| 1. |
- Hupało, Kamil, et al.
(författare)
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An urban Blitz with a twist : rapid biodiversity assessment using aquatic environmental DNA
- 2021
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Ingår i: Environmental DNA. - : John Wiley & Sons. - 2637-4943 .- 2637-4943. ; 3:1, s. 200-213
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Tidskriftsartikel (refereegranskat)abstract
- As global biodiversity declines, there is an increasing need to create an educated and engaged society. Having people of all ages participate in measuring biodiversity where they live helps to create awareness. Recently, the use of environmental DNA (eDNA) for biodiversity surveys has gained momentum. Here, we explore whether sampling eDNA and sequencing it can be used as a means of rapidly surveying urban biodiversity for educational purposes. We sampled 2 × 1 L of water from each of 15 locations in the city of Trondheim, Norway, including a variety of freshwater, marine, and brackish habitats. DNA was extracted, amplified in triplicate targeting the barcoding fragment of COI gene, and sequenced. The obtained data were analyzed on the novel mBRAVE platform, an online open‐access software and computing resource. The water samples were collected in 2 days by two people, and the laboratory analysis was completed in 5 days by one person. Overall, we detected the presence of 506 BINs identified as belonging to 435 taxa, representing at least 265 putative species. On average, only 5.4% of the taxa were shared among six replicates per site. Based on the observed diversity, three distinct clusters were detected and related to the geographic distribution of sites. There were some taxa shared between the habitats, with a substantial presence of terrestrial biota. Here we propose a new form of BioBlitz, where with noninvasive sampling effort combined with swift processing and straightforward online analyses, hundreds of species can be detected. Thus, using eDNA analysis of water is useful for rapid biodiversity surveys and valuable for educational purposes. We show that rapid eDNA surveys, combined with openly available services and software, can be used as an educational tool to raise awareness about the importance of biodiversity.
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| 2. |
- Ivanov, Ivan, et al.
(författare)
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Advanced Data Processing for Monitoring the Explosiveness of Combustible Gas Mixtures
- 2021
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Ingår i: 2021 10th Mediterranean Conference on Embedded Computing (MECO). - 9781665439121 - 9781665439114 - 9781665429894
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Konferensbidrag (refereegranskat)abstract
- In this paper, a methodology for assessing the explosiveness of hydrocarbon gases combined with hydrogen is proposed. The methodology is based on the measurement of heat released during gas mixture burning in the catalytic sensor. A multi-stage heating profile with different duration and voltage amplitude was developed to divide the catalytic sensor response for hydrogen and hydrocarbons in the mixture. The advanced data processing of experimental data was used to calculate the heat released. The methodology does not require the identification of the gas, their quantity and concentration.
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| 3. |
- Polishchuk, Dmytr, et al.
(författare)
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Oscillatory exchange bias controlled by RKKY in magnetic multilayers
- 2023
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Ingår i: Applied Physics Letters. - : AIP Publishing. - 0003-6951 .- 1077-3118. ; 122:6, s. 062405-
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Tidskriftsartikel (refereegranskat)abstract
- Ferromagnetic/antiferromagnetic bilayers are interfaced with normal metal/ferromagnetic bilayers to form F*/AF/N/F valves. The N-spacer thickness is chosen such that it mediates strong indirect exchange [Ruderman-Kittel-Kasuya-Yosida (RKKY)] between the outer ferromagnetic layers, which varies in strength/direction depending on the N thickness and changes its direction on switching F. The system exhibits a strong modulation of the F*/AF exchange bias, oscillating in strength synchronously with the oscillation in the interlayer RKKY exchange across the normal metal spacer. The effect is explained as due to a superposition taking place within the antiferromagnetic layer of the direct-exchange proximity effect from the F*/AF interface and the indirect RKKY exchange from F penetrating AF via N. The modulation, expressed via the strength of the F*/AF bias field, reaches 400% at the first RKKY peak.
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| 4. |
- Roslin, Tomas, et al.
(författare)
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A molecular-based identification resource for the arthropods of Finland
- 2022
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Ingår i: Molecular Ecology Resources. - : Wiley. - 1755-098X .- 1755-0998. ; 22:2, s. 803-822
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Tidskriftsartikel (refereegranskat)abstract
- To associate specimens identified by molecular characters to other biological knowledge, we need reference sequences annotated by Linnaean taxonomy. In this study, we (1) report the creation of a comprehensive reference library of DNA barcodes for the arthropods of an entire country (Finland), (2) publish this library, and (3) deliver a new identification tool for insects and spiders, as based on this resource. The reference library contains mtDNA COI barcodes for 11,275 (43%) of 26,437 arthropod species known from Finland, including 10,811 (45%) of 23,956 insect species. To quantify the improvement in identification accuracy enabled by the current reference library, we ran 1000 Finnish insect and spider species through the Barcode of Life Data system (BOLD) identification engine. Of these, 91% were correctly assigned to a unique species when compared to the new reference library alone, 85% were correctly identified when compared to BOLD with the new material included, and 75% with the new material excluded. To capitalize on this resource, we used the new reference material to train a probabilistic taxonomic assignment tool, FinPROTAX, scoring high success. For the full-length barcode region, the accuracy of taxonomic assignments at the level of classes, orders, families, subfamilies, tribes, genera, and species reached 99.9%, 99.9%, 99.8%, 99.7%, 99.4%, 96.8%, and 88.5%, respectively. The FinBOL arthropod reference library and FinPROTAX are available through the Finnish Biodiversity Information Facility (www.laji.fi) at https://laji.fi/en/theme/protax. Overall, the FinBOL investment represents a massive capacity-transfer from the taxonomic community of Finland to all sectors of society.
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