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- Bosa Ochoa, Carlos Felipe, et al.
(författare)
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Respuesta de Tecia solanivora (Lepidoptera: Gelechiidae) a compuestos volátiles de papa, Solanum tuberosum
- 2011
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Ingår i: Revista Colombiana De Entomologia. - 0120-0488. ; 37, s. 1-7
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Tidskriftsartikel (refereegranskat)abstract
- Trampas cebadas con volátiles de plantas como fuente de atrayente pueden ser una herramienta importante para el monitoreo o control de insectos plaga. La polilla guatemalteca,Teciasolanivora, es una plaga limitante en el cultivo de la papa y aún se desconoce su relación con su planta hospedera, la papa. Se estudió el efecto que producen olores de las diferentes estructuras de la planta hospedera en el comportamiento del insecto. También se estudiaron los compuestos metilfenilacetato y sulcatón, dos compuestos volátiles de la planta identificados previamente que son emitidos por las flores y los tubérculos, además de producir respuestas antenales de T.solanivora. Se realizaron ensayos en olfatómetro y en una jaula recubierta con tul con la utilización de trampas de captura. En olfatómetro la estructura que más atrajo a las hembras fue la flor. En la jaula se registró una mayor captura de hembras en trampas cebadas con el compuesto metilfenilacetato a 100μg, y no se observó un efecto sinérgico al combinar los dos compuestos. Los resultados sugieren la evaluación de otros compuestos sintéticos de la planta de papa y sus mezclas para profundizar en el comportamiento de este insecto.
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| 2. |
- Dejene Biasazin, Tibebe, et al.
(författare)
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Identification of Host Blends that Attract the African Invasive Fruit Fly, Bactrocera invadens
- 2014
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Ingår i: Journal of Chemical Ecology. - : Springer Science and Business Media LLC. - 0098-0331 .- 1573-1561. ; 40, s. 966-976
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Tidskriftsartikel (refereegranskat)abstract
- Bactrocera invadens, an invasive fruit fly species in the Afro-tropical region belonging to the Bactrocera dorsalis complex, causes considerable damage to fruit production and productivity. We sought to find attractants from hosts of B. invadens that could serve as baits in traps for monitoring and management of this pest. The attractiveness of volatiles from four different fruit species (mango, guava, banana and orange) at two stages of ripeness (ripe or unripe) was tested in an olfactometer assay. All fruits were attractive against a clean air control. Using hexane extracts of volatile collections of fruits, we demonstrated that male flies preferred the volatiles of ripe guava and orange over unripe fruit extracts. There was a slight difference in preference between females and males; females preferred orange to guava and mango, whereas males preferred mango and guava to orange. Gas chromatography/electroantennographic detection (GC/EAD) and GC/mass spectrometry (GC/MS) were used to identify compounds to which B. invadens antennae were sensitive. GC/EAD recordings from distal and medio-central parts of the fly antenna showed responses to a number of compounds from each fruit species, with esters dominating the responses. Synthetic blends were made for each fruit species using the shared antennally active compounds in ratios found in the extracts. In the olfactometer, B. invadens was most attracted to the banana and orange blends, followed by the mango and guava blends. The synthetic banana blend was as attractive as the volatile collection of banana, although both were less attractive than the fruit. The results demonstrate that composing attractive blends from GC/EAD-active constituents shared by host fruits can be effective for formulating attractive synthetic host mimics for generalist fruit fly species, such as B. invadens.
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| 3. |
- Karlsson, Miriam Frida, et al.
(författare)
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Guatemalan potato moth Tecia solanivora distinguish odour profiles from qualitatively, different potatoes Solanum tuberosum L.
- 2013
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Ingår i: Phytochemistry. - : Elsevier BV. - 0031-9422 .- 1873-3700. ; 85, s. 72-81
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Tidskriftsartikel (refereegranskat)abstract
- Guatemalan potato moth, Tecia solanivora, lay eggs in the soil nearby potato Solanum spp. and larvae feed on the tubers. We investigated the oviposition behaviour of T. solanivora females and the survival of larval offspring on healthy vs. stressed, i.e. light exposed and/or damaged potato tubers. In choice tests, females laid significantly more eggs in response to potato odour of healthy tubers and female oviposition preference correlated with higher larval survival. Survival of larvae was negatively correlated with the tuber content of the steroid glycoalkaloids alpha-solanine and alpha-chaconine: healthy potatoes contained lower amounts than stressed tubers, ranging from 25 to 500 mu g g(-1) and from 30 to 600 mu g g(-1), respectively. Analysis of volatile compounds emitted by potato tubers revealed that stressed tubers could clearly be distinguished from healthy tubers by the composition of their volatile profiles. Compounds that contributed to this difference were e.g. decanal, nonanal, isopropyl myristate, phenylacetaldehyde, benzothiazole, heptadecane, octadecane, myristicin, E,E-alpha-famesene and verbenone. Oviposition assays, when female moths were not in contact with the tubers, clearly demonstrated that volatiles guide the females to lay fewer eggs on stressed tubers that are of inferior quality for the larvae. We propose that volatiles, such as sesquiterpenes and aldehydes, mediate oviposition behaviour and are correlated with biosynthetically related, non-volatile compounds, such as steroidal glycoalkaloids, which influence larval survival. We conclude that the oviposition response and larval survival of T. solanivora on healthy vs. stressed tubers supports the preference performance hypothesis for insect herbivores. (C) 2012 Elsevier Ltd. All rights reserved.
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| 4. |
- Karlsson, Miriam Frida, et al.
(författare)
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Mating Disruption of Guatemalan Potato Moth Tecia Solanivora by Attractive and Non-Attractive Pheromone Blends
- 2012
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Ingår i: Journal of Chemical Ecology. - : Springer Science and Business Media LLC. - 0098-0331 .- 1573-1561. ; 38, s. 63-70
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Tidskriftsartikel (refereegranskat)abstract
- The behavioral mechanisms of mating disruption in Guatemalan potato moth Tecia solanivora were studied using the sex pheromone components, (E)-3-dodecenyl acetate, (Z)-3-dodecenyl acetate, and dodecyl acetate, formulated in a 100:1:20-ratio mimicking the female-produced blend, and in a 100: 56: 100 off-blend ratio. The mode of action of these two blends was tested in mating disruption experiments in the field and in a greenhouse, as well as in a laboratory wind tunnel. Field treatments with both blends at 80 g pheromone per ha reduced male attraction to trap lures baited with 100 mu g of female sex pheromone. In mesh-house treatments, these two blends were equally effective at reducing male attraction to traps baited with live females and mating of caged females. Subsequent flight tunnel tests corroborated that both blends reduced attraction of naive males to calling females, and pre-exposure of males with either dispenser blend for 24 hr resulted in a strongly reduced response to calling females. The pre-exposure effect was reversible, with males again responsive after 24 hr in clean air. The two dispenser formulations produced a similar effect on male behavior, despite the differences in blend composition. One mating disruption dispenser formulated with either the female-blend or off-blend elicited the same rate of male upwind attraction in a wind-tunnel bioassay. Sensory overload and camouflage, therefore, are contributing mechanisms to mating disruption using either blend. The off-blend, which is more economical to synthesize, is a valuable tool for further development of mating disruption against this major pest of potatoes in Latin America.
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| 5. |
- Karlsson, Miriam Frida
(författare)
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Odours, potato and insects : host finding and reproduction behaviour
- 2010
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Rapport (övrigt vetenskapligt/konstnärligt)abstract
- Plant odours can give important information about the specie and these emitted chemical messengers mediate host-finding behaviour, to the insects living on potato. During the development of the potato crop, lasting approximately tree months, the insects described in this paper, has to find the crop. They then chose a part of the potato; leaves, tubers or flowers, where they feed, hide, mate or oviposit. Host plant selection or host preference is not only governed by nutritional quality but also by environmental factors and reproduction success. The synergistic effect of odours from conspecifics and plants can enhance the attractiveness and hence the survival of the specie. Pheromone and kairomone response from two Coleopterans, tree Lepidoptera and two Homopteran insects, that are severe pest on potato, are here described as well as their behaviour towards potato crop. Knowledge about their response to semiochemicals gives indications of how to develop future crop protection management. The enhancement of sex attraction induced by host odors suggests that more effective traps can be devised for management of insect pests
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| 6. |
- Karlsson, Miriam Frida
(författare)
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Role of semiochemical in host finding, oviposition and sexual communication in Guatemalan potato moth Tecia solanivora
- 2011
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Doktorsavhandling (övrigt vetenskapligt/konstnärligt)abstract
- Semiochemicals are important cues in the interaction between plant and insects and between conspecific insects. Volatile compounds emitted by plants provide herbivorous insects with cues for host finding, selection and discrimination. In moths, female emitted sex pheromones enable conspecific males to find them for mating. This thesis investigated the role of semiochemicals in the behaviour of the Guatemalan potato moth Tecia solanivora (Lepidoptera: Gelechiidae), a pest insect of potato. Identification of odours of foliage, flowers, and tubers of potato, Solanum tuberosum, were done with coupled gas chromatography-mass spectrometry and with high-performance liquid chromatography for non-volatile compounds in tubers. Antennal activity of potato volatiles was tested with electroantennographic recordings. Attraction of T. solanivora to potato volatile compounds was investigated through olfactometer, wind tunnel and field bioassays. Male behavior towards two different synthetic pheromone blends was similarly tested, to clarify their mode of action in mating disruption management. Potato emits structure-specific volatile blends that change during the development of the plant. Tuberization stage was the preferred stage for oviposition while foliage released deterrent compounds. A three-component flower-odour mimic attracted males and females, virgin and mated, and enhanced the number of eggs laid. Female oviposition in the soil, during the tuberization stage might be guided by odours from spatially separated flowers, as an indication of suitable host vicinity. Larval survival was low in tubers with high concentrations of glycoalkaloids. This study demonstrates that odours from qualitatively different sites guide female to oviposit on tubers with high suitability for larval performance. Mating disruption was obtained with pheromone-permeated air with the two blends, but the disruption mechanism were different between them. This first study on chemical communication between T. solanivora and its host plant showed that potato volatile compounds are perceived by the moth and act as cues in host location and oviposition. It highlights the possibility of using semiochemicals to manipulate the behavior of the moths and provides a base for further investigation and development of odour-based pest management.
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