Sökning: WFRF:(Knoepp Fenja)
> (2022) >
Essential Role of M...
Essential Role of Mitochondrial Cytochrome c Oxidase Subunit 4 Isoform 2 (Cox4i2) for Acute Pulmonary Oxygen Sensing
-
- Giordano, Luca (författare)
- Department of Internal Medicine, Universities of Giessen and Marburg Lung Center, Member of the German Center for Lung Research, Excellence Cluster Cardio-Pulmonary Institute, Justus-Liebig University, Giessen, Germany
-
- Nolte, Anika (författare)
- Department of Internal Medicine, Universities of Giessen and Marburg Lung Center, Member of the German Center for Lung Research, Excellence Cluster Cardio-Pulmonary Institute, Justus-Liebig University, Giessen, Germany
-
- Wittig, Ilka (författare)
- Faculty of Medicine Goethe-University Frankfurt, Germany
-
visa fler...
-
- Pak, Oleg (författare)
- Department of Internal Medicine, Universities of Giessen and Marburg Lung Center, Member of the German Center for Lung Research, Excellence Cluster Cardio-Pulmonary Institute, Justus-Liebig University, Giessen, Germany
-
- Knoepp, Fenja (författare)
- Department of Internal Medicine, Universities of Giessen and Marburg Lung Center, Member of the German Center for Lung Research, Excellence Cluster Cardio-Pulmonary Institute, Justus-Liebig University, Giessen, Germany
-
- Ramser, Kerstin (författare)
- Luleå tekniska universitet,Strömningslära och experimentell mekanik
-
- Wahl, Joel (författare)
- Luleå tekniska universitet,Strömningslära och experimentell mekanik
-
- Huettemann, Maik (författare)
- Center for Molecular Medicine and Genetics, Wayne State University School of Medicine, Detroit, USA
-
- Grossman, Lawrence I. (författare)
- Center for Molecular Medicine and Genetics, Wayne State University School of Medicine, Detroit, USA
-
- Pecina, Petr (författare)
- Laboratory of Bioenergetics, Institute of Physiology CAS, Prague, Czech Republic
-
- Ghofrani, Hossein A. (författare)
- Department of Internal Medicine, Universities of Giessen and Marburg Lung Center, Member of the German Center for Lung Research, Excellence Cluster Cardio-Pulmonary Institute, Justus-Liebig University, Giessen, Germany
-
- Seeger, Werner (författare)
- Department of Internal Medicine, Universities of Giessen and Marburg Lung Center, Member of the German Center for Lung Research, Excellence Cluster Cardio-Pulmonary Institute, Justus-Liebig University, Giessen, Germany; Institute for Lung Health, Justus-Liebig University, Giessen, Germany
-
- Weissmann, Norbert (författare)
- Department of Internal Medicine, Universities of Giessen and Marburg Lung Center, Member of the German Center for Lung Research, Excellence Cluster Cardio-Pulmonary Institute, Justus-Liebig University, Giessen, Germany
-
- Giehl, Klaudia (författare)
- Department of Internal Medicine, Justus-Liebig University, Giessen, Germany
-
- Sommer, Natascha (författare)
- Department of Internal Medicine, Universities of Giessen and Marburg Lung Center, Member of the German Center for Lung Research, Excellence Cluster Cardio-Pulmonary Institute, Justus-Liebig University, Giessen, Germany
-
visa färre...
-
(creator_code:org_t)
- Elsevier, 2022
- 2022
- Engelska.
-
Ingår i: Biochimica et Biophysica Acta - Bioenergetics. - : Elsevier. - 0005-2728 .- 1879-2650. ; 1863:Supplement
- Relaterad länk:
-
https://urn.kb.se/re...
-
visa fler...
-
https://doi.org/10.1...
-
visa färre...
Abstract
Ämnesord
Stäng
- Mitochondrial Cytochrome c Oxidase Subunit 4 Isoform 2 (Cox4i2) is essential for acute oxygen sensing and signaling in pulmonary arterial smooth muscle cells (PASMCs) by triggering the production of superoxide during acute hypoxia [1]. However, the molecular mechanism underlying Cox4i2-dependent oxygen sensing remains elusive. We analysed oxygen-dependent respiration by high resolution respirometry, redox changes of the electron transport chain (ETC) by RAMAN spectroscopy, and supercomplex formation by blue native gel analysis of PASMCs isolated from wild type (WT) and Cox4i2-/- mice. To understand the role of Cox4i2-specific cysteine residues we determined hypoxia-induced superoxide production and oxygen affinity in a mouse epithelial cell line (CMT167 cells) overexpressing either Cox4i1, or WT Cox4i2, or Cox4i2 mutants (C41S, C55A, C109S). Respiration and supercomplex formation were similar in WT and Cox4i2-/- PASMCs. Interestingly, hypoxia-induced reduction of ETC components (NADH, ubiquinol, and reduced cytochrome c) was prevented in Cox4i2-/- PASMCs. CMT167 cells expressing either Cox4i1, or Cox4i2 mutants lacked hypoxia-induced superoxide release, which was detected only in cells expressing WT Cox4i2. In contrast, overexpression of Cox4i1, or Cox4i2, or Cox4i2 mutants did not affect oxygen affinity. Our findings suggest that Cox4i2 does not alter superoxide production by rearrangement of supercomplexes, whereas its specific cysteines are needed for the superoxide release. In conclusion, Cox4i2 plays a major role in the hypoxia-induced reduction of ETC components, likely mediated through its redox-active cysteine residues.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinsk bioteknologi -- Medicinsk bioteknologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Medical Biotechnology -- Medical Biotechnology (hsv//eng)
Nyckelord
- Experimental Mechanics
- Experimentell mekanik
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
Hitta via bibliotek
Till lärosätets databas
- Av författaren/redakt...
-
Giordano, Luca
-
Nolte, Anika
-
Wittig, Ilka
-
Pak, Oleg
-
Knoepp, Fenja
-
Ramser, Kerstin
-
visa fler...
-
Wahl, Joel
-
Huettemann, Maik
-
Grossman, Lawren ...
-
Pecina, Petr
-
Ghofrani, Hossei ...
-
Seeger, Werner
-
Weissmann, Norbe ...
-
Giehl, Klaudia
-
Sommer, Natascha
-
visa färre...
- Om ämnet
-
- MEDICIN OCH HÄLSOVETENSKAP
-
MEDICIN OCH HÄLS ...
-
och Medicinsk biotek ...
-
och Medicinsk biotek ...
- Artiklar i publikationen
-
Biochimica et Bi ...
- Av lärosätet
-
Luleå tekniska universitet