| 1. |
- Byröd, Martin, et al.
(författare)
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Fast Optimal Three View Triangulation
- 2007
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Ingår i: Lecture Notes in Computer Science. - Berlin, Heidelberg : Springer Berlin Heidelberg. - 9783540763895 ; 4844, s. 549-559
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Konferensbidrag (refereegranskat)abstract
- We consider the problem of $L_2$-optimal triangulation from three separate views. Triangulation is an important part of numerous computer vision systems. Under gaussian noise, minimizing the $L_2$ norm of the reprojection error gives a statistically optimal estimate. This has been solved for two views. However, for three or more views, it is not clear how this should be done. A previously proposed, but computationally impractical, method draws on Gr{"o}bner basis techniques to solve for the complete set of stationary points of the cost function. We show how this method can be modified to become significantly more stable and hence given a fast implementation in standard IEEE double precision. We evaluate the precision and speed of the new method on both synthetic and real data. The algorithm has been implemented in a freely available software package which can be downloaded from the Internet.
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| 2. |
- Jo, Ji Song, et al.
(författare)
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Anti-wrinkle activity of beta-carotene extracted & purified from recombinant Escherichia coli
- 2008
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Ingår i: KSBB Journal. - 1225-7117 .- 2288-8268. ; 23:6, s. 513-518
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Tidskriftsartikel (refereegranskat)abstract
- This paper described the extraction/purification of β-carotene from recombinant E.coli and evaluation of anti-wrinkle activity of purified β-carotene. No significant differences in extraction yields were observed when hexane or isobutyl acetate was used. However, extraction from wet-cell cake resulted in 2-fold higher amount of β-carotene than that from dry cells. Disruption of 5 g-wet cells by ultrasonic homogenizer, acetone dehydration, extraction with isobutyl acetate resulted in 36 mg of β-carotene corresponding to 61.2% of recovery. The formation and separation of ββ-carotene crystal improved the purity. 633 mg of β-carotene crystal with 93% purity was obtained from 223 g/L of wet-cell cake harvested from 2.5-L fed-batch culture broth. The cultures of normal human primary fibroblast were performed to investigate the effect of β-carotene on cytotoxicity as MTT assay and anti-wrinkle activity as collagen synthesis assays. 1.7μM of β-carotene was found to be optimal concentration at which 1.4-fold higher amount of collagen was synthesized than that in absence of β-carotene. This indicates that highly purified β-carotene can be obtained from recombinant E.coli by applying simple method with less toxic solvent and can be used in functional cosmetics as anti-wrinkle agent.
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