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Träfflista för sökning "WFRF:(Karl Z.) srt2:(2000-2004)"

Sökning: WFRF:(Karl Z.) > (2000-2004)

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1.
  • Wu, Z, et al. (författare)
  • Distinct effects of Vibrio cholerae haemagglutinin/protease on the structure and localization of the tight junction-associated proteins occludin and ZO-1
  • 2000
  • Ingår i: Cellular Microbiology. - : Hindawi Limited. - 1462-5814 .- 1462-5822. ; 2:1, s. 11-17
  • Tidskriftsartikel (refereegranskat)abstract
    • Vibrio cholerae produces a little-studied cytotoxin, haemagglutinin/protease (HA/P), in addition to several better-characterized enterotoxins, i.e. cholera toxin (CT), zonula occludens toxin (ZOT) and accessory cholera enterotoxin (Ace). We have found recently that HA/P perturbs the barrier function of Mardin-Darby canine kidney epithelial cell line (MDCK-I) by affecting the intercellular tight junctions (TJs) and the F- actin cytoskeleton. In the present study we have assessed more specifically how TJs are affected by HA/P by investigating the cellular localization and biochemical integrity of two well-characterized TJ-associated proteins, occludin and ZO-1. Western blot analysis showed that occludin bands of 66-85 kDa were digested by HA/P to two predominant bands of around 50 kDa and 35 kDa, and that this degradation was greatly attenuated when the specific bacterial metalloproteinase inhibitor Zincov was co-administered. Trypsin, on the other hand, did not degrade occludin when it was applied in the same way, suggesting that the degradation of occludin by HA/P is an early and specific event. The other TJ-associated protein ZO-1 was not degraded by HA/P in parallel experiments, suggesting the selectivity of HA/P-associated protein degradation. Moreover, immunofluorescence labelling and confocal microscopy showed that ZO-1, but not occludin, around cell-cell boundaries was rearranged by HA/P treatment. Since ZO-1 is located on the inside of the plasma membrane and is directly associated with occludin, the results indicate that breakdown of occludin may send signals to ZO-1 that affect its organization and the structure of the F-actin cytoskeleton. Our finding that the zinc-containing metalloprotease of V. cholerae specifically degraded occludin suggests that specific degradation of important host proteins by bacterial zinc-containing metalloproteases may be an important mechanism in microbial pathogenesis.
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2.
  • Zozoulenko, Igor, et al. (författare)
  • Magnetoconductance of a few-electron open quantum dot
  • 2000
  • Ingår i: Physica. E, Low-Dimensional systems and nanostructures. - 1386-9477 .- 1873-1759. ; 6:1, s. 409-413
  • Tidskriftsartikel (refereegranskat)abstract
    • Magnetoconductance of a small open lateral dot is studied both theoretically and experimentally for the conditions when the dot contains down to approximately 15 electrons. We confirm the existence of a new regime for open dots in which the transport through the structure occurs through individual eigenstates of the corresponding closed dot. In particular, at low magnetic fields the characteristic features in the conductance are related to the underlying eigenspectrum shells. When the number of modes in the leads is reduced more detailed structures within the shells due to single eigenlevels becomes discernible. At higher fields Landau level condensation is evident as well as the crossing of levels collapsing to the different Landau levels.
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