| 1. |
- Roepstorff, P., et al.
(författare)
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Application of Plasma Desorption Mass Spectrometry to Molecular Weight Determination of Structural Protein from Insect Cuticle
- 1986
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Ingår i: Journal of Mass Spectrometry. - : Wiley. - 1076-5174 .- 1096-9888. ; 13:12, s. 689-691
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Tidskriftsartikel (refereegranskat)abstract
- During sequence determination of a structural protein from the cuticle of the migratory locust, Locusta migratoria, a discrepancy was found between the molecular weight calculated from the sequence data (15 323) and that estimated by SDS-gel electrophoresis (21 600). The protein contains several repeated sequences, and the discrepancy might indicate that part of the sequence was missing or that the protein contained a large prosthetic group. The molecular weight of the protein was determined by 127I-plasma desorption mass spectrometry to be 15 329±50 which confirms the sequence data and shows the molecular weight determination based on SDS-electrophoresis to be too high.
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| 2. |
- Jonsson, Gunnar, et al.
(författare)
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Compensation for Non-normal Ejection of Large Molecular Ions in Plasma Desorption Mass Spectrometry
- 1989
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Ingår i: Rapid Communications in Mass Spectrometry. - : Wiley. - 0951-4198 .- 1097-0231. ; 3:6, s. 190-191
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Tidskriftsartikel (refereegranskat)abstract
- By introducing an Einzel lens in the field-free region of a straight time-of-flight mass spectrometer, secondary ions of large mass can be detected more efficiently in plasma-desorption mass spectrometry. This procedure compensates for the large radial velocities of large molecular ions recently observed experimentally. It is shown that large molecular ions like those of lysozyme can now be detected more efficiently, and larger molecular ions than observed before in plasma-desorption mass spectrometry (like those of bovine ovalbumin, mol. wt 45 000) can now be studied.
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| 3. |
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