| 1. |
- Jönsson, Charlotta, et al.
(författare)
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The effects of pole walking on arm lymphedema and cardiovascular fitness in women treated for breast cancer: a pilot and feasibility study.
- 2014
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Ingår i: Physiotherapy Theory and Practice. - : Informa UK Limited. - 0959-3985 .- 1532-5040. ; 30:4, s. 236-242
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Tidskriftsartikel (refereegranskat)abstract
- Abstract The benefit of exercise for breast cancer-treated women is well documented. However, studies of cardiovascular fitness training for women with breast cancer-related arm lymphedema are rare. The purpose of this study was to investigate the effects of intensive pole walking on arm lymphedema in women treated for breast cancer. Thirty-five women with unilateral lymphedema were included and twenty-three completed an eight-week exercise intervention consisting of pole walking 3-5 times per week, for 30-60 min, at 70%-80% of their maximum heart rate, preceded by a two-week control period. Measurements of arm lymphedema (water displacement method), body weight, cardiovascular fitness (sub-maximal bicycle ergometer test) and subjective assessments (disability of the arm, shoulder and hand (DASH) questionnaire; heaviness and tightness using a visual analogue scale (VAS); and well-being) were performed before the control period and before and after the exercise intervention. The results indicated a significant reduction in total arm volume of the lymphedema arm (p = 0.001), in lymphedema absolute volume (p = 0.014) and lymphedema relative volume (p = 0.015). Significant decreases of heart rate (p = 0.004), DASH score (p = 0.053) and rating of tightness in the arm (p = 0.043) were found. Positive and negative influences on well-being were reported. The conclusion of this study is that pole walking is feasible for breast cancer-treated women with arm lymphedema.
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| 2. |
- Jönsson, Håkan, et al.
(författare)
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När mjölken blev skånsk
- 2010
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Ingår i: Skåne, mat och medier. ; , s. 39-54
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Bokkapitel (populärvet., debatt m.m.)
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| 3. |
- Nilsson, Charlotta, et al.
(författare)
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Element analysis of the eutardigrades Richtersius coronifer and Milnesium cf. asiaticum using Particle Induced X-ray Emission (PIXE)
- 2013
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Ingår i: Journal of limnology. - : Page Press. - 1129-5767 .- 1723-8633. ; 72:s1, s. 92-101
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Tidskriftsartikel (refereegranskat)abstract
- Semi-terrestrial tardigrades are well-known for their tolerance to a variety of environmental extremes, including desiccation, freezing and radiation. Despite several attempts to reveal the genetic and molecular mechanisms behind the resilience of tardigrades, it is still unknown how these animals are able to maintain the integrity of their cellular components under severe stress. Quantitative or qualitative changes in molecular compounds (e.g., carbohydrates, proteins) are expected, and have been the main line of research towards understanding the tolerance of tardigrades. In radiation tolerant bacteria, a tolerance mechanism based on manganese has been proposed. We evaluate this hypothesis in tardigrades and provide the first data on element composition in desiccated and non-desiccated specimens of two eutardigrade species, Richtersius coronifer and Milnesium cf. asiaticum. A focused 2 MeV proton microbeam was utilised to determine the elemental content, distributions and concentrations, using the ion beam analytical technique particle induced X-ray emission(PIXE). The presence of six elements – phosphorus, sulphur, chlorine, potassium, calcium and iron – were confirmed in all tardigrade specimens, at levels up to a few mg g–1. However, manganese was found in less than 10% of the analysed specimens, and in low amounts, thus our study provides no evidence for the manganese hypothesis. We also show that the distributions and/or concentrations of some elements differ between the two species as well as between the dehydrated and hydrated state. In particular, very low levels of iron were found in dehydrated M. cf. asiaticum. Our analysis shows that the PIXE technique is a useful tool for investigating questions on the distribution of elements both in dehydrated and hydrated tardigrades.
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| 4. |
- Nilsson, Charlotta, et al.
(författare)
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Element analysis of the eutardigrades Richtersius coronifer and Milnesium cf. asiaticum using particle induced X-ray emission (PIXE)
- 2013
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Ingår i: Journal of limnology. - : PAGEPress Publications. - 1129-5767 .- 1723-8633. ; 72, s. 92-101
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Tidskriftsartikel (refereegranskat)abstract
- Semi-terrestrial tardigrades are well-known for their tolerance to a variety of environmental extremes, including desiccation, freezing and radiation. Despite several attempts to reveal the genetic and molecular mechanisms behind the resilience of tardigrades, it is still unknown how these animals are able to maintain the integrity of their cellular components under severe stress. Quantitative or qualitative changes in molecular compounds (e.g., carbohydrates, proteins) are expected, and have been the main line of research towards understanding the tolerance of tardigrades. In radiation tolerant bacteria, a tolerance mechanism based on manganese has been proposed. We evaluate this hypothesis in tardigrades and provide the first data on element composition in desiccated and non-desiccated specimens of two eutardigrade species, Richtersius coronifer and Milnesium cf. asiaticum. A focused 2 MeV proton microbeam was utilised to determine the elemental content, distributions and concentrations, using the ion beam analytical technique particle induced X-ray emission (PIXE). The presence of six elements - phosphorus, sulphur, chlorine, potassium, calcium and iron - were confirmed in all tardigrade specimens, at levels up to a few mg g(-1). However, manganese was found in less than 10% of the analysed specimens, and in low amounts, thus our study provides no evidence for the manganese hypothesis. We also show that the distributions and/or concentrations of some elements differ between the two species as well as between the dehydrated and hydrated state. In particular, very low levels of iron were found in dehydrated M. cf. asiaticum. Our analysis shows that the PIXE technique is a useful tool for investigating questions on the distribution of elements both in dehydrated and hydrated tardigrades.
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| 5. |
- Nilsson, Charlotta, et al.
(författare)
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Tolerance to proton irradiation in the eutardigrade Richtersius coronifer - a nuclear microprobe study
- 2010
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Ingår i: International Journal of Radiation Biology. - : Informa UK Limited. - 0955-3002 .- 1362-3095. ; 86:5, s. 420-427
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Tidskriftsartikel (refereegranskat)abstract
- Materials and methods: Dehydrated tardigrades of the species R. coronifer were irradiated with 2.55 MeV (megaelectronvolts) protons at doses ranging from 500 gray (Gy) to 15,000 Gy, to investigate the dose-viability relationship. In addition, a focused proton microbeam was utilised to determine the areal mass distribution, using the ion beam analytical technique STIM (Scanning Transmission Ion Microscopy). Results: The experiment suggests that R. coronifer is unaffected by doses of proton irradiation up to 10,000 Gy, but shows very little viability at higher doses. The STIM analysis revealed that the thickness of the dehydrated tardigrades exceeds 150 mu m, and that a fraction of the protons may not be fully absorbed. Conclusion: Our results are in line with previous studies of exposure to high-LET radiation in tardigrades, indicating that these animals are equally or even more tolerant to high-LET compared to low-LET gamma radiation. The physiological background to this remarkable result is currently unknown, but deserves investigation.
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| 6. |
- Nilsson, E J Charlotta, et al.
(författare)
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Evaluation of cryoanalysis as a tool for analyzing elemental distributionin ‘‘live’’ tardigrades using micro-PIXE
- 2014
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Ingår i: Nuclear Instruments and Methods in Physics Research Section B. - 0168-583X .- 1872-9584. ; 332, s. 181-186
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Tidskriftsartikel (refereegranskat)abstract
- Although heavy on labor and equipment, thus not often applied, cryoanalysis of frozen hydrated biological specimens can provide information that better reflects the living state of the organism, compared with analysis in the freeze-dried state. In this paper we report a study where the cryoanalysis facility with cryosectioning capabilities at Materials Research Department, iThemba LABS, South Africa was employed to evaluate the usefulness of combining three ion beam analytical methods (μPIXE, RBS and STIM) to analyze a biological target where a better elemental compositional description is needed – the tardigrade. Imaging as well as quantification results are of interest.In a previous study, the element composition and redistribution of elements in the desiccated and active states of two tardigrade species was investigated. This study included analysis of both whole and sectioned tardigrades, and the aim was to analyze each specimen twice; first frozen hydrated and later freeze-dried. The combination of the three analytical techniques proved useful: elements from C to Rb in the tardigrades could be determined and certain differences in distribution of elements between the frozen hydrated and the freeze-dried states were observed. RBS on frozen hydrated specimens provided knowledge of matrix elements.
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| 7. |
- Nilsson, E. J. Charlotta, et al.
(författare)
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Tolerance to proton irradiation in the eutardigrade Richtersius coronifer : a nuclear microprobe study
- 2010
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Ingår i: International Journal of Radiation Biology. - 0955-3002 .- 1362-3095. ; 86:5, s. 420-427
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Tidskriftsartikel (refereegranskat)abstract
- Purpose:The tardigrade Richtersius coronifer has previously been shown to tolerate very high doses of low linear energy transfer (low-LET) radiation (gamma rays). The purpose of this study was to extend our knowledge on radiation tolerance in this species by investigating the dose-response to high-LET radiation in terms of protons.Materials and methods:Dehydrated tardigrades of the species R. coronifer were irradiated with 2.55 MeV (megaelectronvolts) protons at doses ranging from 500 gray (Gy) to 15,000 Gy, to investigate the dose-viability relationship. In addition, a focused proton microbeam was utilised to determine the areal mass distribution, using the ion beam analytical technique STIM (Scanning Transmission Ion Microscopy).Results:The experiment suggests that R. coronifer is unaffected by doses of proton irradiation up to 10,000 Gy, but shows very little viability at higher doses. The STIM analysis revealed that the thickness of the dehydrated tardigrades exceeds 150 μm, and that a fraction of the protons may not be fully absorbed.Conclusion:Our results are in line with previous studies of exposure to high-LET radiation in tardigrades, indicating that these animals are equally or even more tolerant to high-LET compared to low-LET gamma radiation. The physiological background to this remarkable result is currently unknown, but deserves investigation.
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| 8. |
- Rasmusson, Elisabeth, et al.
(författare)
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Low rate of lymphedema after extended pelvic lymphadenectomy followed by pelvic irradiation of node-positive prostate cancer
- 2013
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Ingår i: Radiation Oncology. - 1748-717X. ; 8
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Tidskriftsartikel (refereegranskat)abstract
- Background: The aim of the present study was to evaluate the prevalence and severity of lower limb lymphedema after pelvic lymphadenectomy and radiotherapy to the pelvic lymph nodes in patients with prostate cancer. Methods: Twenty-six patients underwent combined treatment for high-risk node-positive prostate cancer at Skane University Hospital between April 2008 and March 2011. The treatment consisted of extended pelvic lymphadenectomy followed by androgen deprivation therapy and radiotherapy. The pelvic lymphnodes, prostate and seminal vesicles were treated with external beam radiotherapy (EBRT) to an absorbed dose of 50 Gy followed by a brachytherapy (BT) boost of 2x10 Gy to the prostate only. Twenty-two patients accepted an invitation to a clinical examination with focus on lower limb swelling. The median time between the end of radiotherapy and examination was 2.2 years (range 1.2-4.1). Results: Six patients (27%) experienced grade 1 lymphedema and two patients (9%) grade 2 while none had grade 3 or 4 according to the CTC Common Toxicity Criteria scale 4.0. Three patients required treatment with compression stockings. Conclusion: Brachytherapy and pelvic EBRT have a low incidence of lymphedema (at median 2.2 y after treatment) in patients with high-risk node-positive prostate cancer that have undergone pelvic lymph node dissection.
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| 9. |
- Saldert, Charlotta, 1966, et al.
(författare)
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Combining methods in the assessment and analysis of communication in aphasia: Benefits and shortcomings of different approaches
- 2012
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Ingår i: Journal of Interactional Research in Communication Disorders. - : Equinox Publishing. - 2040-512X .- 2040-5111. ; 3:2, s. 141-169
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Tidskriftsartikel (refereegranskat)abstract
- In this case study the benefit of using analysis of conversational interaction in clinical assessment of communication is explored. A diagnostic test revealed a severe global aphasia in a man. However, according to a questionnaire filled out by the wife, her husband had some functional communication. The description of the consequences of aphasia in this man was further modified and enriched by analyses of conversational interaction between the man and his spouse. The Measure of Participation in Conversation and the Measure of Skill in Supported Conversation show that the couple has strategies that facilitate communication. Activity-based Communication Analysis provides information about how the strategies are used and about the influence of different factors brought into the interaction by the situation and the individuals involved. Thus, although time consuming, analysis of natural conversations provide valid information that is of importance in assessment of communication and implementation of intervention that can have an impact on everyday life in persons with aphasia and their conversation partners.
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| 10. |
- Tuominen, Rainer, et al.
(författare)
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Investigation of a putative melanoma susceptibility locus at chromosome 3q29
- 2014
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Ingår i: Cancer Genetics. - : Elsevier. - 2210-7762 .- 2210-7770. ; 207:3, s. 70-74
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Tidskriftsartikel (refereegranskat)abstract
- Malignant melanoma, the most fatal form of skin cancer, is currently increasing in incidence in many populations. Approximately 10% of all cases occur in families with an inherited predisposition for melanoma. In Sweden, only a minor portion of such melanoma families carry a mutation in the known melanoma gene CDKN2A, and there is a need to identify additional melanoma susceptibility genes. In a recently performed genome-wide linkage screen, novel loci with suggestive evidence of linkage to melanoma were detected. In this study, we have further analyzed one region on chromosome 3q29. In all, 89 affected and 15 nonaffected family members from 42 melanoma-prone families were genotyped for 34 genetic markers. In a pooled linkage analysis of all 42 families, we detected significant evidence of linkage, with a maximum heterogeneity logarithm of odds (HLOD) score of 3.1 with 83% of the families contributing to the linkage score. The minimum critical region of linkage (defined by a 1LOD score support interval) maps to chromosome 3q29, spans 3.5 Mb of genomic sequence, and harbors 44 identified genes. Sequence variants within this region have previously been associated with cancer susceptibility. This study reports the presence of a putative novel melanoma susceptibility locus in the Swedish population, a finding that needs to be replicated in an independent study on other individuals with familial melanoma. Sequencing of genes in the region may identify novel melanoma-associated mutations.
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