| 1. |
- Brauner, S., et al.
(author)
-
Reduced expression of TRIM21/Ro52 predicts poor prognosis in diffuse large B-cell lymphoma patients with and without rheumatic disease
- 2015
-
In: Journal of Internal Medicine. - : Wiley. - 0954-6820 .- 1365-2796. ; 278:3, s. 323-332
-
Journal article (peer-reviewed)abstract
- ObjectiveTRIM21 (also known as Ro52) is an autoantigen in rheumatic disease and is predominantly expressed in leucocytes. Overexpression is associated with decreased proliferation, and the TRIM21 gene maps to a tumour suppressor locus. We therefore investigated the expression of TRIM21 in patients with diffuse large B-cell lymphoma (DLBCL) and its potential usefulness as a prognostic biomarker. Materials and methodsTRIM21 expression levels were assessed by immunohistochemistry in lymphoma biopsies from three cohorts of patients with DLBCL: 42 patients with rheumatic disease treated with a cyclophosphamide, vincristine, doxorubicin and prednisone (CHOP)-like regimen, 76 CHOP-treated and 196 rituximab-CHOP-treated nonrheumatic patients. Expression was correlated with clinical and biomedical parameters. TRIM21 expression was assessed in relation to lymphocyte proliferation by quantitative PCR and correlated with H-3-thymidine incorporation and propidium iodine staining. ResultsTRIM21 expression levels differed in the lymphomas compared to normal lymphoid tissue, with reduced expression correlating with shorter overall survival in all three cohorts. In the two larger cohorts, progression-free survival was assessed and was also found to correlate with TRIM21 expression. The association was independent of commonly used clinical prognostic scores, lymphoma subtype and several previously reported prognostic biomarkers. In agreement with this clinical observation, we noted an inverse correlation between TRIM21 expression and proliferation of leucocytes invitro. ConclusionsWe show that loss of TRIM21 expression is associated with more aggressive lymphoma and increased proliferation, whereas maintenance of TRIM21 expression is associated with better prognosis in patients with DLBCL. Based on our findings, we suggest that TRIM21 should be considered as a novel biomarker for lymphoma characterization and for predicting patient survival.
|
|
| 2. |
|
|
| 3. |
|
|
| 4. |
|
|
| 5. |
|
|
| 6. |
|
|
| 7. |
|
|
| 8. |
|
|
| 9. |
|
|
| 10. |
- Huotilainen, C., et al.
(author)
-
Electrochemical investigation of in-service thermal aging in two CF8M cast stainless steels
- 2019
-
In: Journal of Nuclear Materials. - : Elsevier. - 0022-3115 .- 1873-4820. ; 520, s. 34-40
-
Journal article (peer-reviewed)abstract
- In-service thermal aging of CF8M cast austenitic stainless steel was investigated in materials removed from the steam generator inlet and crossover elbows of the Ringhals 2 pressurized water reactor nuclear power plant unit after approximately 92kh of full operating time. The thermal aging of these materials was investigated using the double loop electrochemical potentiokinetic reactivation method, coupled with indentation hardness measurements and microstructural characterizations, to identify correlations between the electrochemical behavior and traditional methods of investigating thermal aging embrittlement effects in cast stainless steels. While this electrochemical method can be easily employed to quantify thermal aging effects in materials aged at higher temperatures (e.g. greater than 350 degrees C), this study highlights the difficulties encountered when electrochemically evaluating the aging of materials exposed to nuclear power plant operating conditions.
|
|
