| 1. |
- Fornara, Andrea, et al.
(författare)
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Tailored Magnetic Nanoparticles for Direct and Sensitive Detection of Biomolecules in Biological Samples
- 2008
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Ingår i: Nano letters (Print). - : American Chemical Society (ACS). - 1530-6984 .- 1530-6992. ; 8:10, s. 3423-3428
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Tidskriftsartikel (refereegranskat)abstract
- We developed nanoparticles with tailored magnetic properties for sensitive detection of biomolecules directly in biological samples in a single step. Thermally blocked nanoparticles obtained by thermal hydrolysis are mixed with sample solutions and the variation of the magnetic relaxation due to surface binding is used to detect the presence of biomolecules. The binding events significantly increase the hydrodynamic volume of nanoparticles, thus changing their Brownian relaxation frequency which is measured by a specifically developed AC-susceptometer.The system was tested for the presence of Brucella antibodies in serum samples from infected cows and the surface of the nanoparticles was functionalized with lipopolysaccarides (LPS) from Brucella abortus. The hydrodynamic volume of functionalized particles increased by 25-35% as a result of the binding of the antibodies, as measured by changes in the susceptibility in an alternating magnetic field. The method has shown high sensitivity, with detection limit of 7 nmol·L-1 in serum without any pre-treatment of the biological samples.The detection method is very sensitive, cost-efficient and versatile, giving a direct indication if the animal is infected or not, making it suitable for point-of care applications. The functionalization of tailored magnetic nanoparticles can be modified to suit numerous homogenous assays for a wide range of applications.
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| 2. |
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| 3. |
- Johansson, Anna M., et al.
(författare)
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Detecting deletions in families affected by a dominant disease by use of marker data
- 2005
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Ingår i: Human Heredity. - : Karger. - 0001-5652 .- 1423-0062. ; 60:1, s. 26-35
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Tidskriftsartikel (refereegranskat)abstract
- A method of testing for whether inherited deletions are a cause of a single-locus dominant disease was derived, involving analysis of the marker segregation within the pedigree of a single family that segregates for the disease. It is shown that markers can be used to test deductively for the presence of an inherited deletion. The probabilities of confirming or rejecting the presence of a deletion in an arbitrary pedigree without inbreeding are then derived. The power of the test is shown to be limited in single trios but to increase rapidly as the size of the pedigree increases. For larger pedigrees, the probabilities of confirming or rejecting a deletion are higher than 0.9 for SNPs having a minor allele frequency greater than 0.4. The probabilities are higher using multiallelic markers such as microsatellites, reaching levels as high as 0.9 in even rather small pedigrees. In certain cases the test outcome is not deductive, a deletion being neither confirmed nor rejected. It is shown to still be possible then to employ a statistical test for the presence of a deletion by use of an a priori probability for a deletion.
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| 4. |
- Johansson, Christer, et al.
(författare)
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Extern redovisning
- 2009
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Bok (övrigt vetenskapligt/konstnärligt)abstract
- Extern redovisning behandlar allt från redovisningens grunder, sambandet mellan de olika finansiella rapporterna till beräkning av finansiella nycketal för interna och externa intressenter. I denna tredje upplaga har en omfattande revidering skett mot bakgrund av förändringar i lagar och regler. Dessutom har en större pedagogisk omstrukturering gjorts. I alla företag sker mängder av aktiviteter och dagligen fattas ett stort antal viktiga beslut. Bokens avsikt är att beskriva hur redovisningen kan tillhandahålla underlag för dessa beslut. Därigenom blir redovisning en viktig faktor för tillväxt och utveckling i hela samhällsekonomin.
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| 5. |
- Johansson, Christer, 1964, et al.
(författare)
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Externredovisning
- 2007
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Bok (övrigt vetenskapligt/konstnärligt)
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| 6. |
- Johansson, Rolf, et al.
(författare)
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Bokslutet från början
- 2008
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Bok (övrigt vetenskapligt/konstnärligt)abstract
- Bokslutet från början är en kombinerad fakta- och övningsbok som är en direkt fortsättning på grundboken i bokföring, Bokföring från början. I denna bok fördjupas kunskaperna. Steg för steg behandlas de olika områden man måste behärska för att kunna upprätta ett fullständigt bokslut med årsredovisning i enlighet med årsredovisningslagens krav.
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| 7. |
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| 8. |
- Lanke, E., et al.
(författare)
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Genetic analysis of 31 Swedish type 1 von Willebrand disease families reveals incomplete linkage to the von Willebrand factor gene and a high frequency of a certain disease haplotype
- 2005
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Ingår i: Journal of Thrombosis and Haemostasis. - : Wiley-Blackwell. - 1538-7933 .- 1538-7836. ; 3:12, s. 2656-2663
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Tidskriftsartikel (refereegranskat)abstract
- BACKGROUND: The most common type of von Willebrand disease (VWD), type 1, has in only a few cases been explained by an identified causative mutation in the von Willebrand factor (VWF) gene. The ABO blood group and other modifier loci outside the VWF gene may contribute to the development of type 1 VWD. OBJECTIVES AND METHODS: Our aim was to determine whether there was genetic linkage to the VWF gene in 31 Swedish type 1 VWD families. Stringent diagnostic criteria in accordance with ISTH guidelines were used. Genetic linkage was investigated by using two highly informative dinucleotide microsatellite markers, which we have recently identified, located in introns six and 15 of the VWF gene. We also investigated the existence of common disease haplotypes and the relation between type 1 VWD and ABO blood group. RESULTS: We found genetic linkage to the VWF gene in 27 (87%) of the families. However, in four (13%) of the families, there was clearly no genetic linkage. We found the 4751A>G (Tyr1584Cys) sequence variation in exon 28, which is a common mutation in the Canadian VWD population (14.3%), in only one of the 31 families (3.2%). A possible common mutation was identified in six of the 27 (22%) families with genetic linkage. Blood group O was over-represented among type 1 VWD patients. CONCLUSION: We conclude that there is linkage between the VWF gene and hereditary type 1 VWD in a majority of families.
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| 9. |
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| 10. |
- Abenius, Erik, et al.
(författare)
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Waveguide Truncation Using UPML in the Finite-Element Time-Domain Method
- 2005
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Rapport (övrigt vetenskapligt/konstnärligt)abstract
- An important part of numerical waveguide modeling is the termination of the waveguide using artificial boundaries. In this paper we develop a perfectly matched layer (PML) for waveguides in the finite-element time-domain method (FETD). The PML is discretized by prism elements aligned with the direction of propagation of the waveguide. Assuming that the waveguide is discretized by tetrahedra such a grid is easily generated from a cross-sectional surface in the waveguide. The proposed method has the advantage of being general with regard to the geometry and material of the waveguide. Previous works on PML for FETD have reported problems with late-time instability. While still present in the current approach, our results indicate that the instability is less severe for the prism element PML compared to a tetrahedral PML. Moreover, it may be controlled by increasing the number of grid points in the absorbing layer. It should be noted that the instability appears long after the energy has been attenuated and therefore pose no problems in practical computations. The performance of the suggested scheme is demonstrated for several waveguide problems, including an inhomogeneous case.
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