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Sökning: WFRF:(Iyengar Sharath) > (2022) > Bacteria Detection ...

Bacteria Detection at a Single-Cell Level through a Cyanotype-Based Photochemical Reaction

Dietvorst, Jiri (författare)
CSIC, Inst Microelect Barcelona IMB CNM, Bellaterra 08193, Barcelona, Spain.;CSIC, Inst Adv Chem Catalonia IQAC, Dept Chem & Biomol Nanotechnol, Nanobiotechnol Diagnost Nb4D, Barcelona 08034, Spain.
Ferrer-Vilanova, Amparo (författare)
CSIC, Inst Microelect Barcelona IMB CNM, Bellaterra 08193, Barcelona, Spain.;Univ Autonoma Barcelona, Dept Quim, Bellaterra 08193, Barcelona, Spain.
Iyengar, Sharath Narayana (författare)
KTH,Nanobioteknologi,Science for Life Laboratory, SciLifeLab
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Russom, Aman, Prof. 1976- (författare)
KTH,Nanobioteknologi,Science for Life Laboratory, SciLifeLab
Vigues, Nuria (författare)
Univ Autonoma Barcelona, Dept Genet & Microbiol, Bellaterra 08193, Barcelona, Spain.
Mas, Jordi (författare)
Univ Autonoma Barcelona, Dept Genet & Microbiol, Bellaterra 08193, Barcelona, Spain.
Vilaplana, Lluisa (författare)
CSIC, Inst Adv Chem Catalonia IQAC, Dept Chem & Biomol Nanotechnol, Nanobiotechnol Diagnost Nb4D, Barcelona 08034, Spain.
Marco, Maria-Pilar (författare)
CSIC, Inst Adv Chem Catalonia IQAC, Dept Chem & Biomol Nanotechnol, Nanobiotechnol Diagnost Nb4D, Barcelona 08034, Spain.;CIBER Bioingn Biomat & Nanomed CIBER BBN, Barcelona 08034, Spain.
Guirado, Gonzalo (författare)
Univ Autonoma Barcelona, Dept Quim, Bellaterra 08193, Barcelona, Spain.
Munoz-Berbel, Xavier (författare)
CSIC, Inst Microelect Barcelona IMB CNM, Bellaterra 08193, Barcelona, Spain.
visa färre...
CSIC, Inst Microelect Barcelona IMB CNM, Bellaterra 08193, Barcelona, Spain;CSIC, Inst Adv Chem Catalonia IQAC, Dept Chem & Biomol Nanotechnol, Nanobiotechnol Diagnost Nb4D, Barcelona 08034, Spain. CSIC, Inst Microelect Barcelona IMB CNM, Bellaterra 08193, Barcelona, Spain.;Univ Autonoma Barcelona, Dept Quim, Bellaterra 08193, Barcelona, Spain. (creator_code:org_t)
2021-12-21
2022
Engelska.
Ingår i: Analytical Chemistry. - : American Chemical Society (ACS). - 0003-2700 .- 1520-6882. ; 94:2, s. 787-792
  • Tidskriftsartikel (refereegranskat)
Abstract Ämnesord
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  • The detection of living organisms at very low concentrations is necessary for the early diagnosis of bacterial infections, but it is still challenging as there is a need for signal amplification. Cell culture, nucleic acid amplification, or nano-structure-based signal enhancement are the most common amplification methods, relying on long, tedious, complex, or expensive procedures. Here, we present a cyanotype-based photochemical amplification reaction enabling the detection of low bacterial concentrations up to a single-cell level. Photocatalysis is induced with visible light and requires bacterial metabolism of iron-based compounds to produce Prussian Blue. Bacterial activity is thus detected through the formation of an observable blue precipitate within 3 h of the reaction, which corresponds to the concentration of living organisms. The short time-to-result and simplicity of the reaction are expected to strongly impact the clinical diagnosis of infectious diseases.

Ämnesord

NATURVETENSKAP  -- Kemi -- Analytisk kemi (hsv//swe)
NATURAL SCIENCES  -- Chemical Sciences -- Analytical Chemistry (hsv//eng)

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