 was demonstrated. The results showed that these glycolytic enzymes could play a role in the adhesion of the probiotic bacterium L. plantarum 299v to the gastrointestinal tract of the host. Finally, a number of probiotic as well non-probiotic Lactobacillus strains were analyzed for the presence of GAPDH and ENO on the outer surface, but no correlation between the extracellular location of these enzymes and the probiotic status of the applied strains was demonstrated. (c) 2013 Elsevier GmbH. All rights reserved.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Hälsovetenskap -- Näringslära (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Health Sciences -- Nutrition and Dietetics (hsv//eng)
Nyckelord
- Lactobacillus plantarum strain 299v
- Glyceraldehyde 3-phosphate
- dehydrogenase
- Enolase
- Surface enzymes
- Probiotics
Publikations- och innehållstyp
- art (ämneskategori)
- ref (ämneskategori)
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Glenting, Jacob
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Beck, Hans Chris ...
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Vrang, Astrid
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Riemann, Holger
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Ravn, Peter
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Hansen, Anne Mar ...
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visa fler...
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Antonsson, Marti ...
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Ahrné, Siv
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Israelsen, Hans
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Madsen, Soren
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visa färre...
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