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- Dahlgren, Claes, 1949, et al.
(författare)
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Respiratory burst in human neutrophils.
- 1999
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Ingår i: Journal of immunological methods. - 0022-1759. ; 232:1-2, s. 3-14
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Forskningsöversikt (refereegranskat)abstract
- During phagocytosis of microbial intruders, professional phagocytes of our innate immune system increase their oxygen consumption through the activity of an NADPH-oxidase that generates superoxide anion (O(2)(-)) and hydrogen peroxide (H(2)O(2)). These oxygen metabolites give rise to yet other reactive oxygen species that are strongly anti-microbial but which may also cause damage by destructing surrounding tissue and inducing apoptosis in other immune reactive cells. The development of methodology to measure the generation/release of phagocyte respiratory burst products is thus of great importance, and a number of different techniques are currently in use for this purpose. Three of the techniques that we have used, (luminol/isoluminol amplified chemiluminescence, cytochrome C reduction, and PHPA oxidation technique) are described in more detail in this review. We hope to convince the readers that these techniques are valuable tools in basic as well as more clinically oriented research dealing with phagocyte function. The basic principles for luminol/isoluminol-amplified chemiluminescence is used as the starting point for discussing methodological problems related to measurements of oxygen metabolites generated by professional phagocytes.
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- Dahlgren, Claes, 1949, et al.
(författare)
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The lysosomal membrane glycoproteins Lamp-1 and Lamp-2 are present in mobilizable organelles, but are absent from the azurophil granules of human neutrophils.
- 1995
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Ingår i: The Biochemical journal. - 0264-6021. ; 311 ( Pt 2), s. 667-74
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Tidskriftsartikel (refereegranskat)abstract
- The subcellular localization of two members of a highly glycosylated protein group present in lysosomal membranes in most cells, the lysosome-associated membrane proteins 1 and 2 (Lamp-1 and Lamp-2), was examined in human neutrophil granulocytes. Antibodies that were raised against purified Lamp-1 adn Lamp-2 gave a distinct granular staining of the cytoplasm upon immunostaining of neutrophils. Subcellular fractionation was used to separate the azurophil and specific granules from a light-membrane fraction containing plasma membranes and secretory vesicles, and Western blotting was used to determine the presence of the Lamps in these fractions. The results show that Lamp-1 and Lamp-2 are present in the specific-granule-enriched fraction and in the light-membrane fraction, but not in the azurophil granules. Separation of secretory vesicles from plasma membranes disclosed that the light-membrane Lamps were present primarily in the secretory-vesicle-enriched fraction. During phagocytosis both Lamp-1 and Lamp-2 became markedly concentrated around the ingested particle and they both appear on the cell surface when the secretory organelles are mobilized.
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- Edvardsson, Bo, et al.
(författare)
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Quality Maps : a way of working with quality development
- 1997
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Rapport (övrigt vetenskapligt/konstnärligt)abstract
- Quality Maps - a way of working with quality developmentMunicipal residents have the right to demand the best possible service for the taxes that they pay, and who better to judge municipal services than those who use the service? This is the starting point for the work procedure presented in this book. We explain how modern quality thought can be applied to municipal operations with the help of quality maps; simple aids to be used in conjunction with quality development. The subject matter also includes quality issues in connection with management by objectives, follow up and dialogue between elected representatives and municipal residents and employees.The book is based on experience of work with quality development in general service production which is carried out at The Service Research Center, Karlstad, Sweden. The examples are founded on work centred on the development of efficient control systems primarily in the areas of leisure and culture within The Municipality of Norrköping.-Kvalitetskartor - ett sätt att arbeta med kvalitetsutvecklingKommuninnevånarna har rätt att kräva bästa möjliga service för de skatter de betalar och vem är bättre på att bedöma kvaliteten i den kommunala servicen än de som använder den? Detta är utgångspunkten för det arbetssätt som presenteras i denna skrift och som visar hur det moderna kvalitetstänkandet kan ges en väg in i den kommunala verksamheten. Här presenteras kvalitetskartor som ett enkelt hjälpmedel i arbetet med kvalitetsutveckling. Samtidigt sätter skriften in kvalitetsfrågor i ett sammanhang där också målstyrning, resultatuppföljning och dialog mellan förtroendevalda, invånare och personal ingår.Skriften bygger på erfarenheter från det arbete med kvalitetsutveckling inom allmän tjänsteproduktion som bedrivits av Centrum för tjänsteforskning - CTF i Karlstad. Exemplen är hämtade från arbetet med att utveckla fungerande styrformer inom framför allt kultur- och fritidsområdet i Norrköpings kommun.Bo Edvardsson är biträdande professor och chef för Centrum för tjänsteforskning - CTF vid Högskolan i Karlstad. Peter Karlsson är beställare inom området kultur och fritid inom Norrköpings kommun. Anna Lindström arbetar vid Utvecklingssektionen vid Svenska Kommunförbundet
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