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Träfflista för sökning "WFRF:(Johansson Roger) srt2:(2000-2004)"

Sökning: WFRF:(Johansson Roger) > (2000-2004)

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  • Grenklo, Staffan, et al. (författare)
  • Anti-actin antibodies generated against profilin : actin distinguish between non-filamentous and filamentous actin, and label cultured cells in a dotted pattern
  • 2004
  • Ingår i: European Journal of Cell Biology. - : Elsevier BV. - 0171-9335 .- 1618-1298. ; 83:8, s. 413-423
  • Tidskriftsartikel (refereegranskat)abstract
    • Actin polymerization is a prominent feature of migrating cells, where it powers the protrusion of the leading edge. Many studies have characterized the well-ordered and dynamic arrangement of filamentous actin in this submembraneous space. However, less is known about the organization of unpolymerized actin. Previously, we reported on the use of covalently coupled profilin:actin to study actin dynamics and presented evidence that profilin-bound actin is a major source of actin for filament growth. To locate profilin:actin in the cell we have now used this non-dissociable complex for antibody generation, and obtained monospecific anti-actin and antiprofilin antibodies from two separate immunizations. Fluorescence microscopy revealed drastic differences in the staining pattern generated by the anti-actin antibody preparations. With one, distinct puncta appeared at the actin-rich leading edge and sometimes aligned with microtubules in the interior of the lamella, while the other displayed typical actin filament staining. Labelling experiments in vitro demonstrated failure of the first antibody to recognize filamentous actin and none of the two bound microtubules. The two anti-profilin antibodies purified in parallel generated a punctated pattern similar to that seen with the first anti-actin antibody. All antibody preparations labelled the nuclei.
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  • Johansson, Roger, 1966-, et al. (författare)
  • Assessing Flexibility : The Frequency and Horizon Approach
  • 2001
  • Konferensbidrag (övrigt vetenskapligt/konstnärligt)abstract
    • This paper focuses on flexibility in the area of flexible automatic assembly systems. Far-reaching research has been done in effort to develop flexible automatic assembly systems. A dilemma that appears in understanding the focus for these research projects is the immense amounts of flexibility concepts used. This paper proposes a structure for the concepts based on frequency and horizon for different events in an assembly system. The results are presented in a proposed “flexibility map”. Based on the “frequency and horizon approach” and the definition of flexibility as “the penalty of change” [1], an argumentation concerning what kind of flexibility is most valuable in an assembly system can be discussed. The paper also points out the demands for a flexibility that allows for de-coupling of high and low frequency events in a system. A clear distinction is made between dynamic flexibility and static flexibility. It is a well-known fact that the increase of the dynamic flexibility in an assembly system will decrease the static flexibility [2]. The paper ends with a proposal for decreasing the negative correlation between dynamic- and static flexibility. An objective for further research concerning whether a certain process within an assembly system affects certain flexibility is also presented.
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