| 1. |
|
|
| 2. |
|
|
| 3. |
- Beziat, Vivien, et al.
(författare)
-
NK cell responses to cytomegalovirus infection lead to stable imprints in the human KIR repertoire and involve activating KIRs
- 2013
-
Ingår i: Blood. - : American Society of Hematology. - 0006-4971 .- 1528-0020. ; 121:14, s. 2678-2688
-
Tidskriftsartikel (refereegranskat)abstract
- Human natural killer (NK) cells are functionally regulated by killer cell immunoglobulin-like receptors (KIRs) and their interactions with HLA class I molecules. As KIR expression in a given NK cell is genetically hard-wired, we hypothesized that KIR repertoire perturbations reflect expansions of unique NK-cell subsets and may be used to trace adaptation of the NK-cell compartment to virus infections. By determining the human KIR-ome at a single-cell level in more than 200 donors, we were able to analyze the magnitude of NK cell adaptation to virus infections in healthy individuals. Strikingly, infection with human cytomegalovirus (CMV), but not with other common herpesviruses, induced expansion and differentiation of KIR-expressing NK cells, visible as stable imprints in the repertoire. Education by inhibitory KIRs promoted the clonal-like expansion of NK cells, causing a bias for self-specific inhibitory KIRs. Furthermore, our data revealed a unique contribution of activating KIRs (KIR2DS4, KIR2DS2, or KIR3DS1), in addition to NKG2C, in the expansion of human NK cells. These results provide new insight into the diversity of KIR repertoire and its adaptation to virus infection, suggesting a role for both activating and inhibitory KIRs in immunity to CMV infection.
|
|
| 4. |
- Edvardsson, Arne, et al.
(författare)
-
Is a cognitive-behavioural biofeedback intervention useful to reduce injury risk in junior football players?
- 2012
-
Ingår i: Journal of Sports Science and Medicine (JSSM). - Bursa, Turkey : Journal of Sports Science and Medicine. - 1303-2968. ; 11:2, s. 331-338
-
Tidskriftsartikel (refereegranskat)abstract
- Athletes participating in sport are exposed to a relatively high injury risk. Previous research has suggested that it could be possible to reduce sports injuries through psychological skills training. The purpose of this study was to examine the extent to which a cognitive behavioural biofeedback intervention could reduce the number of sports injuries in a sample of players in Swedish elite football high schools. Participants from four elite football high schools (16-19 years old) were divided into one experiment (n = 13) and one control group (n = 14). Participants were asked to complete three questionnaires to assess anxiety level (Sport Anxiety Scale), history of stressors (Life Event Scale for Collegiate Athletes) and coping skills (Athletic Coping Skills Inventory - 28) in a baseline measure. Mann-Whitney Utests showed no significant differences in pre-intervention scores based on the questionnaires. The experimental group participated in a nine-week intervention period consisting of seven sessions, including: somatic relaxation, thought stopping, emotions/problem focused coping, goal setting, biofeedback training as well as keeping a critical incident diary. A Mann-Whitney U test showed no significant difference between the control and experimental group U (n1 = 13, n2 = 14) = 51.00, p = 0.054. However, considering the small sample, the statistical power (0.05 for present study), to detect effects was low. The results of the study are discussed from a psychological perspective and proposals for future research are given. © Journal of Sports Science and Medicine.
|
|
| 5. |
- Ernst, Andreas, et al.
(författare)
-
A Structural Portrait of the PDZ Domain Family
- 2014
-
Ingår i: Journal of Molecular Biology. - : Elsevier BV. - 0022-2836 .- 1089-8638. ; 426:21, s. 3509-3519
-
Tidskriftsartikel (refereegranskat)abstract
- PDZ (PSD-95/Discs-large/ZO1) domains are interaction modules that typically bind to specific C-terminal sequences of partner proteins and assemble signaling complexes in multicellular organisms. We have analyzed the existing database of PDZ domain structures in the context of a specificity tree based on binding specificities defined by peptide-phage binding selections. We have identified 16 structures of PDZ domains in complex with high-affinity ligands and have elucidated four additional structures to assemble a structural database that covers most of the branches of the PDZ specificity tree. A detailed comparison of the structures reveals features that are responsible for the diverse specificities across the PDZ domain family. Specificity differences can be explained by differences in PDZ residues that are in contact with the peptide ligands, but these contacts involve both side-chain and main-chain interactions. Most PDZ domains bind peptides in a canonical conformation in which the ligand main chain adopts an extended β-strand conformation by interacting in an antiparallel fashion with a PDZ β-strand. However, a subset of PDZ domains bind peptides with a bent main-chain conformation and the specificities of these non-canonical domains could not be explained based on canonical structures. Our analysis provides a structural portrait of the PDZ domain family, which serves as a guide in understanding the structural basis for the diverse specificities across the family.
|
|
| 6. |
- Fallby, Johan, 1968-, et al.
(författare)
-
Parental support as a predictors to success in adolescent male football
- 2011
-
Ingår i: Proceedings of the 13th European Congress of Sport Psychology, Madeira, Portugal. FEPSAC on-line publication. ; , s. 308-
-
Konferensbidrag (refereegranskat)abstract
- The aim of the study was to examine if parental support could predict elite academy participation in an adolescent football population. The participants were 767 adolescent male football players, where 443 represented an academy team and 324 represented lower ranked teams, in age between 11 and 18. The participants were classified into three groups; a) children (11-12 years), b) youth (13-15 years) and c) junior (16-18 years). The questionnaire used was the Swedish health survey developed by the Swedish Health Institute with a number of football specific items added. Parental support was measured with six items that all measured emotional support (for example if the player experience that his parents understand, listen to, and treat him fair).One way ANOVA showed that academy players reported significant higher level of parental support then the non–academy players in children (F(1, 196)= 7,071, p = 0,008) and junior ages (F(1,194) = 10,830, p = 0,001). A logistic regression showed that parental support predicted approximately 68% of the players belonging accurate both in the children- (68,2%) and junior (67,9%) sample.The result supports previous findings showing that adaptive coping resources, such as social support seeking, could predict athletic success (Yperen, 2009). One recommendation for football clubs with youth academies is to involve parents in the social support network in order to give the players more adaptive coping resources. Further, educating parents about demands and career transitions that the players are exposed to in an elite academy could be beneficial in a developmental perspective.
|
|
| 7. |
|
|
| 8. |
|
|
| 9. |
|
|
| 10. |
- Hämälistö, Saara, et al.
(författare)
-
A ZO-1/α5β1-integrin complex regulates cytokinesis downstream of PKCε in NCI-H460 cells plated on fibronectin
- 2013
-
Ingår i: PLOS ONE. - : Public Library of Science (PLoS). - 1932-6203. ; 8:8, s. e70696-
-
Tidskriftsartikel (refereegranskat)abstract
- Recently, we demonstrated that integrin adhesion to the extracellular matrix at the cleavage furrow is essential for cytokinesis of adherent cells. Here, we report that tight junction protein ZO-1 (Zonula Occludens-1) is required for successful cytokinesis in NCI-H460 cells plated on fibronectin. This function of ZO-1 involves interaction with the cytoplasmic domain of α5-integrin to facilitate recruitment of active fibronectin-binding integrins to the base of the cleavage furrow. In the absence of ZO-1, or a functional ZO-1/α5β1-integrin complex, proper actin-dependent constriction between daughter cells is impaired and cells fail cytokinesis. Super-resolution microscopy reveals that in ZO-1 depleted cells the furrow becomes delocalized from the matrix. We also show that PKCε-dependent phosphorylation at Serine168 is required for ZO-1 localization to the furrow and successful cell division. Altogether, our results identify a novel regulatory pathway involving the interplay between ZO-1, α5-integrin and PKCε in the late stages of mammalian cell division.
|
|
