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Multi-HLA class II ...
Multi-HLA class II tetramer analyses of citrulline-reactive T cells and early treatment response in rheumatoid arthritis
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- Gerstner, Christina (författare)
- Karolinska Univ Hosp, Ctr Mol Med, Dept Med Solna, Div Rheumatol, Stockholm, Sweden.;Karolinska Inst, Stockholm, Sweden.
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- Turcinov, Sara (författare)
- Karolinska Institutet
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- Hensvold, Aase H. (författare)
- Karolinska Institutet
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- Chemin, Karine (författare)
- Karolinska Institutet
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- Uchtenhagen, Hannes (författare)
- Karolinska Univ Hosp, Ctr Mol Med, Dept Med Solna, Div Rheumatol, Stockholm, Sweden.;Karolinska Inst, Stockholm, Sweden.;BRI Virginia Mason, Translat Res Program, Seattle, WA USA.
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- Ramwadhdoebe, Tamara H. (författare)
- Univ Amsterdam, Amsterdam UMC, Dept Clin Immunol & Rheumatol, Amsterdam Infect & Immun Inst, Amsterdam, Netherlands.;Univ Amsterdam, Amsterdam UMC, Dept Expt Immunol, Amsterdam Infect & Immun Inst, Amsterdam, Netherlands.;Acad Med Ctr, Amsterdam Rheumatol & Immunol Ctr ARC, Amsterdam, Netherlands.
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- Dubnovitsky, Anatoly (författare)
- Karolinska Institutet
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- Kozhukh, Genadiy (författare)
- Karolinska Univ Hosp, Ctr Mol Med, Dept Med Solna, Div Rheumatol, Stockholm, Sweden.;Karolinska Inst, Stockholm, Sweden.
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- Rönnblom, Lars (författare)
- Uppsala universitet,Reumatologi,Science for Life Laboratory, SciLifeLab
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- Kwok, William W. (författare)
- BRI Virginia Mason, Translat Res Program, Seattle, WA USA.
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- Achour, Adnane (författare)
- Karolinska Institutet
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- Catrina, Anca (författare)
- Karolinska Univ Hosp, Ctr Mol Med, Dept Med Solna, Div Rheumatol, Stockholm, Sweden.;Karolinska Inst, Stockholm, Sweden.
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- van Baarsen, Lisa G. M. (författare)
- Karolinska Institutet
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- Malmstrom, Vivianne (författare)
- Karolinska Univ Hosp, Ctr Mol Med, Dept Med Solna, Div Rheumatol, Stockholm, Sweden.;Karolinska Inst, Stockholm, Sweden.
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Karolinska Institutet Karolinska Univ Hosp, Ctr Mol Med, Dept Med Solna, Div Rheumatol, Stockholm, Sweden;Karolinska Inst, Stockholm, Sweden. (creator_code:org_t)
- 2020-05-18
- 2020
- Engelska.
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Ingår i: BMC Immunology. - : Springer Science and Business Media LLC. - 1471-2172. ; 21:1
- Relaterad länk:
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https://doi.org/10.1...
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https://uu.diva-port... (primary) (Raw object)
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https://bmcimmunol.b...
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https://urn.kb.se/re...
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https://doi.org/10.1...
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http://kipublication...
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Abstract
Ämnesord
Stäng
- Background HLA class II tetramers can be used for ex vivo enumeration and phenotypic characterisation of antigen-specific CD4+ T cells. They are increasingly applied in settings like allergy, vaccination and autoimmune diseases. Rheumatoid arthritis (RA) is a chronic autoimmune disorder for which many autoantigens have been described. Results Using multi-parameter flow cytometry, we developed a multi-HLA class II tetramer approach to simultaneously study several antigen specificities in RA patient samples. We focused on previously described citrullinated HLA-DRB1*04:01-restricted T cell epitopes from alpha-enolase, fibrinogen-beta, vimentin as well as cartilage intermediate layer protein (CILP). First, we examined inter-assay variability and the sensitivity of the assay in peripheral blood from healthy donors (n = 7). Next, we confirmed the robustness and sensitivity in a cohort of RA patients with repeat blood draws (n = 14). We then applied our method in two different settings. We assessed lymphoid tissue from seropositive arthralgia (n = 5) and early RA patients (n = 5) and could demonstrate autoreactive T cells in individuals at risk of developing RA. Lastly, we studied peripheral blood from early RA patients (n = 10) and found that the group of patients achieving minimum disease activity (DAS28 < 2.6) at 6 months follow-up displayed a decrease in the frequency of citrulline-specific T cells. Conclusions Our study demonstrates the development of a sensitive tetramer panel allowing simultaneous characterisation of antigen-specific T cells in ex vivo patient samples including RA 'at risk' subjects. This multi-tetramer approach can be useful for longitudinal immune-monitoring in any disease with known HLA-restriction element and several candidate antigens.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Immunologi inom det medicinska området (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Immunology in the medical area (hsv//eng)
Nyckelord
- Autoimmune disease
- Autoreactive CD4+T lymphocytes
- Multi-MHC class II tetramer assay
- Citrullination
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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Till lärosätets databas
- Av författaren/redakt...
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Gerstner, Christ ...
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Turcinov, Sara
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Hensvold, Aase H ...
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Chemin, Karine
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Uchtenhagen, Han ...
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Ramwadhdoebe, Ta ...
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visa fler...
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Dubnovitsky, Ana ...
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Kozhukh, Genadiy
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Rönnblom, Lars
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Kwok, William W.
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Achour, Adnane
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Catrina, Anca
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van Baarsen, Lis ...
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Malmstrom, Vivia ...
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BMC Immunology
- Av lärosätet
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Uppsala universitet
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Karolinska Institutet