Sökning: WFRF:(Keymeulen Bart)
> (2015) >
Plasma GAD65, a Mar...
Plasma GAD65, a Marker for Early beta-Cell Loss After Intraportal Islet Cell Transplantation in Diabetic Patients
-
Ling, Zhidong (författare)
-
De Pauw, Pieter (författare)
-
Jacobs-Tulleneers-Thevissen, Daniel (författare)
-
visa fler...
-
Mao, Rui (författare)
-
Gillard, Pieter (författare)
-
Hampe, Christiane S. (författare)
-
Martens, Geert A. (författare)
-
Veld, Peter In't (författare)
-
- Lernmark, Åke (författare)
- Lund University,Lunds universitet,Celiaki och diabetes,Forskargrupper vid Lunds universitet,Celiac Disease and Diabetes Unit,Lund University Research Groups
-
Keymeulen, Bart (författare)
-
Gorus, Frans (författare)
-
Pipeleers, Daniel (författare)
-
visa färre...
-
(creator_code:org_t)
- The Endocrine Society, 2015
- 2015
- Engelska.
-
Ingår i: Journal of Clinical Endocrinology and Metabolism. - : The Endocrine Society. - 1945-7197 .- 0021-972X. ; 100:6, s. 2314-2321
- Relaterad länk:
-
http://dx.doi.org/10...
-
visa fler...
-
https://academic.oup...
-
https://lup.lub.lu.s...
-
https://doi.org/10.1...
-
visa färre...
Abstract
Ämnesord
Stäng
- Context and Objective: Intraportal islet transplantation can restore insulin production in type 1 diabetes patients, but its effect is subject to several interfering processes. To assess the influence of beta-cell loss before and during engraftment, we searched for a real-time marker of beta-cell destruction. Previous studies showed that 65-kDa isoform of glutamate decarboxylase (GAD65) is discharged by chemically damaged rat beta-cells. We therefore examined the utility of the GAD65 assay to detect and quantify destruction of human beta-cells in vitro and in vivo. Design and Participants: A time-resolved fluorescence immunoassay was used to measure GAD65 discharge from beta-cells after administration of toxins or after intraportal transplantation. The study in patients involved type 1 diabetes recipients of 56 implants. Results: GAD65 was discharged from cultured human beta-cells between 4 and 24 hours after acute insult and proportional to the number of dying cells. It was also detected in plasma during the first 24 hours after intraportal transplantation of human islet cell grafts. Diabetic nude rat recipients without hyperglycemic correction exhibited higher plasma GAD65 levels than those with normalization. In type 1 diabetes recipients of grafts with 2-5 x 10(6) beta-cells per kilogram of body weight, five of six with plasma GAD65 greater than 1 ng/mL failed to increase plasma C-peptide by greater than 0.5 ng/mL at posttransplant month 2, whereas five of six with undetectable plasma GAD 65 and 15 of 19 with intermediate levels did result in such increase. Conclusion: Plasma GAD65 qualifies as a marker for early beta-cell loss after intraportal transplantation. Further studies are needed to extend its clinical utility.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Endokrinologi och diabetes (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Endocrinology and Diabetes (hsv//eng)
Publikations- och innehållstyp
- art (ämneskategori)
- ref (ämneskategori)
Hitta via bibliotek
Till lärosätets databas
- Av författaren/redakt...
-
Ling, Zhidong
-
De Pauw, Pieter
-
Jacobs-Tulleneer ...
-
Mao, Rui
-
Gillard, Pieter
-
Hampe, Christian ...
-
visa fler...
-
Martens, Geert A ...
-
Veld, Peter In't
-
Lernmark, Åke
-
Keymeulen, Bart
-
Gorus, Frans
-
Pipeleers, Danie ...
-
visa färre...
- Om ämnet
-
- MEDICIN OCH HÄLSOVETENSKAP
-
MEDICIN OCH HÄLS ...
-
och Klinisk medicin
-
och Endokrinologi oc ...
- Artiklar i publikationen
-
Journal of Clini ...
- Av lärosätet
-
Lunds universitet