| 1. |
- Benstead, Lindsay, et al.
(författare)
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Using Tablet Computers to Implement Surveys in Challenging Environments
- 2017
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Ingår i: Survey Practice. - 2168-0094. ; 10:2
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Tidskriftsartikel (refereegranskat)abstract
- Computer-assisted personal interviewing (CAPI) has increasingly been used in developing countries, but literature and training on best practices have not kept pace. Drawing on our experiences using CAPI to implement the Local Governance Performance Index (LGPI) in Tunisia and Malawi and an election study in Jordan, this paper makes practical recommendations for mitigating challenges and leveraging CAPI’s benefits to obtain high quality data. CAPI offers several advantages. Tablets facilitate complex skip patterns and randomization of long question batteries and survey experiments, which helps to reduce measurement error. Tablets’ global positioning system (GPS) technology reduces sampling error by locating sampling units and facilitating analysis of neighborhood effects. Immediate data uploading, time-stamps for individual questions, and interview duration capture allowed real time data quality checks and interviewer monitoring. Yet, CAPI entails challenges, including costs of learning new software; questionnaire programming; and piloting to resolve coding bugs; and ethical and logistical considerations, such as electricity and Internet connectivity.
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| 2. |
- Benstein, Ruben M., et al.
(författare)
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Isolation of nuclei tagged in specific cell types (INTACT) in Arabidopsis
- 2023. - 2
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Ingår i: Flower development. - New york : Humana Press. - 9781071632987 - 9781071632994 ; , s. 313-328
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Bokkapitel (refereegranskat)abstract
- Many functionally distinct plant tissues have relatively low numbers of cells that are embedded within complex tissues. For example, the shoot apical meristem (SAM) consists of a small population of pluripotent stem cells surrounded by developing leaves and/or flowers at the growing tip of the plant. It is technically challenging to collect enough high-quality SAM samples for molecular analyses. Isolation of Nuclei Tagged in specific Cell Types (INTACT) is an easily reproducible method that allows the enrichment of biotin-tagged cell-type-specific nuclei from the total nuclei pool using biotin-streptavidin affinity purification. Here, we provide a detailed INTACT protocol for isolating nuclei from the Arabidopsis SAM. One can also adapt this protocol to isolate nuclei from other tissues and cell types for investigating tissue/cell-type-specific transcriptome and epigenome and their changes during developmental programs at a high spatiotemporal resolution. Furthermore, due to its low cost and simple procedures, INTACT can be conducted in any standard molecular laboratory.
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| 3. |
- di Bari, R., et al.
(författare)
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Buildings LCA and digitalization: Designers' toolbox based on a survey
- 2022
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Ingår i: IOP Conference Series: Earth and Environmental Science. - : IOP Publishing. - 1755-1307 .- 1755-1315. ; 1078:1
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Konferensbidrag (refereegranskat)abstract
- In a context of digitalization and increasing quality requirements, the building sector is facing an increasing level of complexity regarding its design process. This results in a growing number of involved actors from different domains, a multitude of tasks to be completed and a higher degree of needed expertise. New buildings are also required to reach higher performances in terms of environmental quality. To that regard, the exploitation of the full potential of digital tools can facilitate the integration of environmental aspects in the planning process, limit productivity shortcomings and reduce environmental impacts, which can result from an unaware decision making. Building environmental assessment can be performed through several Life Cycle Assessment (LCA)-based tools. “Pure calculation” tools quantify final buildings' environmental potential, while “complex tools” additionally support decision making during the planning process. It is often difficult to choose the best suitable tool, which strongly depends on the user's needs. Within the IEA EBC Annex 72, a survey was realized with the main objective of creating a comprehensive overview of the existing tools dedicated to buildings LCA. The questionnaire included the usability, functionality, compliance, data reliability and interoperability of the analysed tools. Lastly, based on the survey outcomes and their critical assessment, a procedure for the identification and selection of a tool has been proposed based on user's needs. As a result, this work outlines main features of currently available building LCA tools, for which there is a harmonized status in terms of usability and overall applied LCA methodology. Despite the need for more automatized workflows, tools' embedding is mostly not yet applicable in system chains or limited to a restricted number of tools.
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| 4. |
- Zhivotovsky, B, et al.
(författare)
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Current concepts in cell death
- 2001
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Ingår i: Current protocols in cell biology. - : Wiley. - 1934-2616 .- 1934-2500. ; Chapter 18, s. 18.1-
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Tidskriftsartikel (refereegranskat)
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