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| 2. |
- Bauer, Patrik Maximilian, et al.
(författare)
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Inflamed In Vitro Retina : Cytotoxic Neuroinflammation and Galectin-3 Expression
- 2016
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Ingår i: PLOS ONE. - : Public Library of Science (PLoS). - 1932-6203. ; 11:9
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Tidskriftsartikel (refereegranskat)abstract
- Background Disease progression in retinal neurodegeneration is strongly correlated to immune cell activation, which may have either a neuroprotective or neurotoxic effect. Increased knowledge about the immune response profile and retinal neurodegeneration may lead to candidate targets for treatments. Therefore, we have used the explanted retina as a model to explore the immune response and expression of the immune modulator galectin-3 (Gal-3), induced by the cultivation per se and after additional immune stimulation with lipopolysaccharide (LPS), and how this correlates with retinal neurotoxicity. Methods Post-natal mouse retinas were cultured in a defined medium. One group was stimulated with LPS (100 ng/ml, 24 h). Retinal architecture, apoptotic cell death, and micro- and macroglial activity were studied at the time of cultivation (0 days in vitro (DIV)) and at 3, 4 and 7 DIV using morphological staining, biochemical- and immunohistochemical techniques. Results Our results show that sustained activation of macro- and microglia, characterized by no detectable cytokine release and limited expression of Gal-3, is not furtherinducing apoptosis additional to the axotomy-induced apoptosis in innermost nuclear layer. An elevated immune response was detected after LPS stimulation, as demonstrated primarily by release of immune mediators (i.e. interleukin 2 (IL-2), IL-6, KC/GRO (also known as CLCX1) and tumour necrosis factor-α (TNF-α)), increased numbers of microglia displaying morphologies of late activation stages as well as Gal-3 expression. This was accompanied with increased apoptosis in the two additional nuclear layers, and damage to retinal gross architecture. Conclusion We demonstrate that an immune response characterized by sustained and increased release of cytokines, along with an increase in Gal-3 expression, is accompanied by significant increased neurotoxicity in the explanted retina. Further investigations using the current setting may lead to increased understanding on the mechanisms involved in neuronal loss in retinal neurodegenerations. © 2016 Bauer et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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| 3. |
- Johansson, Magdalena, 1984-, et al.
(författare)
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Physical activity and risk of first-time venous thromboembolism
- 2019
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Ingår i: European Journal of Preventive Cardiology. - : Sage Publications. - 2047-4873 .- 2047-4881. ; 26:11, s. 1181-1187
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Tidskriftsartikel (refereegranskat)abstract
- Background: Physical activity may have a protective effect against venous thromboembolism (VTE). The aim of this study was to investigate the association between leisure time physical activity, occupational physical activity, and the risk of VTE in men and women separately.Methods: The population-based, prospective Venous thromboEmbolism In Northern Sweden (VEINS) cohort study included 108,025 participants of health examinations between 1985 and 2014. Physical activity data were collected by questionnaire. Participants were followed from health examination to first-time VTE event, death, emigration or the end of the study. All VTE events were validated by reviewing medical records and radiology reports.Results: During 1,496,669 person-years, 2054 participants experienced VTE. Women who performed leisure time physical activity at least once a week had a lower risk of first-time VTE (hazard ratio (HR) 0.83; 95% confidence interval (CI) 0.71–0.98 after adjustments) compared with women with less or no physical activity. Furthermore, women with high occupational physical activity also had a lower risk of VTE (HR 0.85; 95% CI 0.74–0.98). In men, there was no consistent association between either measure of physical activity and the risk of VTE.Conclusion: We found an association between increased physical activity and a lower risk of first-time VTE in women.
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| 4. |
- Veje, Malin, et al.
(författare)
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Diagnosing tick-borne encephalitis: a re-evaluation of notified cases
- 2018
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Ingår i: European Journal of Clinical Microbiology and Infectious Diseases. - : Springer Science and Business Media LLC. - 0934-9723 .- 1435-4373. ; 37:2, s. 339-344
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Tidskriftsartikel (refereegranskat)abstract
- We set out to investigate the serological response of TBE virus (TBEV)-specific IgM and IgG antibodies in stored serum and cerebrospinal fluid (CSF) in notified TBE patients, in order to confirm or reject the diagnosis. We applied the ELISA methods used in clinical practice, Enzygnost and Immunozym, and assessed RT-PCR as a diagnostic tool. A total of 173 TBE cases were notified to the Public Health Agency. Samples from 129 patients were eligible for the study. Stored serum samples were found for 111 patients and CSF samples for 88 patients. All serum samples were analyzed with both Enzygnost and Immunozym, as well as an additional 140 control samples. CSF samples, including samples from ten controls, were analyzed with Immunozym. RT-PCR for TBEV was performed on 126 serum, two whole blood, 96 CSF, two feces and four nasopharynx samples. Only two of 111 notified patients lacked detectable TBEV IgM in serum, from whom one sample was RT-PCR positive. According to the ECDC definition, 117/129 (90.7%) of the reported TBE cases were confirmed. Positive RT-PCR results were obtained in eight patients, one from whole blood and eight from serum samples. Four out of eight of the RT-PCR positive patients were TBEV-IgM positive and none had detectable TBEV-specific IgG. All of the tested CSF, feces and nasopharynx samples were RT-PCR-negative. TBEV-specific IgG was detected in 88.4% and IgM in 31.6% of the CSF samples. RT-PCR on serum samples and CSF IgG antibodies can be used as complementary methods in TBE diagnostics, not least early in the disease course.
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| 5. |
- Weishaupt, Holger, et al.
(författare)
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Batch-normalization of cerebellar and medulloblastoma gene expression datasets utilizing empirically defined negative control genes
- 2019
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Ingår i: Bioinformatics. - : Oxford University Press. - 1367-4803 .- 1367-4811. ; 35:18, s. 3357-3364
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Tidskriftsartikel (refereegranskat)abstract
- Motivation: Medulloblastoma (MB) is a brain cancer predominantly arising in children. Roughly 70% of patients are cured today, but survivors often suffer from severe sequelae. MB has been extensively studied by molecular profiling, but often in small and scattered cohorts. To improve cure rates and reduce treatment side effects, accurate integration of such data to increase analytical power will be important, if not essential.Results: We have integrated 23 transcription datasets, spanning 1350 MB and 291 normal brain samples. To remove batch effects, we combined the Removal of Unwanted Variation (RUV) method with a novel pipeline for determining empirical negative control genes and a panel of metrics to evaluate normalization performance. The documented approach enabled the removal of a majority of batch effects, producing a large-scale, integrative dataset of MB and cerebellar expression data. The proposed strategy will be broadly applicable for accurate integration of data and incorporation of normal reference samples for studies of various diseases. We hope that the integrated dataset will improve current research in the field of MB by allowing more large-scale gene expression analyses.
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| 6. |
- Ablikim, M., et al.
(författare)
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Amplitude analysis of D+-> K-S(0)pi(+)pi(+)pi(-)
- 2019
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Ingår i: Physical Review D. - : AMER PHYSICAL SOC. - 2470-0010 .- 2470-0029. ; 100:7
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Tidskriftsartikel (refereegranskat)abstract
- The decay D+-> K-S(0)pi(+)pi(+)pi(-) is studied with an amplitude analysis using a data set of 2.93 fb(-1) of e(+)e(-) collisions at the psi(3770) peak accumulated by the BESIII detector. Intermediate states and nonresonant components, and their relative fractions and phases, have been determined. The significant amplitudes, which contribute to the model that best fits the data, are composed of five quasitwo-body decays K(S)(0)a(1)(1260)(+), (K) over bar (1)(1270)(0)pi(+) (K) over bar (1)(1400)(0)pi(+), (K) over bar (1)(1650)(0)pi(+), and (K) over bar (1460)(0)pi(+), a three-body decay K-S(0)pi(+)rho(0), as well as a nonresonant component K-S(0)pi(+)pi(+)pi(-). The dominant amplitude is K(S)(0)a(1)(1260)(+), with a fit fraction of (40.3 +/- 2.1 +/- 2.9)%, where the first and second uncertainties are statistical and systematic, respectively.
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| 7. |
- Ablikim, M., et al.
(författare)
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Complete Measurement of the Λ Electromagnetic Form Factors
- 2019
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Ingår i: Physical Review Letters. - 0031-9007 .- 1079-7114. ; 123:12
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Tidskriftsartikel (refereegranskat)abstract
- The exclusive process e(+)e(-) -> Lambda(Lambda) over bar, with Lambda -> p pi(-) and (Lambda) over bar -> (p) over bar pi(+), has been studied at root s = 2.396 GeV for measurement of the timelike Lambda electric and magnetic form factors, G(E) and G(M). A data sample, corresponding to an integrated luminosity of 66.9 pb(-1), was collected with the BESIII detector for this purpose. A multidimensional analysis with a complete decomposition of the spin structure of the reaction enables a determination of the modulus of the ratio R = vertical bar G(E)/G(M)vertical bar and, for the first time for any baryon, the relative phase Delta Phi = Phi(E) - Phi(M). The resulting values are R = 0.96 +/- 0.14(stat) +/- 0.02(syst) and Phi(F) = 37 degrees +/- 12 degrees (stat) +/- 6 degrees (syst), respectively. These are obtained using the recently established and most precise value of the asymmetry parameter alpha(Lambda) = 0.750 +/- 0.010 measured by BESIII. In addition, the cross section is measured with unprecedented precision to be sigma = 118.7 +/- 5.3(stat) +/- 5.1(syst) pb, which corresponds to an effective form factor of vertical bar G vertical bar = 0.123 +/- 0.003(stat) +/- 0.003(syst). The contribution from two-photon exchange is found to be negligible. Our result enables the first complete determination of baryon timelike electromagnetic form factors.
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| 8. |
- Ablikim, M., et al.
(författare)
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Cross section measurements of e+e- -> K+ K- K+ K- and ΦK+ K- at center-of-mass energies from 2.10 to 3.08 GeV
- 2019
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Ingår i: Physical Review D. - 2470-0010 .- 2470-0029. ; 100:3
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Tidskriftsartikel (refereegranskat)abstract
- We measure the Born cross sections of the process e(+)e(-) -> K+ K- K+ K- at center-of-mass (c.m.) energies, root s, between 2.100 and 3.080 GeV. The data were collected using the BESIII detector at the BEPCII collider. An enhancement at root s = 2.232 GeV is observed, very close to the e(+)e(-) -> Lambda(Lambda) over bar production threshold. A similar enhancement at the same c.m. energy is observed in the e(+)e(-) -> phi K+ K- cross section. The energy dependence of the K+ K- K+ K- and phi K+ K- cross sections differs significantly from that of e(+)e(-) -> phi pi(+)pi(-).
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| 9. |
- Ablikim, M., et al.
(författare)
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Cross section measurements of e(+) e(-) -> omega chi(c0) from root s=4.178 to 4.278 GeV
- 2019
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Ingår i: Physical Review D. - : AMER PHYSICAL SOC. - 2470-0010 .- 2470-0029. ; 99:9
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Tidskriftsartikel (refereegranskat)abstract
- The cross section of the process e(+)e(-) -> omega chi(C0) is measured at center -of -mass energies from root s = 4.178 to 4.278 GeV using a data sample of 7 fb(-1) collected with the BESIII detector operating at the BEPCII storage ring. The dependence of the cross section on root s shows a resonant structure with mass of (4218.5 +/- 1.6(stat) +/- 4.0(syst)) MeV/c(2) and width of (28.2 +/- 3.9(stat) +/- 1.6(syst)) MeV, respectively. This observation confirms and improves upon the result of a previous study. The angular distribution of the e(+) e(-) ->omega chi(C0) process is extracted for the first time.
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| 10. |
- Ablikim, M., et al.
(författare)
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First observation of the decay chi(cJ) -> Sigma(+)(p)over-barK(S)(0)+ c.c. (J=0,1,2)
- 2019
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Ingår i: Physical Review D. - : American Physical Society. - 2470-0010 .- 2470-0029. ; 100:9
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Tidskriftsartikel (refereegranskat)abstract
- Using E1 radiative transitions psi(3686) -> gamma chi(cJ) from a sample of (448.1 +/- 2.9) x 10(6) psi(3686) events collected with the BESIII detector, the decays chi(cJ) -> Sigma(+)(p) over barK(S)(0) + c.c.(J = 0,1,2) are studied. The decay branching fractions are measured to be B(chi(c0) -> Sigma(+)(p) over barK(S)(0) + c.c.) = (3.52 +/- 0.19 +/- 0.21) x 10(-4), B(chi(c1) -> Sigma(+)(p) over barK(S)(0) + c.c.) = (1.53 +/- 0.10 +/- 0.08) x 10(-4), and B(chi(c2) -> Sigma(+)(p) over barK(S)(0) + c.c.) = (8.25 +/- 0.83 +/- 0.49) x 10(-5), where the first and second uncertainties are the statistical and systematic ones, respectively. No evident intermediate resonances arc observed in the studied processes.
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