| 1. |
- Lindblad-Toh, Kerstin, et al.
(author)
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Genome sequence, comparative analysis and haplotype structure of the domestic dog.
- 2005
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In: Nature. - : Springer Science and Business Media LLC. - 1476-4687 .- 0028-0836. ; 438:7069, s. 803-19
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Journal article (peer-reviewed)abstract
- Here we report a high-quality draft genome sequence of the domestic dog (Canis familiaris), together with a dense map of single nucleotide polymorphisms (SNPs) across breeds. The dog is of particular interest because it provides important evolutionary information and because existing breeds show great phenotypic diversity for morphological, physiological and behavioural traits. We use sequence comparison with the primate and rodent lineages to shed light on the structure and evolution of genomes and genes. Notably, the majority of the most highly conserved non-coding sequences in mammalian genomes are clustered near a small subset of genes with important roles in development. Analysis of SNPs reveals long-range haplotypes across the entire dog genome, and defines the nature of genetic diversity within and across breeds. The current SNP map now makes it possible for genome-wide association studies to identify genes responsible for diseases and traits, with important consequences for human and companion animal health.
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| 2. |
- Lundström, Robert, et al.
(author)
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Fatigue modeling as related to flexible pavement design - State of the art
- 2007
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In: International Journal on Road Materials and Pavement Design. - : Informa UK Limited. - 1468-0629 .- 2164-7402. ; 8:2, s. 165-205
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Research review (peer-reviewed)abstract
- A literature study of rheological and fatigue modeling of asphalt mixtures is presented. Theoretical aspects on structural modeling, rheological behavior and the fatigue integration in design procedures are reviewed In principle, pavement design methods can be categorized in three broad groups: empirical, semi-mechanistic and fully mechanistic methods. Pavement design is generally performed using semi-mechanistic methods comprising analytical or numerical structural response models and deterioration modeling based on transfer functions and shift factors. In the case of fatigue deterioration, several approaches have been elaborated e.g. classical models, fracture mechanics and damage mechanics. The approaches differ regarding theoretical foundation and evaluation methods used. Recognizing significant limitations concerning theoretical basis as well as lack of empirical support for current design methods, a shift in paradigm from semi-empirical methods towards more advanced fully mechanistic methods have been initiated. According to this approach, improved pavements are achieved by appropriate design methods which are capable of predicting fatigue resistance in the actual pavement environment, and thus taking into account complex stress conditions, influence of temperature and material characteristics, such as aging and healing.
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| 3. |
- Lundström, Robert, et al.
(author)
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Influence of pavement materials on field performance : Evaluation of rutting on flexible semi-rigid and rigid test sections after 7 years of service
- 2009
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In: International Journal on Road Materials and Pavement Design. - : Informa UK Limited. - 1468-0629 .- 2164-7402. ; 10:4, s. 689-713
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Journal article (peer-reviewed)abstract
- This paper presents results obtained so far from a Swedish full-scale test, comprising in total 19 different rigid, semi-rigid and flexible test stretches. The purpose is to compare rutting performance of the different pavement categories and types using extensive laboratory tests and field measurements after seven years of trafficking. The rigid pavements withstand rutting better than the two other pavement categories due to comparably small abrasion and negligible permanent deformation. The semi-rigid pavements show less rutting than the flexible pavements, which primarily is the result of the negligible permanent deformation in the cement bound layers. Even though the rigid pavements show very small rutting, it is probably not the main distress type and the primary trigger for maintenance activities for this pavement type. In the case of semi-flexible and flexible pavements, both laboratory tests and field measurements indicate that significantly improved rutting performance can be obtained using appropriate asphalt mixtures manufactured using wear-resistant aggregates and stiff binder.
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| 4. |
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| 5. |
- Acciari, V. A., et al.
(author)
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Radio Imaging of the Very-High-Energy gamma-Ray Emission Region in the Central Engine of a Radio Galaxy
- 2009
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In: Science. - : American Association for the Advancement of Science (AAAS). - 0036-8075 .- 1095-9203. ; 325:5939, s. 444-448
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Journal article (peer-reviewed)abstract
- The accretion of matter onto a massive black hole is believed to feed the relativistic plasma jets found in many active galactic nuclei (AGN). Although some AGN accelerate particles to energies exceeding 10(12) electron volts and are bright sources of very-high-energy (VHE) gamma-ray emission, it is not yet known where the VHE emission originates. Here we report on radio and VHE observations of the radio galaxy Messier 87, revealing a period of extremely strong VHE gamma-ray flares accompanied by a strong increase of the radio flux from its nucleus. These results imply that charged particles are accelerated to very high energies in the immediate vicinity of the black hole.
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| 6. |
- Andersen, Felicie F., et al.
(author)
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Assembly and structural analysis of a covalently closed nano-scale DNA cage
- 2008
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In: Nucleic Acids Research. - : Oxford University Press (OUP). - 0305-1048 .- 1362-4962. ; 36:4, s. 1113-1119
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Journal article (peer-reviewed)abstract
- The inherent properties of DNA as a stable polymer with unique affinity for partner mols. detd. by the specific Watson-Crick base pairing makes it an ideal component in self-assembling structures. This has been exploited for decades in the design of a variety of artificial substrates for investigations of DNA-interacting enzymes. More recently, strategies for synthesis of more complex two-dimensional (2D) and 3D DNA structures have emerged. However, the building of such structures is still in progress and more experiences from different research groups and different fields of expertise are necessary before complex DNA structures can be routinely designed for the use in basal science and/or biotechnol. Here we present the design, construction and structural anal. of a covalently closed and stable 3D DNA structure with the connectivity of an octahedron, as defined by the double-stranded DNA helixes that assembles from eight oligonucleotides with a yield of .apprx.30%. As demonstrated by Small Angle X-ray Scattering and cryo-Transmission Electron Microscopy analyses the eight-stranded DNA structure has a central cavity larger than the apertures in the surrounding DNA lattice and can be described as a nano-scale DNA cage, Hence, in theory it could hold proteins or other bio-mols. to enable their investigation in certain harmful environments or even allow their organization into higher order structures.
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| 7. |
- Berglund, Anders, et al.
(author)
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E-readiness of University Divisions in Online Education
- 2006
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In: Netlearning 06', Ronneby.
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Conference paper (peer-reviewed)abstract
- E-readiness can be defined as the degree to which a community is prepared to participate in the networked world. In this paper the concept of e-readiness is used in terms of how internal and external factors affect the delivery of online education offered by universities. The paper applies the macro level five forces model as adopted by Chan and Welebir (2003) in the context of micro (university divisional level). Thus, the purpose is not to have generalizable findings, but rather use the delivery of online education as determinant for the level of universities' e-readiness, and explore the factors affecting e-readiness and ways of utilizing the factors as central to the study. Using a qualitative method case study interviews on divisional level were used to obtain in-depth empirical evidence. The study appears to indicate potential need to further modify the five forces model by Chan and Welebir (2003) due to the non-commercial natureof the Swedish education system. Also, co-operation in providing educational services such as the Net University in Sweden precludes market forces determination by universities internally. No specialized training program for the instructors to fit any special needs of students particular to online education was perceived.
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| 8. |
- Bylesjö, Max, et al.
(author)
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Integrated analysis of transcript, protein and metabolite data to study lignin biosynthesis in hybrid aspen
- 2009
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In: Journal of Proteome Research. - : American Chemical Society (ACS). - 1535-3893 .- 1535-3907. ; 8:1, s. 199-210
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Journal article (peer-reviewed)abstract
- Tree biotechnology will soon reach a mature state where it will influence the overall supply of fiber, energy and wood products. We are now ready to make the transition from identifying candidate genes, controlling important biological processes, to discovering the detailed molecular function of these genes on a broader, more holistic, systems biology level. In this paper, a strategy is outlined for informative data generation and integrated modeling of systematic changes in transcript, protein and metabolite profiles measured from hybrid aspen samples. The aim is to study characteristics of common changes in relation to genotype-specific perturbations affecting the lignin biosynthesis and growth. We show that a considerable part of the systematic effects in the system can be tracked across all platforms and that the approach has a high potential value in functional characterization of candidate genes.
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| 9. |
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| 10. |
- Haas, Brian J., et al.
(author)
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Genome sequence and analysis of the Irish potato famine pathogen Phytophthora infestans
- 2009
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In: Nature. - : Springer Science and Business Media LLC. - 0028-0836 .- 1476-4687. ; 461:7262, s. 393-398
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Journal article (peer-reviewed)abstract
- Phytophthora infestans is the most destructive pathogen of potato and a model organism for the oomycetes, a distinct lineage of fungus-like eukaryotes that are related to organisms such as brown algae and diatoms. As the agent of the Irish potato famine in the mid-nineteenth century, P. infestans has had a tremendous effect on human history, resulting in famine and population displacement(1). To this day, it affects world agriculture by causing the most destructive disease of potato, the fourth largest food crop and a critical alternative to the major cereal crops for feeding the world's population(1). Current annual worldwide potato crop losses due to late blight are conservatively estimated at $6.7 billion(2). Management of this devastating pathogen is challenged by its remarkable speed of adaptation to control strategies such as genetically resistant cultivars(3,4). Here we report the sequence of the P. infestans genome, which at similar to 240 megabases (Mb) is by far the largest and most complex genome sequenced so far in the chromalveolates. Its expansion results from a proliferation of repetitive DNA accounting for similar to 74% of the genome. Comparison with two other Phytophthora genomes showed rapid turnover and extensive expansion of specific families of secreted disease effector proteins, including many genes that are induced during infection or are predicted to have activities that alter host physiology. These fast-evolving effector genes are localized to highly dynamic and expanded regions of the P. infestans genome. This probably plays a crucial part in the rapid adaptability of the pathogen to host plants and underpins its evolutionary potential.
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