| 1. |
- Moing, A, et al.
(författare)
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Quantitative metabolic pro. ling by 1-dimensional H-1-NMR analyses : application to plant genetics and functional genomics
- 2004
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Ingår i: Functional Plant Biology. - : CSIRO PUBLISHING. - 1445-4408 .- 1445-4416. ; 31:9, s. 889-902
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Tidskriftsartikel (refereegranskat)abstract
- Metabolic profiling by 1-dimensional (1-D) H-1-nuclear magnetic resonance (NMR) was tested for absolute quanti. cation of soluble sugars, organic acids, amino acids and some secondary metabolites in fruit, roots and leaves. The metabolite responsible for each peak of the H-1-NMR spectra was identified from spectra of pure compounds. Peak identity was confirmed by the addition of a small amount of commercially-available pure substance. H-1-NMR spectra acquisition was automated. H-1-NMR absolute quantification was performed with a synthesised electronic reference signal and validated by comparison with enzymatic or HPLC analyses; the correlation coefficients between H-1-NMR data and enzymatic or HPLC data were highly significant. Depending on the species and tissues, 14 - 17 metabolites could be quantified with 15 - 25 min acquisition time. The detection limit was approximately 1 - 9 mug in the NMR tube, depending on the compound. Quantitative data were used for ( 1) a genetic study of strawberry fruit quality, ( 2) a functional study of tomato transformants overexpressing hexokinase and ( 3) a study of Arabidopsis phosphoenolpyruvate carboxylase transformants with several lines showing decreased activity of the enzyme. Biochemical phenotyping of the fruits of a strawberry offspring allowed the detection of quantitative trait loci (QTL) controlling fruit quality. Comparison of the roots of wild types and hexokinase tomato transformants using principal component analysis of metabolic profiles revealed that environmental factors, i.e. culture conditions, can significantly modify the metabolic status of plants and thus hide or emphasise the expression of a given genetic background. The decrease in phosphoenolpyruvate carboxylase activity ( up to 75%) in Arabidopsis transformants impacted on the metabolic profiles without compromising plant growth, thus supporting the idea that the enzyme has a low influence on the carbon flux through the anaplerotic pathway.
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| 2. |
- Denoyes-Rothan, B, et al.
(författare)
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QTL analysis for resistances to Colletotrichum acutatum and Phytophthora cactorum in octoploid strawberry (Fragaria x ananassa)
- 2004
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Ingår i: Proceedings of the XIth Eucarpia Symposium on Fruit Breeding and Genetics, Vols 1 and 2. - : INTERNATIONAL SOCIETY HORTICULTURAL SCIENCE. ; , s. 147-151
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Konferensbidrag (refereegranskat)abstract
- Colletotrichum acutatum and Phytophthora cactorum are two of the most important pathogens of strawberry in Europe, and particularly in France. The characterization of quantitative trait loci (QTL) controlling resistances to these two pathogens would allow a better knowledge of the genetic architecture of these resistances in order to develop durable resistance. A pseudo-testcross strawberry population derived from the cross between Capitola and CF1116 was used for detecting QTLs associated with C. acutatum and P cactorum resistances. One hundred and eighty five individuals were inoculated with C. acutatum by dipping cold stored plants in a conidial suspension adjusted to 2.10(6) conidia per ml. They were also inoculated separately with P cactorum by inserting an agar disk containing mycelium into the crown. Each inoculation was conducted twice. Putative QTLs for resistance to C acutatum and P cactorum were identified by Interval Mapping (Mapmaker-QTL) using a LOD threshold of 2.
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| 3. |
- Lerceteau-Köhler, Estelle, et al.
(författare)
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Characterization of mixed disomic and polysomic inheritance in the octoploid strawberry (Fragaria x ananassa) using AFLP mapping
- 2003
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Ingår i: Theoretical and Applied Genetics. - : SPRINGER-VERLAG. - 0040-5752 .- 1432-2242. ; 107:4, s. 619-628
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Tidskriftsartikel (refereegranskat)abstract
- A two-way pseudo-testcross strategy, combined with Single Dose Restriction Fragment (SDRF) marker analysis, was used for genetic mapping in the octoploid cultivated strawberry Fragaria x ananassa (2n=8x=56). Based on a 113 full-sib progeny from a cross between the variety Capitola and the clone CF1116, we generated two parental maps using Amplified Fragment Length Polymorphism (AFLP) markers. Ninety two percent of the markers (727 out of 789) showed ratios corresponding to simplex markers (the majority being SDRF markers), and 8% (62 out of 789) fitted a multiplex ratio. Linkage maps were first established using SDRF markers in coupling phase. The female map comprised 235 markers distributed among 43 co-segregation groups, giving a map size of 1,604 cM. On the male map, 280 markers were assigned to 43 co-segregation groups, yielding a map size of 1,496 cM. Once the co-segregation groups were established, their association was tested using repulsion-phase markers. In total, taking into account associations representing the same linkage groups, 30 linkage groups were detected on the female side and 28 on the male side. On the female map, 68.3% of the pairwise marker linkages were in coupling versus 31.7% in repulsion phase, and the corresponding figures on the male map were 72.2% and 27.8%, respectively. In addition, both groups linked only in the coupling phase and groups linked in the repulsion phase were characterized. The observations suggest that the meiotic behavior of the F. x ananassa genome is neither fully disomic nor fully polysomic, but rather mixed. The genome may not be as completely diploidized as previously assumed.
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| 4. |
- Lerceteau-Köhler, Estelle, et al.
(författare)
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Development of a SCAR marker linked to dominant gene conferring resistance to Colletotrichum acutatum in strawberry (Fragaria x ananassa)
- 2003
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Ingår i: BERRY CROP BREEDING, PRODUCTION AND UTILIZATION FOR A NEW CENTURY. - : INTERNATIONAL SOCIETY HORTICULTURAL SCIENCE. ; , s. 85-91
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Konferensbidrag (refereegranskat)abstract
- Colletotrichum acutatum is responsible for strawberry anthracnose, one of the main disease of cultivated strawberry in the south-western area of France (60% of the French production). Anthracnose is a polycyclic disease for which no efficient disease control method has been developed. Therefore, the development of cultivars with sustainable resistance, i.e. efficient against the two pathogenicity groups, may be promising to lower both the level of damage and the use of chemicals. The objective of this study is to identify molecular markers linked to the major dominant gene conferring resistance to pathogenicity group 2 by using an approach of Bulk Segregant Analysis (BSA) and Amplified Fragment Length Polymorphisin (AFLP) markers. We identified four molecular markers, named mf1 to mf4, linked to the resistance gene to the pathogenicity group 2 of C. acutatum. Within a segregating population (back cross type) for the dominant gene, no recombination between the resistance allele and the marker mf3 was detected in 78 BC1 individuals studied. One Sequence Characterized Amplified Region (SCAR) obtained from mf4 (SCAR-mf4) provided a fast and easy test for selection of anthracnose resistant plants at early stage, with a misidentification rate of 1.3%. The evaluation of this SCAR marker in few strawberry varieties showed that only some resistant varieties possessed this marker.
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| 5. |
- Lerceteau-Köhler, Estelle, et al.
(författare)
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QTL analysis for fruit quality traits and resistance to Colletotrichum acutatum and Phytophthora cactorum in octoploid strawberry (Fragaria x ananassa)
- 2004
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Ingår i: PROCEEDINGS OF THE EURO BERRY SYMPOSIUM - COST 836 FINAL WORSKHOP. - : INT SOC HORTICULTURAL SCIENCE. ; , s. 93-97
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Konferensbidrag (refereegranskat)abstract
- Mapping of quantitative trait loci (QTL) controlling components of strawberry (Fragaria x ananassa Duch., 2n=8x=56) fruit quality and resistance to Colletotrichum acutatum and Phytophthora cactorum can be used to provide a better understanding of the genetic control of the traits, and to develop marker assisted selection for breeders. A segregating population of 213 individuals of a cross between ‘Capitola’ and ‘CF1116’, two genotypes with many contrasting fruit quality traits, was used for genetic mapping. ‘Capitola’ was resistant to the C. acutatum pathogenicity group 1 (pg1) and to P. cactorum while ‘CF1116’ was susceptible to C. acutatum and moderately susceptible to P. cactorum. Fruit quality was evaluated on a total of 34 traits, including developmental and fruit aspect related traits, texture related traits, fruit acidity, sugars, ascorbate and amino acid concentrations. Most of the traits except the amino acid concentrations were evaluated during two successive years. Disease responses to C. acutatum (pg2) and P. cactorum were evaluated using a scale from 0 (no observed symptom) to 5 (dead plant) in two successive independent experiments. Data were analysed for putative QTLs using MapQTL. For the fruit quality traits, a total of 22 significant QTLs were detected by simple interval mapping (LOD>3.0) in the first year of analyses and 17 were detected in the second year. Only two QTLs could be detected in both years. The percentages of phenotypic variance explained by each QTL ranged from 6.5% to 16.0%. For resistances, a total of nine QTLs was detected (LOD>2.0), 4 for resistance to C acutatum and 5 for resistance to P. cactorum with the percentage of variation explained by a QTL ranging from 6.5 to 12.2%. No QTL for the P. cactorum resistance overlapped the QTLs for the C acutatum resistance.
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| 6. |
- Lerceteau-Köhler, Estelle, et al.
(författare)
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QTL analysis for fruit quality traits in octoploid strawberry (Fragaria x ananassa)
- 2004
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Ingår i: PROCEEDINGS OF THE XITH EUCARPIA SYMPOSIUM ON FRUIT BREEDING AND GENETICS, VOLS 1 AND 2. - : INT SOC HORTICULTURAL SCIENCE. ; , s. 331-335
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Konferensbidrag (refereegranskat)abstract
- Mapping of quantitative trait loci (QTL) controlling components of strawberry (Fragaria x ananassa Duch., 2n=8x=56) fruit quality can be used to provide a better understanding of their genetic control, and to develop marker assisted selection for breeders. For this purpose, a segregating population of 213 individuals of a cross between ‘Capitola’ and CF1116, two genotypes with many contrasting fruit quality traits, was used for genetic mapping. A total of 34 traits involved in fruit quality were evaluated, including developmental and fruit aspect related traits, texture related traits, fruit acidity, sugar and ascorbate concentrations. Amino acid concentrations were quantified using one-dimensional proton NMR spectroscopy. Most of the traits except the amino acid concentrations were evaluated during two successive years. A total of 22 significant QTLs were detected by simple interval mapping (LOD > 3.0) in year 1, four on the female map and 18 on the male map, whereas 17 were detected in year 2, ten on the female map and seven on the male map. Only two QTLs could be detected in both years. When removing the year effect, 22 QTLs were observed, eight on the female and 14 on the male map. The percentages of phenotypic variance explained by each QTL ranged from 6.5% to 16.0%.
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| 7. |
- Lerceteau-Köhler, Estelle, et al.
(författare)
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The use of molecular markers for durable resistance breeding in the cultivated strawberry (Fragaria x ananassa)
- 2002
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Ingår i: PROCEEDINGS OF THE FOURTH INTERNATIONAL STRAWBERRY SYMPOSIUM, VOLS 1 AND 2. - : INTERNATIONAL SOCIETY HORTICULTURAL SCIENCE. ; , s. 615-618
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Konferensbidrag (refereegranskat)abstract
- In France, particularly in the southwestern area, most damage to the cultivated strawberry is anthracnose, caused by Colletotrichum acutatum. Anthracnose is a polycyclic disease for which no satisfactory disease control method has been developed. Therefore, the objective of our project is to construct durable resistance by combining the different genetic components of resistance to lower both the level of damage and the use of chemicals. Genetic components will be selected by using molecular markers linked to these loci. For monogenic resistance to the pathogenicity group 2 of C. acutatum, bulk segregant analysis (BSA) combined with the AFLP markers has been developed. Four markers were found linked to the dominant gene with a recombination percentage ranging from 0 to 13.9%. For complex resistance to the pathogenicity group 1 of C. acutatum, a linkage map has been constructed in order to detect putative QTLs associated with this complex resistance. The female map was comprised of 31 SDRF (Single Dose Restriction Fragment) markers assigned to 11 linkage groups giving a total size of 392.9 cM. On the male map, 52 SDRF markers mapped on 17 linkage groups giving a total size of 406.9 cM. The work is being continued with the addition of more markers on the maps.
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| 8. |
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| 9. |
- Yin, T M, et al.
(författare)
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Nearly complete genetic maps of Pinus sylvestris L. (Scots pine) constructed by AFLP marker analysis in a full-sib family
- 2003
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Ingår i: Theoretical and Applied Genetics. - : SPRINGER. - 0040-5752 .- 1432-2242. ; 106:6, s. 1075-1083
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Tidskriftsartikel (refereegranskat)abstract
- We have constructed nearly complete linkage maps of Pinus sylvestris (L.) using AFLP markers based on a two-way pseudo-testcross strategy in a full-sib family founded in an advanced breeding program. With 39 primer combinations, a total of 737 markers (320 from the mother and 417 froth the father) segregated in a 1:1 ratio, corresponding to DNA polymorphism: heterozygous in one parent and null in the other. In the maternal parent, 188 framework markers were mapped in 12 linkage groups, equivalent to the Pinus haploid chromosome number, with a total coverage of 1,695.5 cM. In the paternal parent, 245 framework markers established a map with 15 linkage groups, spanning a genome length of 1,718.5 cM. The estimated total map length was (L) over cap (F) = 1, 681 cM for the female and (L) over cap (M) = 1, 645 cM for the male using a modified method-of-moment estimator. Combining these values with those estimated from the observed map lengths in both parents, we estimated the genome length in Scots pine to be between 1,600 and 2,100 cM. Our genome coverage was estimated to be more than 98% with a framework marker interval of 20 cM for both parents. Most of the female and male linkage groups were associated through the analysis of the intercross markers.
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