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A regular pattern o...
A regular pattern of Ig super-motifs defines segmental flexibility as the elastic mechanism of the titin chain
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- von Castelmur, Eleonore (författare)
- Division of Structural Biology, Biozentrum, University of Basel, Basel, Switzerland
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- Marino, Marco (författare)
- Division of Structural Biology, Biozentrum, University of Basel, Basel, Switzerland
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- Svergun, Dmitri I (författare)
- European Molecular Biology Laboratory, Hamburg Outstation, c/o Deutsches Elektronen Synchrotron (DESY), Hamburg, Germany; Institute of Crystallography, Russian Academy of Sciences, Moscow, Russia
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- Kreplak, Laurent (författare)
- M. E. Müller Institute for Structural Biology, Biozentrum, University of Basel, Basel, Switzerland
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- Ucurum-Fotiadis, Zöhre (författare)
- Division of Structural Biology, Biozentrum, University of Basel, Basel, Switzerland
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- Konarev, Petr V (författare)
- European Molecular Biology Laboratory, Hamburg Outstation, c/o Deutsches Elektronen Synchrotron (DESY), Hamburg, Germany; Institute of Crystallography, Russian Academy of Sciences, Moscow, Russia
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- Urzhumtsev, Alexandre (författare)
- University-Nancy, Vandoeuvre-les-Nancy, France; Institut de Génétique et de Biologie Moléculaire et Cellulaire, Centre National de la Recherche Scientifique-Institut National de la Santé et de la Recherche Médicale-Université Louis Pasteur, Illkirch, France
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- Labeit, Dietmar (författare)
- Institut für Anästhesiologie und Operative Intensivmedizin, Universitätsklinikum Mannheim, Mannheim, Germany
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- Labeit, Siegfried (författare)
- Institut für Anästhesiologie und Operative Intensivmedizin, Universitätsklinikum Mannheim, Mannheim, Germany
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- Mayans, Olga (författare)
- Division of Structural Biology, Biozentrum, University of Basel, Basel, Switzerland; Institut für Anästhesiologie und Operative Intensivmedizin, Universitätsklinikum Mannheim, Mannheim, Germany
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(creator_code:org_t)
- 2008-01-29
- 2008
- Engelska.
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Ingår i: Proceedings of the National Academy of Sciences of the United States of America. - Washington, DC, United States : National Academy of Sciences. - 0027-8424 .- 1091-6490. ; 105:4, s. 1186-1191
- Relaterad länk:
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http://www.pnas.org/...
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- Myofibril elasticity, critical to muscle function, is dictated by the intrasarcomeric filament titin, which acts as a molecular spring. To date, the molecular events underlying the mechanics of the folded titin chain remain largely unknown. We have elucidated the crystal structure of the 6-Ig fragment I65-I70 from the elastic I-band fraction of titin and validated its conformation in solution using small angle x-ray scattering. The long-range properties of the chain have been visualized by electron microscopy on a 19-Ig fragment and modeled for the full skeletal tandem. Results show that conserved Ig-Ig transition motifs generate high-order in the structure of the filament, where conformationally stiff segments interspersed with pliant hinges form a regular pattern of dynamic super-motifs leading to segmental flexibility in the chain. Pliant hinges support molecular shape rearrangements that dominate chain behavior at moderate stretch, whereas stiffer segments predictably oppose high stretch forces upon full chain extension. There, librational entropy can be expected to act as an energy barrier to prevent Ig unfolding while, instead, triggering the unraveling of flanking springs formed by proline, glutamate, valine, and lysine (PEVK) sequences. We propose a mechanistic model based on freely jointed rigid segments that rationalizes the response to stretch of titin Ig-tandems according to molecular features.
Ämnesord
- NATURVETENSKAP -- Kemi (hsv//swe)
- NATURAL SCIENCES -- Chemical Sciences (hsv//eng)
Nyckelord
- electron microscopy
- poly-Ig tandem structure
- small angle x-ray scattering
- titin elasticity
- x-ray crystallography
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von Castelmur, E ...
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Marino, Marco
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Svergun, Dmitri ...
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Kreplak, Laurent
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Ucurum-Fotiadis, ...
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Konarev, Petr V
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Urzhumtsev, Alex ...
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Labeit, Dietmar
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Labeit, Siegfrie ...
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Mayans, Olga
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