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- Jansson, Nina, 1976, et al.
(författare)
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Leptin stimulates the activity of the system A amino acid transporter in human placental villous fragments.
- 2003
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Ingår i: The Journal of clinical endocrinology and metabolism. - 0021-972X. ; 88:3, s. 1205-11
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Tidskriftsartikel (refereegranskat)abstract
- The activity and expression of placental nutrient transporters are primary determinants for the supply of nutrients to the fetus, and these nutrients in turn regulate fetal growth. We developed an experimental system to assess amino acid uptake in single primary villous fragments to study hormonal regulation of the amino acid transporter system A in term human placenta. Validation of the method, using electron microscopy and studies of hormone production, indicated that fragments maintained ultrastructural and functional integrity for at least 3 h. The activity of system A was measured as the Na(+)-dependent uptake of methylaminoisobutyric acid (MeAIB), and the effect of 1 h incubation in various hormones was investigated. Uptake of MeAIB into villous fragments in the presence of Na(+) was linear up to at least 30 min. Insulin (300 ng/ml, n = 14) increased system A activity by 56% (P < 0.05). This effect was also present at insulin concentrations in the physiological range (+47% at 0.6 ng/ml, n = 10, P < 0.05). Leptin (500 ng/ml, n = 14) increased Na(+)-dependent MeAIB uptake by 37% (P < 0.05). System A activity increased in a concentration-dependent fashion in response to leptin (n = 10). However, neither epidermal GF (600 ng/ml), cortisol (340 ng/ml), nor GH (500 ng/ml) altered system A activity significantly (n = 14). We conclude that primary single isolated villous fragments can be used in studies of hormonal regulation of nutrient uptake into the syncytiotrophoblast. These data suggest that leptin regulates system A, a key amino acid transporter.
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- Jansson, S. E. A., et al.
(författare)
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Packaging materials for fermented milk : Effects of material crystallinity and polarity on food quality
- 2001
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Ingår i: Packaging technology & science. - : Wiley. - 0894-3214 .- 1099-1522. ; 14:3, s. 119-127
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Tidskriftsartikel (refereegranskat)abstract
- The ability of a packaging material to protect the food product and extend its shelf-life depends on several material properties. In this work the effects of material crystallinity and polarity on the quality of fermented milk were studied. The fermented milk is a high-quality Swedish product, similar to yoghurt. The quality of the food product was determined as a function of storage time by containing the liquid in pouches of different materials. The material crystallinity was varied by using very low-density polyethylene, high-density polyethylene and aluminium laminate as packaging materials. Aluminium was used on account of its '100%' gas-tightness. The polarity was varied by comparing an aliphatic polyketone with polyethylene of similar crystallinity. The carbon dioxide (CO2) and oxygen (O-2) contents in the headspace of the pouches were determined. The food quality was determined by measuring whey syneresis, viscosity and the content of desired Bifidobacteria, as well as of undesired yeast and mould. A trained taste panel determined the degree of acidity and of the sparkling taste. It was found that the content of CO2 increased and that of O-2 decreased in the pouches with increasing degree of crystallinity and increasing polarity. The sparkling taste of fermented milk was a clear function of the headspace CO2 content. The data presented here could thus be used to 'design' a package for a desired sparkling taste of the fermented milk by selecting a certain material crystallinity. Whey syneresis, viscosity and content of Bifidobacteria were found to be independent of pouch material. While the degree of whey syneresis and the viscosity increased with increasing storage time, the content of Bifidobacteria slowly decreased. The content of yeast and mould in the liquid was below the existing limit values for foodstuffs. The degrees of acidity and sparkling taste were highest for the liquids contained in aluminium and polyketone pouches, although the differences in acidulous taste between the various pouch materials were small.
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- Jansson, S. E. A., et al.
(författare)
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Packaging materials for fermented milk Part 2 : Solute-induced changes and effects of material polarity and thickness on food quality
- 2002
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Ingår i: Packaging technology & science. - : Wiley. - 0894-3214 .- 1099-1522. ; 15:6, s. 287-300
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Tidskriftsartikel (refereegranskat)abstract
- This work is a continuation of the application of a developed methodology for the selection of packaging material for a specific food product, in this case the 'demanding' food product. fermented milk. The effects of different packaging material parameters on the quality of fermented milk were studied. Food quality after storage was determined as a function of material polarity and pouch thickness by storing the liquid in pouches of different materials. The material polarity was varied by using laminates with polyethylene, poly(ethylene-co-vinyl alcohol) with two different ethylene contents and an aliphatic polyketone. The effects of pouch thickness were studied using high-density polyethylene films of different thicknesses between 25 mum and 200 mum. The interactions between the milk product and the pouch material were analysed by oxygen and water permeability, gas chromatography-mass spectrometry and tensile testing. The CO2 and O-2 contents in the headspace of the pouches were determined. The food quality was determined by measuring whey syneresis and the contents of Bifidobacteria, Enterobacteriaceae, yeast and mould. A trained taste panel determined the sensory properties. The content of CO2, and consequently the sparkling taste, increased with increasing polarity and/or pouch thickness. The CO2 content was affected more easily by changes in material polarity than by changes in pouch thickness. The increase in whey syneresis and the decrease in Bifidobacteria content with time were independent Of material polarity and pouch thickness. The contents Of Enterobacteriaceae, yeast and mould in the liquid were always below existing limits for foodstuffs. A newly developed method was used by which the CO2 and 02 permeabilities of the pouch/packaging could be estimated, using the kinetics of the gas composition in the pouch headspace. Permeability values, as estimated by the method, revealed that the CO2 production and the O-2 consumption rates of the fermented milk were dependent on the CO2 and O-2 headspace concentrations. An increase in permeability, determined by conventional methods, suggested that both the non-polar and the polar polymers were plasticized by fermented milk. The plasticization was, however, modest and undetectable when the polyethylene tensile test data were analysed.
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- Lescot, M, et al.
(författare)
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Annotation of a 95-kb Populus deltoides genomic sequence reveals a disease resistance gene cluster and novel class I and class II transposable elements.
- 2004
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Ingår i: Theoretical and Applied Genetics. - : Springer Science and Business Media LLC. - 0040-5752 .- 1432-2242. ; 109:1, s. 10-22
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Tidskriftsartikel (refereegranskat)abstract
- Poplar has become a model system for functional genomics in woody plants. Here, we report the sequencing and annotation of the first large contiguous stretch of genomic sequence (95 kb) of poplar, corresponding to a bacterial artificial chromosome clone mapped 0.6 centiMorgan from the Melampsora larici-populina resistance locus. The annotation revealed 15 putative genetic objects, of which five were classified as hypothetical genes that were similar only with expressed sequence tags from poplar. Ten putative objects showed similarity with known genes, of which one was similar to a kinase. Three other objects corresponded to the toll/interleukin-1 receptor/nucleotide-binding site/leucine-rich repeat class of plant disease resistance genes, of which two were predicted to encode an amino terminal nuclear localization signal. Four objects were homologous to the Ty1/copia family of class I transposable elements, one of which was designated Retropop and interrupted one of the disease resistance genes. Two other objects constituted a novel Spm-like class II transposable element, which we designated Magali.
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