| 1. |
|
|
| 2. |
- Ankerst, J., et al.
(författare)
-
Cytotoxicity induced by mixtures of adenovirus and specific antibodies - Induction of cytotoxic aggregates by homotypic anti-fibre sera and neutralization of the cytotoxicity by homotypic anti-hexon sera
- 1973
-
Ingår i: Archiv für die gesamte Virusforschung. - 0003-9012. ; 41:1-2, s. 99-105
-
Tidskriftsartikel (refereegranskat)abstract
- Aggregates cytotoxie for human cells in vitro were produced when adenovirus type 5 was mixed with homotypic rabbit antiserum against whole virus particles at a certain restricted virus-antibody ratio. The same degree of cytotoxicity was obtained with mixtures of the virus and an antiserum against purified fibres of adenovirus type 5 at a certain proportion. The cytotoxicity could be neutralized by homotypic antisera against purified hexons. The sequence in which the two anti-capsomer sera were added to the virus was found to be of importance: complete neutralization of the cytotoxicity appeared when virus preparations were incubated with anti-hexon sera before cytotoxic aggregates were formed by antifibre sera. In this case even a significantly lower51Cr-release appeared than that produced by corresponding amounts of virus alone. No neutralization of the cytotoxicity was obtained when anti-hexon serum was added to the mixtures after formation of cytotoxic aggregates by anti-fibre antibodies.
|
|
| 3. |
- Ankerst, J., et al.
(författare)
-
Induction of mammary fibroadenomas in rats by adenovirus type 9
- 1974
-
Ingår i: International Journal of Cancer. - : Wiley. - 0020-7136 .- 1097-0215. ; 13:3, s. 286-290
-
Tidskriftsartikel (refereegranskat)abstract
- After inoculation of adenovirus type 9 into newborn Wistar/Furth rats, seven out of seven females developed one or several mammary fibroadenomas within 14–25 weeks after virus inoculation. No tumours were observed in male rats inoculated with the same virus or in untreated controls. Neonatal inoculations of adenovirus type 5, produced on the same HeLa cells, gave negative results in both sexes. The results indicate that benign mammary tumours can be induced in rats by a virus and that mammary fibroadenomas are induced by adenovirus type 9, previously known to be capable of transforming cells in vitro but not of inducing tumours in vivo.
|
|
| 4. |
- Kjellen, L., et al.
(författare)
-
Cytotoxicity of adenovirus antibody aggregates : sensitivity to different cell strains, and inhibition by hexon antiserum and by complement
- 1973
-
Ingår i: Journal of Virology. - : American Society for Microbiology. - 0022-538X .- 1098-5514. ; 12:1, s. 25-32
-
Tidskriftsartikel (refereegranskat)abstract
- Adenovirus antibody aggregates under defined conditions are cytotoxic in vitro. All members of adenovirus groups I, II, and III caused toxicity upon aggregation. The toxicity of the clusters is exerted by the virions. Toxicity is temperature dependent and may be caused by a mechanism similar to that used in viral penetration. Cells permitting direct viral penetration were all sensitive to the toxic aggregates. The toxicity seems to be related to hexon antigens on the surface of the virions since antihexon sera neutralized the toxicity. No evidence was obtained showing that pentons are required for this kind of cytotoxicity. Adenovirus types 3,5, and 9 were used in the experiment. Cytotoxicity was estimated by the 51Cr release assay. Complement factors could be excluded as mediators of the cytolytic reactions. Instead, complement was shown to prevent the formation of toxic aggregates or to neutralize the toxicity of preformed ones.
|
|
| 5. |
- Kjellén, L., et al.
(författare)
-
Cytotoxicity of adenovirus‐antibody complexes. Specificity of antibody and virus‐antibody ratio at which cytotoxicity is induced
- 1973
-
Ingår i: European Journal of Immunology. - : Wiley. - 0014-2980 .- 1521-4141. ; 3:2, s. 78-84
-
Tidskriftsartikel (refereegranskat)abstract
- Adenoviruses aggregate in the presence of type‐specific antibodies against whole virus particles. These aggregates were found to damage human cells in vitro in far higher order of magnitude than virus alone, when virus and type‐specific antibodies were mixed at a certain virus‐antibody ratio. No cytotoxicity occurred when the virus was mixed with type‐specific antibodies in higher or lower dilutions or when the virus was incubated with antisera against another adenovirus serotype. Furthermore, it was found that the cytotoxicity could be prevented by type‐specific anti‐hexon sera and that type‐specific anti‐fiber sera produced cytotoxic aggregates out of subtoxic ones. The presence of the cytotoxic aggregates was demonstrated by electron microscopy and by 51Cr release cytotoxicity tests.
|
|
