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Träfflista för sökning "WFRF:(Jordan H) srt2:(1995-1999)"

Sökning: WFRF:(Jordan H) > (1995-1999)

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  • Feuk-Lagerstedt, Elisabeth, 1949, et al. (författare)
  • Identification of CD66a and CD66b as the major galectin-3 receptor candidates in human neutrophils.
  • 1999
  • Ingår i: Journal of immunology (Baltimore, Md. : 1950). - 0022-1767. ; 163:10, s. 5592-8
  • Tidskriftsartikel (refereegranskat)abstract
    • The mammalian lectin galectin-3 is a potent stimulus of human neutrophils, provided that the receptor(s) for the lectin has been mobilized to the cell surface before activation. We have recently shown that the receptors for galectin-3 are stored in intracellular mobilizable granules. Here we show supportive evidence for this in that DMSO-differentiated (neutrophil-like) HL-60 cells, which lack gelatinase and specific granules, are nonresponsive when exposed to galectin-3. Neutrophil granules were subsequently used for isolation of galectin-3 receptors by affinity chromatography. Proteins eluted from a galectin-3-Sepharose column by lactose were analyzed on SDS-polyacrylamide gels and showed two major bands of 100 and 160 kDa and a minor band of 120 kDa. By immunoblotting, these proteins were shown to correspond to CD66a (160 kDa), CD66b (100 kDa), and lysosome-associated membrane glycoprotein-1 and -2 (Lamp-1 and -2; 120 kDa). The unresponsive HL-60 cells lacked the CD66 Ags but contained the Lamps, implying that neutrophil CD66a and/or CD66b may be the functional galectin-3 receptors. This conclusion was supported by the subcellular localization of the CD66 proteins to the gelatinase and specific granules in resting neutrophils.
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  • Grillner, S, et al. (författare)
  • Vertebrate locomotion--a lamprey perspective
  • 1998
  • Ingår i: Annals of the New York Academy of Sciences. - : Wiley. - 0077-8923 .- 1749-6632. ; 860, s. 1-18
  • Tidskriftsartikel (refereegranskat)
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