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- Gabrielsson, Britt, 1957, et al.
(författare)
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Molecular characterization of a local sulfonylurea system in human adipose tissue.
- 2004
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Ingår i: Molecular and cellular biochemistry. - 0300-8177 .- 1573-4919. ; 258:1-2, s. 65-71
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Tidskriftsartikel (refereegranskat)abstract
- ATP-sensitive potassium (KATP) channels are present in many cell types and link cellular metabolism to the membrane potential. These channels are heterooctamers composed of two subunits. The sulfonylurea receptor (SUR) subunits are targets for drugs that are inhibitors or openers of the KATP channels, while the inwardly rectifying K+ (Kir) subunits form the ion channel. Two different SUR genes (SUR1 and SUR2) and two different Kir6.x genes (Kir6.1 and Kir6.2) have been identified. In addition, isoforms of SUR2, SUR2A and SUR2B, have been described. We have previously performed expression profiling on pooled human adipose tissue and found high expression of SUR2. Others have reported expression of SUR1 in human adipocytes. The aim of this study was to characterize the expression of the sulfonylurea receptor complex components in human adipose tissue. RT-PCR analysis, verified by restriction enzyme digestions and DNA sequencing, showed that SUR2B, Kir6.1 and alpha-endosulfine, but not SUR1, SUR2A or Kir6.2, are expressed in human adipose tissue. Real-time RT-PCR showed that SUR2B was expressed at higher levels in subcutaneous compared with omental adipose tissue in paired biopsies obtained from seven obese men (p < 0.05). Analysis of tissue distribution showed that SUR2B expression in adipose tissue was lower than that in muscle, similar to that in heart and liver, while the expression in pancreas was lower. The effect of caloric restriction was tested in obese men (n = 10) treated with very low calorie diet for 16 weeks, followed by a gradual reintroduction of ordinary food for 2 weeks. Biopsies were taken at week 0, 8 and 18. There was no consistent effect of weight reduction on SUR2B or Kir6.1 expression. We conclude that the necessary components for a local sulfonylurea system are expressed in human adipose tissue and that the sulfonylurea receptor complex in this tissue is composed of SUR2B and Kir6.1. The expression of SUR2B was higher in subcutaneous compared with omental adipose tissue and was not affected by weight loss.
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- Grenklo, Staffan, 1959-
(författare)
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Cross-linked Profilin:actin - A tool to study actin dynamics in non-muscle cells
- 2004
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Doktorsavhandling (övrigt vetenskapligt/konstnärligt)abstract
- The microfilament system, consisting of actin and a number of auxiliary proteins, is fundamental for cell motility. Its dynamic organization depends on receptor-mediated signals, leading to rapid polymerizations and depolymerizations of actin. Profilin binds to non-filamentous actin, inhibits spontaneous filament formation, and functions as a regulator of actin polymerization. The profilin:actin complex, is thought to be the principal source of actin for filament formation although the role of profilin is not fully elucidated. In this thesis, a cross-linked profilin:actin complex (PxA), that retains the properties of ordinary profilin:actin, except for being non-dissociable, has been used to characterize the role of profilin and profilin:actin in non-muscle cells. A rapid screening method, employing PxA and based on the far western technique and mass-spectrometry, was designed to identify cellular components that specifically bind profilin:actin. Microinjection of PxA into cells infected with the bacteria Listeria monocytogenes impaired bacterial motility but a mutant PxA, unable to bind proline-rich sequences had no effect, demonstrating that profilin:actin is vital for the activity of the actin polymer-forming complex that the pathogen recruits to its surface upon infection. Fluorescence microscopy using two distinct sets of affinity-purified actin and profilin antibodies generated against PxA enabled localization of monomeric actin in cells. One of the actin and both profilin antibodies resulted in a dotted pattern of fluorescence partially aligning with microtubules whereas the other actin antibody detected filamentous actin. The result demonstrates extensive variability in epitope recognition, and indicates that unpolymerized actin, i.e. profilin:actin and maybe other complex-bound forms of actin, distributes in small packages that might be transported along microtubules. Microinjection of PxA into lamprey axons demonstrated the involvement of actin polymerization during synaptic signaling.
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- Karlsson, Staffan, 1959-, et al.
(författare)
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Elderly receiving informal and formal care or formal care only with regard to functional ability and complaints : Book of Abstracts
- 2004
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Ingår i: 12th Biennial Conference of the Workgroup of European Nurse Researchers – Nursing Research, Nursing's <contribution for Health of European Citizens. ; , s. 88-88
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Konferensbidrag (refereegranskat)abstract
- Besides the professional health and elderly care also provision of informal care is extensive to elderly. The aim was to describe and compare elderly people living at home and in special accommodation receiving informal and formal care or formal care only, with regard to functional ability and health complaints. Data was drawn from elderly people living in the southern part of Sweden (n=1963). Data covered the ”ADL-staircase” (Hulter-Asberg 1989) to measure Activity in Daily Living and cognitive functional ability (Berger 1980).Elderly living in special accommodation were older (mean age 85) than those living at home (mean age 82, p <0,001) and more often women (71 % and 66 % respectively, p <0,015). Informal care with regard to PADL and IADL were more common among those living at home than in special accommodation (PADL 24 % and 3 %, p <0,001; concerning IADL 64 % and 11 %, p <0,001). Mostly children gave informal help in IADL and the spouse in PADL, 33 % and 14 % respectively. Elderly at home with informal and formal care had significantly higher extent of walking problems, urinary incontinence and slow healing wounds than those with only formal care (p <0,043 and <0,001). Having informal care at home was associated with dependence for shopping food, transportation, and activities in social life, but also with urinary incontinence. Formal care only at home was associated with dependency in cleaning and bathing as well as faeces incontinence.
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- Karlsson, Staffan, 1959-, et al.
(författare)
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Older peoples care consumption from municipalities, County Council (CC), and informal care
- 2004
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Ingår i: Advanced health sciences of tomorrow. ; , s. 22-22
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Konferensbidrag (refereegranskat)abstract
- Objectives: The aim was to investigate care consumption in CC, municipalities and informal care for people 65+, make comparisons between age groups, and with regard to housing and informal care.Methods: The study design was cross-sectional. Data was drawn from elderly (n=1958) living in four municipalities in the southern part of Sweden and having municipal care. Data were collected regarding demography, functional abilities, and professional and informal care, and was linked to the CC administrative register including hospital care and outpatient care during year 2001.Results: 49 % got care in special accommodation (SA). The oldest (85+) had more help in PADL from home service care than the youngest elderly (65-74) but less visits/month by nurses in home nursing care compared to the youngest elderly. Primary health care visits were more frequent among the oldest, and visit to psychiatry and specialist care was more frequent among the youngest elderly. The frequency of help in IADL from spouse was lower in the oldest group. More elderly in SA had contact with primary health care by physician, compared to elderly at home. Outpatient specialist care and hospital care were more frequent among elderly living at home in contrast to SA.Conclusion: Elderly (65+) is not a homogenous group with regard to care consumption. The youngest elderly consumes more of specialised care while the oldest consumes more of primary health care. In the same way persons living at home has a higher consumption of specialised care than those living in SA. If those differences are due to marginalisation of the oldest old or due to characteristics addressed to SA remains to be explained.
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