Ribosomal protein neighborhoods : I. 818 and S21 as well as S5 and S8 are Neighbors

• The principal objective of the present experiments is to demonstrate the use of antibodies, raised against individual ribosomal proteins, to identify the member proteins of complexes created by treating ribosomes with bifunctional reagents. First, we verified our previous report that S18 and S21 can be crosslinked by treating 30S subunits with phenylenedimaleimide. Then, we used antibodies to identify the members of a complex obtained by treating 30S subunits with dimethyladipimidate; these are S5 and S8. We tentatively conclude that S18 and S21 as well as S5 and S8 are near neighbors in the 30S ribosomal subunit.

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NATURVETENSKAP  -- Biologi (hsv//swe)

NATURAL SCIENCES  -- Biological Sciences (hsv//eng)

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