| 1. |
- Fredlund, Kenneth M., et al.
(författare)
-
Comparison of the Stereospecificity and Immunoreactivity of NADH-Ferricyanide Reductases in Plant Membranes
- 1994
-
Ingår i: Plant Physiology. - 0032-0889 .- 1532-2548. ; 106:3, s. 1103-1106
-
Tidskriftsartikel (refereegranskat)abstract
- The substrate stereospecificity of NADH-ferricyanide reductase activities in the inner mitochondrial membrane and peroxisomal membrane of potato (Solanum tuberosum L.) tubers, spinach (Spinacea oleracea L.) leaf plasma membrane, and red beetroot (Beta vulgaris L.) tonoplast were all specific for the [beta]-hydrogen of NADH, whereas the reductases in wheat root (Triticum aestivum L.) endoplasmic reticulum and potato tuber outer mitochondrial membrane were both [alpha]-hydrogen specific. In all isolated membrane fractions one or several polypeptides with an apparent size of 45 to 55 kD cross-reacted with antibodies raised against a microsomal NADH-ferricyanide reductase on western blots.
|
|
| 2. |
- Jean-Faucher, C, et al.
(författare)
-
Developmental and hormonal regulation of specific proteins in mouse vas deferens and seminal vesicle.
- 1991
-
Ingår i: International Journal of Developmental Biology. - 0214-6282 .- 1696-3547. ; 35:2, s. 77-82
-
Tidskriftsartikel (refereegranskat)abstract
- This paper is concerned with hormonal regulation of the developmental pattern of major proteins of the mouse vas deferens (mouse vas deferens protein: MVDP, 34.5 kD) and seminal vesicle (15.5, 120 and 140 kD) whose expression is regulated by testosterone at adulthood. The ontogeny of these proteins, studied by SDS-polyacrylamide gel electrophoresis, appeared to be uncoordinated. MVDP was not accumulated until animals were 20 days old and its concentration increased sharply from 20 to 30 days of age. In seminal vesicle, the 15.5 kD protein did not accumulate before day 30 whereas 120 and 140 kD proteins appeared and accumulated between 30 and 40 days. In 30-day-old mice castrated at birth or treated with cyproterone acetate over 29 days, MVDP levels were not abolished and were similar to those measured in 20-day-old males. Testosterone administration, from 1 to 10 days of age, did not induce precocious expression of MVDP. These results suggest that the neonatal expression of MVDP is independent of androgens. In seminal vesicle, the first expression of the 3 proteins studied was dependent upon testicular androgens as shown by neonatal castration and injection experiments. The marked increase in the levels of the 4 proteins studied, during sexual maturation, was not associated with quantitative or qualitative changes in tissular androgen concentrations, suggesting that other factors may be necessary for protein expression. Whereas thyroxine may induce a precocious accumulation of MVDP, prolactin had no stimulatory effect on the accumulation of proteins from vas deferens and seminal vesicle. The results suggest that during sexual maturation gene activation by androgens was progressive.
|
|
| 3. |
- Haby, Christelle, et al.
(författare)
-
Inhibition of serine/threonine protein phosphatases promotes opening of voltage-activated L-type Ca2+ channels in insulin-secreting cells
- 1994
-
Ingår i: Biochemical Journal. - 0264-6021 .- 1470-8728. ; 298:Pt 2, s. 341-346
-
Tidskriftsartikel (refereegranskat)abstract
- The biological activity of many proteins, including voltage-sensitive ion channels, is controlled by their state of phosphorylation. Ca2+ influx through voltage-activated L-type Ca2+ channels serves as the major stimulatory signal in insulin-secreting cells. We have now investigated the extent to which Ca2+ handling in clonal insulin-secreting RiNm5F cells was affected by okadaic acid, an inhibitor of various serine/threonine protein phosphatases. Whole-cell patch-clamp experiments showed that okadaic acid generated an increase in membrane current, suggesting that it promotes Ca2+ influx through L-type voltage-gated Ca2+ channels probably by modifying their phosphorylation state. Okadaic acid was found to provoke a transient rise in the cytoplasmic free Ca2+ concentration ([Ca2+]i) but had no further effect on the K(+)-induced increase. The Ca2+ transient induced by okadaic acid was dependent on the presence of extracellular Ca2+ and was abolished by D600, a blocker of voltage-activated L-type Ca2+ channels. Concomitant with the rise in [Ca2+]i, okadaic acid induced insulin secretion, a phenomenon that was also dependent on extracellular Ca2+. It is proposed that hyperphosphorylation of voltage-activated L-type Ca2+ channels in insulin-secreting cells lowers the threshold potential for their activation.
|
|
| 4. |
- Vought, Lena B. M., et al.
(författare)
-
Nutrient retention in riparian ecotones
- 1994
-
Ingår i: Ambio. - 0044-7447 .- 1654-7209. ; 23:6, s. 342-348
-
Tidskriftsartikel (refereegranskat)abstract
- Nutrient retention mechanisms in riparian buffer strips are reviewed with emphasis on surface runoff and subsurface flows, the main pathways of exchanges between the stream and its surroundings. Unique physical and biogeochemical properties prevailing in these riparian ecotones dictate the flux of water, nutrients and other exogenous substances between the upland areas and the stream. Removal of nutrients from surface inflows is induced by deposition of sediment bound nutrients and exchange of dissolved nutrients with the soil/litter surface. Removal of nitrogen in subsurface flows can partly be explained by vegetation uptake, but the main mechanism for removal is usually denitrification. In channelized streams, the subsurface inflows have, in most cases, been altered to discharges via drainage tiles, with the exchange flows (water leaving and re-entering the open-channel via the stream bed and banks) being greatly decreased. Consequently, to improve nitrogen removal in these systems, these flows have to be intercepted or reestablished either through restoration of the old stream valley or through managed structures in the buffer strips.
|
|
| 5. |
- Vought, Lena B. M., et al.
(författare)
-
Nutrient retention in riparian ecotones
- 1994
-
Ingår i: Ambio. - : Allen Press Inc.. - 0044-7447 .- 1654-7209. ; 23:6, s. 342-348
-
Tidskriftsartikel (refereegranskat)abstract
- Nutrient retention mechanisms in riparian buffer strips are reviewed with emphasis on surface runoff and subsurface flows, the main pathways of exchanges between the stream and its surroundings. Unique physical and biogeochemical properties prevailing in these riparian ecotones dictate the flux of water, nutrients and other exogenous substances between the upland areas and the stream. Removal of nutrients from surface inflows is induced by deposition of sediment bound nutrients and exchange of dissolved nutrients with the soil/litter surface. Removal of nitrogen in subsurface flows can partly be explained by vegetation uptake, but the main mechanism for removal is usually denitrification. In channelized streams, the subsurface inflows have, in most cases, been altered to discharges via drainage tiles, with the exchange flows (water leaving and re-entering the open-channel via the stream bed and banks) being greatly decreased. Consequently, to improve nitrogen removal in these systems, these flows have to be intercepted or reestablished either through restoration of the old stream valley or through managed structures in the buffer strips.
|
|
